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Dear Histonet list manager
Is they anyway you could filter/block spam such as this one from be
sent out to the Histonet list.
Cheers
Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London
... Smile it confuses people ...
Begin forwarded
Pretty much any problems I have had with the Excelsior and I have been using
one for 4 years now is with Pathologists loading it incorrectly. I use reagent
alcohol in it all the time, with no problems. I prefer using xylene in my
processor. Every single time I have switched and started
There is about three brands of paraplast and they all cut differently. In one
of my labs I use paraplast plus and it ribbons very well however the blocks
have to be colder then if you are using TissuePrep from Fisher.
Leica may have sent you one of the other types of paraplast. (paraplast,
At one point we looked at getting an Excelsior and I spoke with our sales rep
and he said all the reagents we use in our processor now are absolutely fine.
He mentioned nothing about not being able to use Reagent alcohols or xylene
substitutes. Hope this helps - have a happy histo day!
Histonetters,
It is not too late to join us for the 2010 TSH meeting in Houston Texas April
23-25, 2010.
The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors
and Attendees.
Workshops begin Friday April 23, 2010 at 8:00am.
There is still plenty of time to
I am looking for help solving a lab mystery. The cassettes in our lab are
labeled with an SP Securline Marker II and then processed in a Sakura VIP
processor. Two weeks ago the entire batch came out labeled much more lightly
than usual, some to the point of where the label was completely
From June-15, 2009 CAP checklist
ANP.22997 Phase I N/A YES NO
If the laboratory performs HER2 testing (HER2 protein over-expression by
immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization
[e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented
I had similar issues with all of the marking pens out there. We finally
switched to Tissue-Tek Marking pencils #4160 and have not had a problem since.
Plus they never dry out!
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi there,
I would suggest to use a pencil rather than a marker pen to label
cassettes when processing cassette with the Sakura VIP , my lab had a
number of the so called solvent proof pen on trial and have yet to
find one that survive processing.
Malika Benatti BSc MIBMS
Specialist
YES! That is why I use pencil only. I had a processor full of blank/ unlabeled
cassettes because the lot# of the pens was bad. There is no way of knowing if
the ink is reagent proof until a disaster like you have occurs. You don't
always know if the ink lot passed its QC with absolute
Hi,
Tried post this the other day but I didn't see it go up. Can you make sure it
gets on the mailing list. Thanks! -Sam
Hi All,
I am trying to do IHC staining on FFPE mouse and human tissue to look for
autophagy.
Can anyone a recommend a good antibody which recognizes human or mouse LC3? In
Hello fellow Histonetters! I'm looking for cryostat machine model IEC-CTD.
They're the old ice cream box type used for frozen section, in case you have
this park on your storage areas and not using it, I'm very much willing to
purchase it regardless of its condition.
You can call me at
I have to agree. They have never failed and work forever.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Wednesday, March 31, 2010 11:30 AM
To: histonet@lists.utsouthwestern.edu
The only consistent lab pen I have used that will hold up under decal,
processing with various alcohol, xylene, hemo de is the KP marker plus. I am
sure there are several vendors, I use Mercedes.
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
For placentas, you will find that you get consistently good sections if you
gross the placenta fresh, take the samples you will want from the cord, disc
and make a membrane role (which is easily done if they are not fixed). Fix
your samples overnight and trim for blocks the next day. For the
I agree that these pens are excellent.
Many years ago at the National Naval Medical center in
Bethesda, MD we had an entire run of cassettes marked with
Xylene-proof markers come out of the processor with all
the ink dissolved and NOTHING on the cassettes!
Fortunately, we had everything in order
Hi there,
I was wondering what your hospital/centre's pathology policies indicate in
terms of the time you retain prosthetic valve specimens? Months? Years?
thanks for your feedback,
Anne Marie
***CONFIDENTIALITY NOTICE***
This electronic message and any attachments are intended only
I agree as well! - I use them to mark everything, even things that don't
get treated with chemicals.
One note: they work best if you mark hard non-porous surfaces (like the
plastic cassettes) the day before to give the ink time to bind with the
material.
Regards,
Merced
--On Wednesday,
I use nothing but SurgiPath (now LEICA) Blue Ribbon
Paraffin.
I run dermpath labs and this stuff is formulated for optimal
skin sectioning.
Highly recommend ithavent used anything else for 13
years.
