[Histonet] Directly conjugated secondaries versus biotinylated secondaries

Greetings, Hopefully all of you are enjoying a great time with your friends and family this week rather than working like me. Here is my problem. I have an antibody (anti-panendothelial antigen, i.e. MECA32 from BD http://www.bdbiosciences.com/ptProduct.jsp?prodId=19506) and I'm trying to get it

[Histonet] Trichrome/PMMA ground sections

Randy, Our researchers like the modified Goldner's Trichrome. I used a paraffin method and modified it to work for plastic. A Toluidine Blue works well on stents, plus the other stains discussed earlier. Vicki ___ Histonet mailing list Histonet@lists

[Histonet] Happy Holidays

To all my friends and colleagues on Histonet I wish you a very Happy Holiday season filled with family and friends Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.u

[Histonet] Re: Trichrome stain for MMA and Sandersons Rapid Bone Stain chemical makeup (long reply with protocol)

Jack Ratiff wrote: You can do H&E and VVG quite nicely on this type of MMA embedded tissue. Maybe you could also try a Sanderson's (methylene blue) with the Van Gieson (acid fuchsin w/ picric acid) counterstain??? On Dec 21, 2009, at 11:51 PM, Randall Carpenter http://lists.utsouthwestern.

[Histonet] BK Virus Antibody

We used to purchase our BK virus antibody from a company called Chemicon. Apparently they were bought out by Millipore and the antibody is not available from them. I need to get the antibody from a new source. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical

[Histonet] Antigen retrieval and myosin heavy chain question

Dear Histonetters, I was wondering if anyone out there has used antigen retrieval methods to facilitate immuno-staining of formalin-fixed skeletal muscle fibers? I am using ALD-58 + ABC Elite Mouse kit to stain slow-tonic fibers in fresh amphibian muscles, but need to take a look at the fiber dis

RE: [Histonet] IHC QC slide management

We have a column on the quality control sheet we give the pathologist with the slides. The pathologist marks the column for + patient slide. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From: histonet-boun..

RE: [Histonet] sharpen permanent knife blades

I agree with most of what you have stated and espcially with sticking with one company once you get things moving in the right direction. However, if one truly knows how to properly set and/or adjust cutting angles at the microtome, I would definely keep cost at a high consideration if quality

Re: [Histonet] sharpen permanent knife blades

Different companies have varied angles on how a knife is sharpened for their equipment.  A knife sharpened by one company will generally be somewhat different in the angle of the edge than another when you begin cutting.  Some may have a flatter edge or a fatter edge at the top cutting portion

Re: [Histonet] Trichrome stain on MMA sections

You can do H&E and VVG quite nicely on this type of MMA embedded tissue. Maybe you could also try a Sanderson's (methylene blue) with the Van Gieson (acid fuchsin w/ picric acid) counterstain??? On Dec 21, 2009, at 11:51 PM, Randall Carpenter wrote: Dear Histonet, I was wondering wh

Re: [Histonet] sharpen permanent knife blades

I will also add that DDK and Dorn and Hart Microedge also sharpen knives. One should also check the pricing as they all vary. Jack Sent from my iPhone On Dec 22, 2009, at 8:07 AM, "Smith, Allen" wrote: C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens permanent microtom

RE: [Histonet] sharpen permanent knife blades

C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens permanent microtome knives. They also have two grades of disposable blades. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthw

[Histonet] sharpen permanent knife blades

Please help:-). I am looking for information on anyone who sharpens permanent knife blades. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They ar

Re: [Histonet] Re: slide printers

We are currently demoing the Fisher Slidemate slide printer and so far we love it. Works well at individual stations but probably not for large batch printing. Mark A Burton HTL ASCP Mass. General Hospital Boston, MA Quoting Lizbeth Kelly : Hi, Can someone in histoland share names of slide