Re: [Histonet] number of slides

Here is our basic microtomy protocol: BMT - 3 HEs, each has a ribbon, plus PAS, Retic, Perls Liver bx - 3 HEs, each has a ribbon plus Retic, Trichrome, Perls, Renal bx - 4 HEs, each has a short ribbon plus PAS, PMS, trichrome - on slides with gloms Breast bx - 3 HEs, each has a ribbon Derm bx - 4

[Histonet] LOOKING FOR IHC POSITION

 Hi,     I am an HTL(ASCP) certified Histotechnologist with many years of experience. I am looking for a new position as IHC Specialist/IHC Supervisor/IHC Tech.     Open to relocation.   Isaac. ___ Histonet mailing list

Re: [Histonet] H E QC

Hi there, HE QC should be carry out on every slides that are stained, if you are checking for the staining intensity, this will be carried out using a control slide on new batch of staining solution, commercial or house made. Batch number should be recorded, and slides labelled with batch

[Histonet] Symphony

I was wondering who out there in Histo Land is using or has used the Ventana Symphony HE stainer and what their experiences have been. Thanks in advance!!   Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services   Tele (252)-830-6866 (800)-284-0672 Cell  (252)-943-9527 Fax 

[Histonet] Job Opportunity

We have a great full time permanent opportunity for an experienced histotech at Abbott Laboratories in Abbott Park, IL. We are about 40 miles north of Chicago in Lake County. Please go to www.Abbott.com if you are interested in applying for this position. Additionally, if you would like to

[Histonet] A.O TP8000 Processor

Hi - I'm cleaning out my office and found the procedure manual, timing disc and notes for the old A/O TP8000 Processor.If for some reason someone would like these I will mail them to you. Otherwise they are going in the trash. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist

Re: [Histonet] Silver Lips and Fingers

Beauty mark - like Cindy Crawford has. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and

[Histonet] Troubleshooting IHC

Hi all, I've recently run into a problem troubleshooting IHC on mouse bones. I am using the tyramide amplification system using a goat primary, anti-goat biotin, strepavidin-HRP, biotinyl tyramide, followed by another strepavidin-HRP and then DAB+ from Dako. When I use an isotype goat IgG, I get

[Histonet] Benchmark Ultra

Hello, We currently use a Benchmark Ultra and it is worth several XTs. There are some features about it that you should know about before you start a run. What we currently do in our lab is load all of the antibodies we are likely to run every day. This can be troublesome if you run the

[Histonet] Re: chick embryo frozen sections

hello, will some one please give me pointers on successful methods used for cryosectioning bursa esophagus from approx. 21 day chick embryos (such as recommended temp. micron thickness, for yielding the best sections)? Thanks! Atoska ___ Histonet

[Histonet] Re: Acetone Fixation

hello, if any of you have acetone fixation incorporated into you frozen section H E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples

[Histonet] RE: coverslipper

It depends on your volume and how long you maintain your slides. Because you are a children's hospital you keep them longer. The Leica brand is very troublesome in high volume situations. I personally prefer Sakura tape for the speed and fast drying time. -Original Message- From:

Re: [Histonet] coverslipper

We've had our Sakura film coverslipper since '94 and love it. The goodsaves us tech time coverslipping for hours each day, plus you can file the slides right away. The bad.the docs prefer the bone marrow smears to be coverslipped by hand. We have about 12 bone marrows a

[Histonet] Re: Acetone Fixation

What is your goal? A routine HE or immuno'setc.?? Atoska Gentry gent...@auburn.edu 3/25/2010 12:21 PM hello, if any of you have acetone fixation incorporated into you frozen section H E staining protocol will you please advise me on adjustments necessary for routine staining protocol.

RE: [Histonet] coverslipper

Leica CV3050 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I

[Histonet] whole mount protocol

hi all i am trying to do an in situ hybridization in whole mount brains at P7 but is hard to get staining mostly due to permeabilization issues since is 7 days postnatal brain. I would really appreciate to receive any suggestion or protocol to help me in this issue. Thanks a lot in

[Histonet] RE: coverslipper

Thanks a lot to all of you answered me. I was surprise nobody mentioned coverslipper from DAKO. Are any of you have any experience with DAKO's coverslipper? Again Thanks to all, Naira Subject: coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you

[Histonet] Re: Histonet Digest, Vol 76, Issue 38

On 3/25/10 1:04 PM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit

Re: [Histonet] coverslipper

We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if

RE: [Histonet] coverslipper

I purchased a used Hacker RCM-3660 glass coverslipper several years ago. It was missing some parts and the nice people from Hacker supplied me with the needed parts. This work horse has been running 7 days a week for the past several years and has never given us any problems. Great machine and

RE: [Histonet] coverslipper

Is this coverslipper now produced by Medite -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Thursday, March 25, 2010 2:40 PM To: malbena...@gmail.com; 'Lynette Pavelich' Cc:

[Histonet] Re: Troubleshooting IHC

Yes. I block in 3% H2O2, followed by protein block (it's a mysterious buffer called TNB that comes with the tyramide amplification kit), and then avidin/biotin. Adam On Thu, Mar 25, 2010 at 2:25 PM, Margaryan, Naira nmargar...@childrensmemorial.org wrote: Hi Adam, How do you block? I

[Histonet] Direction for TP 800

Hi - Got a taker for the directions. Who know? Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720

[Histonet] primate brain

I have some primate brain blocks to do. When I section the blocks the sections look nice. When I put the ribbon on the water bath( temp is 47) the tissue is wrinkled around the edges. No amount of time on the water makes this better. Ideas to solve this problem would be appreciated.

Re: [Histonet] primate brain

At what thickness do you cut your sections ? For Human brain we cut section at room temperature moist in Molifex and cut sections at 7 µm for standard HE/ 14 µm for LFB. Not sure what is the melting point of the wax your use, but if it is around 57 oC you can safely raise the temperature of