How about fluorescent nissl?
2010-01-10
TF
发件人： Nicholas David Evans
发送时间： 2010-01-10 02:16:57
收件人： Adam .
抄送： histonet
主题： Re: [Histonet] Counterstain for fluorescent tissue
Dear Adam,
Thanks very much for the very helpful advice. I guess my real question, which
you're answered
Dear Histonet; my name is Steve Richardson and I am looking for your help. I
have read much in the archives regarding issues with block and slide
storage. I am looking to asses the viability of a small business dedicated
to histology block and slide storage. I would like to gather some more data
Rae Ann Staskiewicz (whrere?) asks:
>>We had a case the other day which showed poor nuclear detail. This was fatty
>>breast which had been fixed overnight before processing. Tissues were
>>reprocessed in hopes of improving, but failed to do so. Does anyone have any
>>ideas what may have caused
Dear Adam,
Thanks very much for the very helpful advice. I guess my real question, which
you're answered for me, was - is there a histological stain v similar to H&E
that isn't fluorescent itself, and which you can use without screwing up the
intrinsic fluorescence of your tissue? I'll just sti
Hi,
The most common counterstains for fluorescent work is the nuclear
counterstain DAPI, which fluoresces in the UV spectrum. If your
microparticles fluoresce in that wavelength, then you obviously can't use
that but there are a whole host of other nuclear counterstains that
fluoresce in pretty mu
One cause for poor nuclear detail is drying out of tissue. We saw this, when
we let the tissue cassettes to long without formalin before VIP-processing.
Gudrun Lang
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