[Histonet] FW: mouse prions

2010-02-23 Thread Edwards, Richard E.
Anybody out there handling/processing/sectioning mouse tissue containing mouse prions, if so, what safety precautions do you take?. Thanks Richard Edwards

[Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Silverman, Jeffrey
Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user

Re: [Histonet] Work Load Units

2010-02-23 Thread Rene J Buesa
What do you exactly mean by Work Load Units? You should know how many cases and blocks you process by day, week or year, and you also know how many hours your staff uses to complete the tasks derived from those cases and blocks. The total work completed between the staff members completing them

Re: [Histonet] PPE's embedding and cutting

2010-02-23 Thread Rene J Buesa
No! Neither to wear gloves when sectioning. Both are unwarranted requirements that interfere with the histotech's productivity. René J. --- On Mon, 2/22/10, rick.garnh...@memorialhealthsystem.com rick.garnh...@memorialhealthsystem.com wrote: From: rick.garnh...@memorialhealthsystem.com

Re: [Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Pamela Marcum
Thank you Jeffery.  I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause.  Is this something NSH might decide to help with?  It may be time to bombard them (CAP)

RE: [Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Mike Pence
The way I read this is if I don't use my cryostat 7 days a week, 365 days a year, then I don't use it daily and therefore I will set my own routine decontamination schedule. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Rene J Buesa
Please remember that from time to time CAP inspectors sit to ponder what something new can we add to our list so it appears that we are not using always the same old one?. And from time to time the come up with requirements like this. You will never change them and the requirements will keep

RE: [Histonet] Work Load Units

2010-02-23 Thread Weems, Joyce
Unfortunately, many facilities are only counting billable tests and all the recuts, sendouts, and work done pulling cases for conferences cannot be counted. It is a nightmare that needs to be addressed!! My 2 cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree

[Histonet] (no subject)

2010-02-23 Thread Madeline Gi
madelin...@yahoo.com   Madeline Rotger Milanese H.T. 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Jesus Ellin
To me the key word in here is interval. If a regularly scheduled interval is set, this could be daily , weekly, monthly or quarterly. Obviously there are going to be the times when a contaminant is there and the machine has to be brought down, but also I do not see the mention of the UV

[Histonet] seeking histology position - Ron Martin

2010-02-23 Thread Durden, Kelley
Go to https://jobs.ufl.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1266864723194 You'll have to create a user ID and login. UF will have a posting in the next week or so. ASCP license desired, FL DOH license is a plus. Also the Gainesville Veterans hospital has a position available -

RE: [Histonet] New cryostat decontamination CAP statndard

2010-02-23 Thread Silverman, Jeffrey
Folks: Please read the checklist notes carefully- I don't think for most of us this is open to interpretation. The standard note says: MUST be defrosted and decontaminated with a tuberculocidal WEEKLY for machines used daily. It's not open to interpretation if you do frozens daily. Tiny

Fwd: Re: [Histonet] Bone tissue

2010-02-23 Thread John Kiernan
Just for the record, Mollifex isn't alkaline. It is probably either Baker's 1941 mixture (see below) or the following (parts by volume): 95% ethanol 952 Glycerol 32 Acetone 80 Liquid phenol 8 - - - Liquid phenol is probably liquefied phenol, USP, which contains about 10%

RE: [Histonet] DRGs histogel

2010-02-23 Thread Karen Doty
I think dehydration during processing can effect the outcome with histogel. I use it frequently to help orient small samples with very good results almost always. One time, I was trying to arrange several samples in the same block and the histogel got too cold and set up when I was

RE: [Histonet] Double labeling with antibodies that need different fixatives

2010-02-23 Thread Andrea T. Hooper
Hi Phebe,   In my experience that CD31 clone you refer to doesn't work well mainly b/c of paraffin embedding -  in combination with PFA/formalin fixation. On PFA/formalin fixed frozen samples it works just fine with a trypsin antigen retrieval step. I find it's the paraffin that is the real

[Histonet] QA Report

2010-02-23 Thread Behnaz Sohrab
I was wondering if any one has special form that they provide to QA committee meeting in their hospital? This separate from all qc done in the lab!. Thanks,behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Any travel positions available?

2010-02-23 Thread Leah Cox
Has anyone heard of any travel or contract positions? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica Knife Holder CM1510

2010-02-23 Thread Andrew Burgeson
If anyone is in need of a CM 1510 leica cryostat knife holder for low profile blades, please contact me. Willing to part with this still functional item at a reasonable cost. FOR LOW PROFILE BLADES. Good price for back-up or replacement for Mohs or other enterprise. Not an easy item to find used.