Dear All,
I have kind of funny problem.
I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct
labeling of my monoclonal antibody with flurochrome).
I am working on mouse liver frozen sections. When I did IHC the staining
looks like cytoplasm and if I do IF for the same protein
Hi Anil,
Perhaps you are having mouse-on-mouse issues with your IHC (DAB) and you are
seeing antibody cross-reactivity with mouse IgGs (you don't mention your IHC
detection method, so I could be wrong). You are probably not seeing this
cross-reactivity with the IF method, because the labeling
hi if u use triton for permeablization in IHC
it sometimes cause the stain pattern to diffuse from nucleus to cytoplasm
it is common
-- Original --
From: Kim Merriamkmerriam2...@yahoo.com;
Date: Thu, Apr 15, 2010 07:38 PM
To: Thotakura, Anil
I agree with Kim, your cytoplasmic staining with hrp/dab is probably because
your anti mouse detection is reacting to endogenous Mouse IgG in the mouse
tissue you are using.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
Imhojust do it on your tissue and include a no primary control.
If the latter is clear, don't worry about MOM.
If it is not, then we can advise further.
You don't state what Ab/tissue/detection system you are using..
This would help a lot as protocols will vary according to the tissue
Do anyone have experience with IHC staining for CK-7 in mouse pancreas?
If so would you mind sharing that info. I have had limited success with
this
Amrith
Notice: This e-mail message, together with any attachments, contains
information of Merck Co., Inc. (One Merck Drive, Whitehouse Station,
You may define whatever QA monitors for grossing that you wish. These
are not CLIA mandated, but contribute to improved performance. When it
comes to the individuals doing the grossing, you need to understand that
this has been interpreted as a high complexity activity with strict CLIA
Dear Histonetters!
I am looking for commercially available paraffin sections of the prenatal
murine human fetuses. I would appreciate any information.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
___
Histonet mailing list
Hello all of you histologists in AZ,
I have been requested to gather data regarding current wage scales
for HT's and HTL's in AZ from a local facility. In recent years,
larger laboratories such as Mayo Clinic and now, MD Anderson have
expanded their services to the Phoenix metropolitan
For any of you in the Boston area, my boss will be speaking on barcoding.
Victor
Original Message
Subject:[PPMA] Barcoding in Boston!
Date: Thu, 15 Apr 2010 10:34:31 -0700 (Pacific Daylight Time)
From: Rodney Schmidt schmi...@u.washington.edu
Reply-To: A mutual
Moving into a new lab and have tested several processors. Need some info on
the peloris. Good and Bad. We demoed the machine for about 2 months and had
an issue with our biopsy tissue staining too light (HE). We could not get
this problem resolved - we were running our tissue thru the
Can someone please let me know the best technique(s) they currently use for
demonstrating TRAP and alkaline phosphatase for osteoblasts and osteoclasts in
formalin fixed paraffin sections or resin sections?
Thanks
Barry
___
Histonet mailing list
We use the Peloris and have had no big problems with it. Certainly not
that affect our stain, however, we do not use it xylene-free. Her may
lay the problem.
I think the FDA question should be answered by someone from Leica.
Mike
-Original Message-
From:
Please answer this we are looking at buying one next year!
Jesus A Ellin HT/PA ASCP
Department of Pathology/Histology
Yuma Regional Medical Center
2400 South Ave A
Yuma, AZ 85364 - 7170
( Office: (928) 336-1743
(Fax: (928) 336-7319
*Email: jel...@yumaregional.org
Hi Patsy,
I'd like to verify the retrieval temp? In a previous email you wrote 70dc
and here you wrote 80dc.
Thank you.
Regards,
Merced
--On Wednesday, April 14, 2010 10:54 AM -0600 Patsy Ruegg
pru...@ihctech.net wrote:
For all those looking for this info from me.
I use the serotech
Hi Histotechs,
I am a recruiter at a highly successful and well respected Healthcare
recruiting firm. I help place Histotechs into permanent positions across the
country and I wanted to see if you are interested in exploring other career
opportunities?
Hi Everyone,
I would like to hear (pros and cons) from labs that have this instrument or
have looked at it. We currently use 2 of the oldest Dako autostainers and are
in need of updating. Any feedback would be appreciated.
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
I am in need of the same information if possible.
Pam Marcum
UAMS
Little Rock AR
- Original Message -
From: Jean Taylor jtay...@meriter.com
To: ih...@googlegroups.com ih...@googlegroups.com,
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent:
I have upgraded to the new autostainer links. I have four of them. There are
many pros and many cons. If you have any specific questions I'd be happy to
answer.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585)
I have upgraded to the new autostainer links. I have four of them. There are
many pros and many cons.
Too many to list. If you have any specific questions I'd be happy to answer
them.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
20 matches
Mail list logo
