[Histonet] Neonatal Mouse Brain Atlas

Hi Histo-lovers! I am planning on doing some nissl staining on neonatal mouse brain, to identify the trigeminal ganglion. Does anyone have a nissl stained atlas of mouse brain @ P0 (newborn) ? Has anyone had experience with this before? If so, would you mind sharing any tips? -- Kevin

[Histonet] Chloroform Fume Monitoring

** Proprietary ** ** Reply Requested When Convenient ** Hi Histoneter, I was just wondering what kind Health and Safety of procedures do people use to monitor Chloroform Fume ? I was carrying an HS audit for occupational exposure Xylene / Glutaraldehyde / Formaldehyde/ Chloroforms fumes

[Histonet] CAP Checklist

Message: 10 Date: Tue, 20 Apr 2010 16:56:48 -0400 From: thisis...@aol.com Subject: [Histonet] CAP Checklist To: histonet@lists.utsouthwestern.edu Message-ID: 8ccaee3211e306a-1c94-1...@webmail-m053.sysops.aol.com Content-Type: text/plain; charset=us-ascii Can someone tell me where on the CAP

[Histonet] Charged Slides for IHC on Ventana

Message: 2 Date: Tue, 20 Apr 2010 10:27:57 -0700 From: sris...@mail.holyname.org Subject: [Histonet] (no subject) To: histonet-boun...@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Message-ID: of4a13f24e.175f330a-on8525770b.005f84cc-8825770b.006ff...@holyname.org Content-Type:

[Histonet] Histology in the news....

Along with the new CAP/ASCO ER/PR guidelines there was a front page article in the NY Times yesterday: Cancer Fight: Unclear Tests for New Drug. It details the issues with interpreting Her2 staining and the continuing issue with disparate results from different labs when doing ER, PR and Her2

[Histonet] trigeminal

Kevin, This article has a nice photo showing mouse trigeminal ganglia. Google images is your friend-- Mike Nature Protocols 2, - 152 - 160 (2007) Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity Sacha A Malin,

[Histonet] IHC Slides, Hotplate vs Oven

Tony's answer (62 degrees C for 25 mins) is the minimum to ensure section adheres to slide. We all have to stain slides that have been baked longer than 30 mins. However, especially for Her2, longer baking times can result in false negatives or at least diminished staining. The justification

[Histonet] Bone marrow trephine decalcification

I'm in a bit of trouble. I work in the south of Brazil (Porto Alegre) for some years and now in my hospital a lot of bone marrow biopsies are being performed (leukemia and lymphomas). With decalcification with strong acids (nitric) I'm getting very poor results with the immunohistochemistry.

RE: [Histonet] Bone marrow trephine decalcification

They have commercial rapid decals that are formic acid based that do not affect the immunohistochemistry. But it does affect some of the enzyme histochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585)

[Histonet] tape coverships

Hello all I have a question on tape coverslips. We received a couple tape coverslipped slides in for scanning and the tape is completely removed from the slide. I would normally just coverslip with glass but the tissue is on the tape coverslip and not on the slide. How can I re attach the

RE: [Histonet] tape coverships

Just put the slide in xylene and coverslip with the tape containing the tissue. The you could put a couple small drops of mounting media on for good measure. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] tape coverships

Thanks it worked Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message-

[Histonet] Toluidine blue

Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] ascp exam

Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message

RE: [Histonet] IHC Slides, Hotplate vs Oven

Eric, Some of my thoughts on your discussion questions: 1. How do you monitor the shortened baking time, especially when multiple racks of slides are cut throughout the day? We have timers set for when each rack goes into the oven. This might be an issue for labs with larger batches (we are a

[Histonet] Charged Slides

Hi, If cost is a problem for charged slides, you could always silanize them yourself using whatever slides you want. It takes some time, (time=money) but the slides themselves are cheaper. There is a great description with procedures here:

RE: [Histonet] Toluidine blue

Newcomer Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of zodia...@comcast.net Sent: Wed 4/21/2010 5:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello to all, I was wondering if anyone knows where to

RE: [Histonet] ascp exam

Are you kidding!?! I have an AA in Histotechnology and still didn't pass my (HTL) written on the first round. I did pass my slide review though, thank you very much. :) I passed on the second try, which included 3 years of OJT and tons of studying after graduating school. (BTW, I also have a