AB
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Histonet mailing list
We photograph and retain only if requested. If not requested, we discard with
the regular specimens usually at least a month. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original
Hello,
Does anyone use MM24 mounting media from surgipath? We use cytoseal 60 but I
can get the MM24 at a lower price. If anyone has used this, could you tell me
whether you were satisfied with the product or not? Thanks so much.
Brandi Higgins
I use this and have had no problem with it.
Roberta
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
tigger...@aol.com
Sent: Wednesday, March 31, 2010 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject:
Denise, Note#16 is a guy Named Felton Nails , I have done business with
him in the past he at Children hospital in Texas, try calling him and
see if you would try our paraffin, You can mention my name and let him
know I said hello
John
-Original Message-
From:
We sometimes have problems with the stain if we use positive slides.
Margaret
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
We use it both for manual and automated coverslipping with great results
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We had the exact same problem that just started out of no where. One night
I came in really late to do a post and went through a department that
backed up to ours. Floor care was in there stripping the floors. The
cryostat was off again when I went in the room. The next day we used that
plug for
Anne Marie (where? Perhaps in Canada, which is going to have different
rules from the USA) was wondering what your hospital/centre's
pathology policies indicate in terms of the time you retain prosthetic
valve specimens? Months? Years?
I don't know of any specific policies. I've had a Carbomedics
Hi Margaret,
What problem are you experiencing ?
Are you over impregnated or under impregnated ?
At what thickness do you cut your Section ? Ideally 3 to 4 microns
How do you make up you Hexamine solution ? if you get contamination of
Hexamine solution make sure that glass ware is clean.
Try
We had the same problem with our Leica 3050S. It happened occasionally then
became more and more frequent. We went through a lot of the same hoops you have
gone through. When it shut down one day while I was cutting I knew it had to be
a machine malfunction. Leica replaced something that was
Anyone out there noticing problems with the Her2 antibody for Dako? Seems
real strong and deteriorating quickly. We order it in lots, so you can
imagine... Seems like every run is different. Same stainer, same titre ,
same tech. Tissue is from all over the country and world so we cannot
control
Is anyone concerned about the new (old) grossing personnel guidelines from CAP.
Many labs use people to process tissue. No more!
ANP.11610 Phase II
If individuals other than a pathologist or pathology resident assist in gross
examinations, do such individuals qualify as high complexity
just had a lively discussion at work. My take is that the only thing CAP
changed was that they combined the processing and grossing pieces
together again, which I don't know why they split them in the first place.
But you don't have the entire CAP note and many people miss this. The last
item
But above that after the education piece it says in Addition.
Jan, Omaha
-Original Message-
From: Joe Nocito [mailto:jnoc...@satx.rr.com]
Sent: Wednesday, March 31, 2010 4:08 PM
To: Mahoney,Janice A; Histonet
Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines
just had
Sally,
I added SubX to my acrymount since I have plenty of that in stock. It
worked!! And I am so glad I can use up what I have instead of having to
purchase a case of Sub X glue. My slides look clear and film free. I now am
more confident in passing these slides over to be read.
Thanks for
Are you interested in hearing about new job opportunities? I am a one of the
founders of a Healthcare Recruiting firm that specializes in placing Lab
Professionals. We work exclusively on permanent positions and have clients
across the country. We are completely free of charge to candidates
CAP is now saying no more gross processing of small things that are entirely
submitted- it's all gross examination now whether we mean straining
currettings into a cassette or dissecting a complex cancer resection. Dumb as
all get out if you ask me.
As for the three month training thing,
I just wanted to know if anybody who are working with Pig bone tissue fix in
10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval
pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit
for mouse monoclonal and rabbit polyclonal antibodies. I am
Very interesting! Coming out the nose is definitely bad for any work I have
done in the past - lungs get blown out, liver doesn't perfuse well and bone
marrow looks horrific. However, if you are working with PFA and doing a
post-fix anyway, you will probably be fine. If you are using GA and
Indeed,
One of the most EVIL products ever designed is the Permanent Lab
Marker by VWR. Talk about false advertising! We have researchers drop off
buckets of cassettes that they have labeled using these wonderful products.
Pencil is the ONLY acceptable means of labeling cassettes. It is just
Another option would be to invest in a cassette labeling machine, they
are few a available in the market, we use the Surgipath Cassette
Marker, they are pretty good as the ink fix with formalin and survive
processing.
Though ribbon need to keep refrigirated when not in use(overnight and
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