Fw: [Histonet] frozen sections of cartilage

Hi Guys, I never received a response and I was hoping that someone would have some tips.  We are embedding and sectioning rat and mouse knee cartilage and I am having trouble keeping it on the slides.  Even when I do my IHC by hand, the sections are still lifting off.  Any suggestions? Kim  

[Histonet] Pathologist Assistant Jobs

Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other

[Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin?

Hello Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot

[Histonet] CD31 in rats

Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CD31 in rats

Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each)

[Histonet] CD 31 on Pig

Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD 31 on Pig

We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin sections (HIER with citrate buffer pH 6.0 required). It works on pig and rat tissue. Alexandra 2010/3/1 Reuel Cornelia reuel.corne...@tsrh.org Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank

[Histonet] IgG4

Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab

[Histonet] RE: Histonet Digest, Vol 76, Issue 1

I recall may years ago using the alcohol burner to heat my forceps and I was startled when the ice I had my blox on broke away from the pan and made a loud sound in an otherwise very quiet lab. I knocked over the alcohol burner and started a major lab fire. I used the extinguisher and all was

[Histonet] Herpes Simplex Virus Types 1 2 for IHC

Good afternoon, Does anyone have a nice HSV I II concentrate antibody for IHC on FFPE that they use. Biocare recently discontinued their concentrate and we don't do enough of these to warrant buying a predilute. We'd like a cocktail of the 2 types. Thanks, Linda A. Sebree University of

[Histonet] Job opening

Hi Histo World, We have a Histologist position available. Seeking someone self motivated, personable and team oriented! Anyone interested please email me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850)

[Histonet] Ab for Cartilage

Dear Histonetters, One of my colleagues is interested in demonstrating a marker for terminal chondrocyte differentiation in rat bone. It would be very helpful for her to know what antibodies others have used successfully for IHC. Thank you in advance for your responses. Joe Tamasi Senior

[Histonet] HSV I II

Thanks to all who responded to my inquiry about HSV III however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree

[Histonet] inquiry about Arthur Bolles Lee

Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information

[Histonet] Microwave processing and IHCs

Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we

Re: RE: [Histonet] CD31 in rats

Hi, we use Abcam mouse-anti CD31 also acetone fixed frozen sections. pretty well. 2010-03-02 TF 发件人： Hernandez, Anna 发送时间： 2010-03-02 09:50:38 收件人： Annette Featherstone; histonet@lists.utsouthwestern.edu 抄送： 主题： RE: [Histonet] CD31 in rats Hello Annette, We use Purified Mouse