[Histonet] FITC for IgG 1,2,3,4

I have a request from a pathologist for these antibodies. I have been unable to locate a vendor in my usual places. Is anyone doing these antibodies, and if so, could you let me know a vendor (s) to check out? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager,

[Histonet] IF staining on peritoneal macrophages

Thank you Adam and Jay for your replies. Cytoslides are the pre-marked slides for use with the Cytospin - http://www.thermo.com/com/cda/product/detail/0,1055,21035,00.html Adam, do you have a recommended concentration of BSA for this? Also, do you air dry the slides prior to fixation? Do you air

Re: [Histonet] IF staining on peritoneal macrophages

When I did it, I think I used our standard sort buffer, which is 0.2% BSA in PBS pH 7.4. I've also read that you can use anything with serum, typically 5-10%. The key seems to be prewetting the membranes with something with protein. I usually cytospin them and briefly (30 seconds) air dry them

[Histonet] IF staining on peritoneal macrophages

You may want to try using the NuView Cyto prep technique from QC sciences it is a good alternative to a Cytospin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EDTA

I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank

[Histonet] Re: DRGs

DRG's for mice? Do mice have Medicare now? I hope the pending health care reform legislation addresses this issue - it's no wonder costs are spiraling out of sight! I have the feeling that a mouse DRG might be something other than Diagnosis Related Group, the coding scheme that has determined

Re: [Histonet] EDTA

EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies. The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid). By itself will produce an extremely gentle

[Histonet] Tissue repository

Dear histonet, Does anyone know where I can find a tissue repository to exchange tissue blocks? For example, I have some extra melanoma FFPE tissue blocks but would like some normal FFPE tonsil tissue blocks. I do not wish to buy blocks, but rather trade. Please let me know if there is such a

Re: [Histonet] Tissue repository

Contact the NSH that I think offers such a service. René J. --- On Fri, 2/19/10, Joao Pessoa jphistol...@gmail.com wrote: From: Joao Pessoa jphistol...@gmail.com Subject: [Histonet] Tissue repository To: histonet@lists.utsouthwestern.edu Date: Friday, February 19, 2010, 4:21 PM Dear histonet,

[Histonet] seeking histology position

Fellow techs, I am currently seeking a F/T position as a bench tech. I am ASCP certified and Florida licensed w/ a BS degree. Relocation and temp. positions are a possibility. Please feel free to pass this e mail along if you know of anyone looking for a tech. Thanks in advance Ron

[Histonet] Re: EDTA

You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine

RE: [Histonet] Re: EDTA

I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be

RE: [Histonet] Re: EDTA

://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version

Re: [Histonet] Distance learning?

You should try Harford Community College. I know several histo techs who have completed histo tech training through the Harford on-line program, and the practical or technical portions in their own lab (employer must approve). http://www.harford.edu/cet/histotech/default.asp Thanks, Joao Histo

Re: [Histonet] Re: DRGs

Good on yer, Bob! There are indeed far too many abbreviations. Anything not in the Abbreviations appendix of an ordinary dictionary (volume = one litre or less, as on the average family's shelf) should be explained. In this case my interpretation was DRG = dorsal root ganglia. Dissecting