[Histonet] RE: difference between immunocytochemistry and immunohistochemistry-Fr

2009-11-30 Thread Dong Yujuan
Dear Chris Richard and Adam,   Thank you so much for your advice. I decide try more, e.g. change other fixing methods. The protein is LRRC32 and only 2 companies provide Ab. Neither of them is for IHC. If it doesn't work, I think maybe I will generate the polyclonal Ab by myself.   C

[Histonet] Re: a basic question about immunohistochemistry (IHC ON DEAD RAT)

2009-11-30 Thread Jamie E Erickson
Dear Salim, I have done lots of IHC on mouse and rat tissues ie brain and trying to make correlations on viable tissue is hard enough. If you do preform the IHC it may confound your data one way or the other and leave you with more questions then answers. My recommendation would be to leave

Re: [Histonet] (no subject)

2009-11-30 Thread Peter Carroll
Unsuscribe, please. In the ever prescient words of Roger Moretz... First of all, that's not even a word. Secondly, that's not the way it's done. I'm sure others will point you in the right direction... ;) ___ Histonet mailing list

[Histonet] FTE Workload

2009-11-30 Thread Jeanne Clark
Can you please send me information on justifying FTE per workload.  Our facility has not increased in case number but the complexity and block# per case has increased.  It is hard to make a non-histology administrative person understand how you cannot compare workload in Pathology against

Re: [Histonet] FTE Workload

2009-11-30 Thread Rene J Buesa
Under separate cover I am sending you an article about staffing in histology. René J. --- On Mon, 11/30/09, Jeanne Clark paintedsplas...@yahoo.com wrote: From: Jeanne Clark paintedsplas...@yahoo.com Subject: [Histonet] FTE Workload To: histonet@lists.utsouthwestern.edu Date: Monday, November

[histonet] Cleaning and re-using slides

2009-11-30 Thread Nicholas Evans
Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are

Re: [histonet] Cleaning and re-using slides

2009-11-30 Thread Peter Carroll
sorry... your boss is crazy! ;) Nicholas Evans wrote: Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and

[Histonet] Cap for Centra GP8R swinging bucket

2009-11-30 Thread Rathborne, Toni
Hi everyone, I need your help. We have a Thermo IEC centrifuge which I need two bucket lids for. Catalog # 6377, but I have been told by the manufacturer that the item has been discontinued, and their stock supply of that part has been depleted. If there is anyone who may have this item

RE: [histonet] Cleaning and re-using slides

2009-11-30 Thread Smith, Allen
Cleaning slides strikes me as a very poor use of student time. If they are paid even 3/4 minimum wage, it is uneconomical. If they are unpaid, it is unfair to set them a task they can learn nothing from. -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric

[Histonet] Anything New for Finding Lymph Nodes in Colon Fat?

2009-11-30 Thread Smith Wanda
Has anyone discovered anything new since the last discussion in the archives for finding lymph nodes in colonic fat??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email

[Histonet] Morgue topic: Chain of Custody for personal effects

2009-11-30 Thread Greg Dobbin
Hi Folks, Can any of you share with me your procedure for ensuring chain of custody for personal effects of decedents. Along with documenting a clear procedure for all stakeholders to abide by, I also need to solve some the practical aspects like what to use for a tamper-resistant bag for the

RE: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat?

2009-11-30 Thread Truscott, Tom
Hi Wanda, Soaking tissues in white vinegar tends to clear the fat and make the lymph nodes more visible, but it might affect some tests. Tom Truscott -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

Re: [histonet] Cleaning and re-using slides

2009-11-30 Thread Rene J Buesa
Place the slides in a hot (90ºC) of a 2%dishwasherer soap solution. Repeat 3 times and the slides will be cleaned. The only problem that you will have is that the slides can stick to one another. You may want to place the used slides in racks (the same used to stain), so they will be separated

Re: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat?

2009-11-30 Thread Cliff Berger
Dissect Aid. Just Google it. -- Best regards! Cliff Berger Decal Chemical Corp 1-800-428-5856 On 11/30/09 2:58 PM, Smith Wanda wanda.sm...@hcahealthcare.com wrote: Has anyone discovered anything new since the last discussion in the archives for finding lymph nodes in colonic fat???

Re: [histonet] Cleaning and re-using slides

2009-11-30 Thread Nicholas Evans
Thanks very much everyone for the responses!!! Pretty much everyone thinks this is a ridiculous idea (to put it politely). Plus I found out that we may be overpaying for our slides - we buy Superfrost from Fisher at $350/10 gross, whereas Gorilla Scientific are selling the same type of slide for