RE: [Histonet] A blast from the past....

How true! Can you believe the things we use to put down the drain or in the trash? Out of sight, out of mind. Today a high chemistry lab would be shut down and the teacher sent to jail for some of the things they taught us back then. Mike -Original Message- From:

[Histonet] Re : KP Markers

KP Marker pens are sold by Mercedes Medical. I use them and I really like them. The box I ordered is still on back order so you want to order as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] b-5

Someone remind me. (senior moment). Years ago we stopped using B-5 fixative in our lab. If I remember right we were given a date by CAP to either stop using a mercury product or present a plan on when we would stop using it. Am I correct and if not are some people still using B-5?

[Histonet] Friday Hour of Fun

I have a Hypothetical Situation for which I would like your input. If you - as a histologist - were to be involved in the interview of a potential staff pathologist, what questions would you ask this candidate? Think of the practical and logical issues, but also the unique and pertinent

RE: [Histonet] b-5

Sh, if I understand correctly, they are not legal. It was several years ago that we were mandated to stop. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday,

[Histonet] job opening

We are looking for a fulltime histologist for a new lab in Olympia, WA. Planned opening in March with great new equipment and lab space. The ideal candidate will have 3 to 5 years experience and must be able to work alone. Flexible work schedule. . Contact Konni Black kbl...@digestivehlth.com

[Histonet] Decontamination of a Cryostat

This issue seems to be rearing its ugly head in our lab once again. What is the correct procedure for decontaminating a cryostat after let's say a specimen from an HIV patient for a frozen was cut on it? We have a couple of different cryostats, one of them defrosts quickly and the other one ices

Re: [Histonet] Decontamination of a Cryostat

Hi Sharon, I copied and pasted a chemical disinfection procedure for cryostats that I created for Leica's sales and service employees and customers. It references Leica cryostats but it can be used for any cryostat--the basic principles for chemical disinfection are the same. Since we can't

[Histonet] RE: Friday Hour of Fun

I'd ask how he sees his relationship with the histotechnologists, histo supervisor, etc. I'd ask him what his expectation is regarding TAT of slides/special stains/IHC/rectus. If there is more than one Pathologist, I'd ask how they would collaborate to decide on standard work. For example, will

[Histonet] Kaposi's sarcoma block

Can anyone spare a block of Kaposi's sarcoma? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899

[Histonet] RE: Decontamination of a Cryostat

Cryostat decontamination references: Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J Histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective

[Histonet] Pathology Laboratory Manager Opening in Houston, Texas

SightLine is expanding their service lines and is seeking a pathology laboratory manager who will be responsible for the pathology laboratory services from the design and construction of the new facility, equipment purchasing, hiring staff, policies and procedures, certification and licensure.

[Histonet] RE: Decontamination of a Cryostat

What about instruments that have a built-in defrost and disinfect process with formalin? Is that sufficient to meet CAP's requirements? We do it weekly at our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

[Histonet] TUNEL

Hi all, I am trying to do an apoptosis assay on mouse lung tissue (FFPE), using the Trevigen (formerly Roche) TACs TdT system (fluorescent). I am generating a positive control using nuclease provided by the kit - and get a beautiful, clean, strong signal. Problem is, when I do the same

[Histonet] looking for Marvin Hanna

Does anyone have contact information for him? Thanks, Kaye Ryan North Florida Dermatology Associates Histology Lab Supervisor (904) 398-0547 Ext. 2004 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] TUNEL

Hi Melissa, I use the Trevigen system with great success on mouse tissue using biotinylated dNTPs, Streptavidin HRP and NovaREd. It should work just as well with fluorescence. Mouse thymus is a great positive control. My protocol is as follows: -Xylenes, ethanols, water. -Proteinase K 30

[Histonet] Processing brain biopsies on Leica Peloris

I'm looking for feedback on processing and processing programs for brain biopsy cases using the Peloris. My main focus is pediatric brain biopsy cases. We are in the stages of justifying a new processor and I need feedback regarding brain biopsy turn around time on brain biopsies. Any

[Histonet] Hypothetical Pathologist

Thank you to all who have responded to my Hypothetical Pathologist Interview scenario! I have accumulated a nice bunch of Hypothetical Questions and I have until Tuesday, Feb. 2 at 3:00 pm NM time to finish my project - I would like very much to hear from you until then. After I work over the

Re: [Histonet] Friday Hour of Fun

Sara: Even when you suggested to answer you directly I always prefer to answer to the list so anybody can agree/disagree/expand.   I don't see the point in asking a pathologist specific things histology because they usually do not train on this matters, but I would like to know:   1- what s/he

[Histonet] Klotz fixative

Do you know of anyone that sells Klotz fixative? We use it in a pre vet class but would like to eliminate the choloralhydrate since it is such a hassel due to it's being a controlled substance. Is there a substitute fixative that would retain the properties of Klotz?

[Histonet] Histology Jobs!!!!

Hi Histoneters, Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely

Re: [Histonet] Friday Hour of Fun. Answer to ,,,

YES cubed and in spades! The whole idea of Histonet is that everyone can read the questions, replies, opinions and discussion. Well said, Rene Buesa! John Kiernan Anatomy, UWO London, Canada = = = Rene J Buesa rjbu...@yahoo.com wrote: Even when you suggested to answer you directly I always