[Histonet] Campylobacter jejuni antibody?

Hello histonet, I need help with Campylobacter jejuni antibody, where can I purchase it? Protocol? Control slides? Any help would be very much appreciated! Thank you Chakib Histology supervisor HTL(ASCP) MIT-DCM ___ Histonet mailing list

[Histonet] Best charged slides for IHC

Has anybody done a comparison of charged slides, to see which ones are best for IHC staining - espcially when slides will be pretreated in a pressure cooker? -- Sally Price ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Tilapia eyes

Fish eyes tend to like different temperatures and water content during cutting. Chill the eyes well after facing off, then when cutting dip a brush in the warm water bath and paint the lens area, warming it up a little and moistening it at the same time. If the eyes are processed properly,

[Histonet] HT/HTL - Field Laboratory Application Specialist (Western US)

My name is Alicia Moore and I support the centralized staffing function within the Specialty Diagnostics Group of Thermo Fisher Scientific. Thermo Fisher Scientific Inc. (NYSE: TMO - News) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and

RE: [Histonet] Is anyone else having an issue with their paraplast?

I'm relieved that someone else is having the same problem. I was beginning to think it was my processor. I have already decided to switch paraffin. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] Hep C Antibody

Hi Histonet, Wondering if anyone has ever used a antibody for Hep C on formalin fixed paraffin embedded tissue? If so where did you get it. Thanks in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210

[Histonet] RELIA Special Job Alert for Immunohistochemistry Specialists 5/14/10

Hi Histonetters!! I hope everyone is getting ready for a great weekend! I have a few position that I want to tell you about. All of these position are full time and permanent and all of these clients offer competitive salaries, great benefits and relocation assistance. I am working with

RE: [Histonet] RE: AMT Cover

All, I just looked at the notorious AMT cover that people are up in arms about and I must disagree with the comments that were posted thus far. The techs here and myself are quite surprised by the reactions because it is on a medical catalogue and is far from being lewd or obscene. You could

RE: [Histonet] RE: AMT Cover

I second that! Josie Britton HT Keene, NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, May 14, 2010 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: RE:

[Histonet] large fibrous bone tissue

How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a

[Histonet] Grossing Technician Qualifications

Hi Histonetters: Are the guidelines for a tech who is strictly limited to grossing anatomic pathology specimens different than for those of a full time histotech? Could a bachelor degree'd person with the right course work qualify for this position? If so, does anyone have documentation of this?

[Histonet] (no subject)

Is anyone using Surgipath's Select Tech HE kit? I have a sample kit and I want to try it out on my Leica XL autostainer. I am looking for a protocol with times/dips etc. Thanks, Cheryl A. Miller HT ASCPcm Histology Supervisor Physicians Laboratory, P.C. 402 493 0403

[Histonet] traveling histotechs

I was wondering if anyone has any information on traveling histology technician companys. I wish to try the traveling tech world and do not know how to get started. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] available positions in Dallas

I have 2 full time positions available.  We have a newly created position for a histotech with experience for an 11:00 p.m to 7:30 a.m. shift.  We also have a position available at our private laboratory for a histotech with immuno experience.  If you would be interested in either of these

[Histonet] IHC marker for carbonic anhydrase 9

Dear all We are locking immuno marker for carbonic anhydrase 9 on p.section. Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Are Histotechs considered exempt employees?

Wages are totally region specific, in Colorado the cost of living is not as high as it is in CA for sure and many other places, plus since Colorado is a desirable place to live we usually don't have trouble getting people to come here to work, so it is a supply and demand issue. It keeps our

[Histonet] Prolonged FFPE slide and block storage

Hi All, We have a growing need for storing slides. They need to be stored in a way that they won't become oxidized and loose their antigenicity, since we use them for IHC. Any suggestions for inexpensive methods for prolonged storage of FFPE slides and blocks is greatly appreciated. Thanks!

[Histonet] Mounting Medium Issues

Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number

[Histonet] RE: Prolonged FFPE slide and block storage

I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original

[Histonet] IHC

Can you leave your primary antibody on slides overnight (or the w eekend) or will this screw things up? I don't want to be here another= couple of hours for secondary, etc. on a Friday!! Sarah Goebel, B.A., HT (ASCP) = iH= istotechnician XBiotech USA Inc.

Re: [Histonet] IHC

Only if the procedure calls for it. If not, then you can try holding the slides in buffer and refrigerate. Just be sure to allow the slides to come to room temperature before resuming. - Original Message - From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] IHC

I always leave my primary antibodies on overnight at 4C. I've seen some protocols for tricky antigens where people leave it on for days. I wouldn't leave it a room temperature though. Go home and enjoy your Friday, Adam On Fri, May 14, 2010 at 4:37 PM, sgoe...@xbiotech.com wrote: Can you

Re: [Histonet] Prolonged FFPE slide and block storage

Sam- Are you storing sectioned slides? What we do is to deparaffinize and coverslip sectioned but unstained slides.  I haven' had to go back, but I believe they will store that way a long time. Joe Saby, BA HT From: Perry, Samuel

Re: [Histonet] IHC

Overnight is surely fine but make sure at 4 deg C for two reasons. First you want to minimize any bacterial growth. Second you want to ensure it doesn't evaporate significantly which would change your antibody concentration. For that same reason make sure you humidify the chamber amply as well