Hi,
I have just encountered a problem I have never seen before and would
welcome opinions as to the cause. I work in pharma RD and had a request
to perform MSBs on some liver slides. The results varied within the run,
with a distinct difference in the overall colour of the slides; some
Hi: We are looking to start N-ras and B-raf for IHC, is anyone running
these antibodies on paraffin? If so, what clone and vendor are you using?
Thanks in advance!
--
Marian L. Powers, HT(ASCP)
Manager, Technical Operations
Doctors Pathology Services
1253 College Park Drive
Dover, DE 19904
Hi all,We also have observed this phenomenon many times. But sorry Colleen and
Rene, I don't believe that an fixation issue is the explanation why the edges
are sometimes stronger than the rest. To my opinion this is a bit too easy. One
of my explanations is that the immuno reagents tend to
Actually, when I read about the problem, my thought was that if you don't circle
your tissue with an immunopen or wax pencil, then the reagents (esp. primary
antibody)might not cover the tissue evenly as you say. So I tend to agree with
you.
Peggy
-Original Message-
From:
Again, provided that you are doing IHC manually.
René J.
--- On Tue, 1/12/10, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF
To: C.M. van der Loos c.m.vanderl...@amc.uva.nl,
Hi Dr. van der Loos:
I had also experienced that artifact caused by less than necessary reagents,
but that happens mostly when IHC is done manually, or when the autostainer
delivers less amount than required or programmed.
IF we assume that the colleague with the question did the IHC manually,
I think we'll agree that there are different scenarios so that the solution is
not a one size fits all. For example, darkened staining around the periphery of
needle core biopsies is not uncommon with even tinctorial stains, often thought
to be the result of drying of the tissue during the
I do my immuno staining manually using ProbeOn Plus slides which employ the
capillary action principle. I always monitor the taking up/whicking off of my
solutions for each pair of slides throughout the entire staining process and
know that my sections never dry out (I incubate using moist
---
Hello everyone,
Our system's hazardous chemical waste consultants changed our method of
disposal- we had been solidifying the paraffin in a hood and then red bagging
it, ie treating it as regulated medical (biohazard) waste.
But, due to it's contamination with xylene, the
