[Histonet] Hello everyone, I am new to this forum

Hello everyone, I want to introduce myself to all the histotechs here. I have visited this forum before but I finally subscribed today. My name is Valerie, but please call me Val. I am an histotechnologist living in Puerto Rico. I got interested in the field of histology in 2008. I always loved

RE: [Histonet] Block patient IDs

The new derm path lab I am associated with built their own computerized specimen tracking system and I am pretty sure that the same multi unique identifiers that are printed on the slides are printed on the blocks. They have leica slide and block printers connected to accessioning and grossing by

RE: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody

Is the Perma Red for hrp? If you switch to an alk.phos detection system you could use fast red which is much redder than AEC. Dako sells ap/red detection systems, but I prefer to use Vector Red for AP. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045

[Histonet] Her-2 Controls

Morning All, My chief pathologist has asked the following question. For all of those doing Her-2 Neu staining for scoring with the Vias system. How many control slides do you cut in advance and how long are they viable if kept refrigerated? Thanks in advance, Rae Ann Staskiewicz

[Histonet] Histonet

dear Val, first my english is not good. I can understandbut the grammar! My way is similar- after studies I wanted to work in microbiologie. But I´ve got a job in Pathologie. First time I was alone in the laboratorie.I had to manage the routine...cutting,staining,preparation and so

Re: [Histonet] IHC on 3 year fixed tissue?

I have to imagine that you are doing the HIER in the FFPE sections? If you have used autoclave unsuccessfully it seems that the epitopes are so cross-linked that they do not respond to the treatment. I would try a piece of fixed tissue before processing, cut it in a very thin slice (about 2 mm

RE: [Histonet] IHC on 3 year fixed tissue?

Hey there. I have done IHC on mouse placentae that have been fixed in NBF for over 2 years and it took me 5-10 minutes of ProK (10 ug/ml in PBS) followed by 60 minutes of HIER in 10 mM citrate buffer (made sure PBS was at pH 6) in a veggie steamer. I had previously done this IHC in a

[Histonet] in situ hybridization histochemistry question about radioisotope

I have routinely seen that we obtain slightly different results (like 10-20% variance) in using 35S dATP for TdT-catalyzed tailing reactions of oligonucleotides from month to month as we use different batches (made fresh monthly) of the 35S dATP. But on a few occasions over the years we have