RE: [Histonet] New CAP question ANP.22760

2010-06-17 Thread Ellen Yee
Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.)

Re: [Histonet] release of tissues

2010-06-17 Thread Karen Sly
Mary Sue, Our hospital regular releases gallstones to patients and/or their doctors. We make a record of it in our computer system as to where and to who the gallstones were sent/released to. Karen Sly BS Biology Pathology Department Central Michigan Community Hospital Mt Pleasant MI. Please

[Histonet] Immuno

2010-06-17 Thread Carol Fields
Hi All, Does anyone out there know a lab that is able to do a Human Herpes Virus, Type 6 immuno? Thanks for any help on this. We haven't been able to find a reference lab that does this. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@norths

[Histonet] release of tissues

2010-06-17 Thread Mary_Joy
Hi all, How would you handle a request from a surgeon, f= or release of gallstones or any tissue, to a patient? Assuming a 'Rele= ase of Tissue' request form, indicating the biohazard staus of the tissue,is signed by the patient, is this commom practice? Thank

RE: [Histonet] intervertebral disc

2010-06-17 Thread Jack Ratliff
Jennifer, What do you wish to accomplish histologically? Do you only wish to see the disc material? Do you care about the cranial and caudal vertebral bodies? Are you wanting to perform IHC? Please tell me a little more so that I can provide you with a more detailed options. I am assuming t

RE: [Histonet] Breast Specimen handling

2010-06-17 Thread Maria Katleba
Problem, if you store it in alcohol, you compromise the potential for IHC, and other tests. It somehow messes with the antibodies. But I am no expert Supposedly its why people switched to formalin fixation instead of alcoholic fixatives Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Que

RE: [Histonet] how to prevent foldings on femoral head cartilage tissue

2010-06-17 Thread Jack Ratliff
Reuel, Do the opposite and turn up the temp on the waterbath and let the section float out a little before the wax starts to break up. You can even gently use the forcepts to tease out any folds and this will definitely help to release any wrinkles in the cartilage. Then, make sure the sect

Re: [Histonet] Breast Specimen handling

2010-06-17 Thread BSullivan
Sara, Funny that this comes up now. I attended a meeting last week and this was one of the topics. It was suggested that the tissue be stored in 70% alcohol. I personally have not done this but I am going to start doing this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor S

RE: [Histonet] how to prevent foldings on femoral head cartilage tissue

2010-06-17 Thread Liz Chlipala
Sometimes placing them on a hot plate at about 60C will help get out the folds, the paraffin needs to melt and the sections need to turn clear then take it off the hot plate. If you leave it too long on the hotplate the articular cartilage may fold over on itself. Liz Elizabeth A. Chlipala, BS,

[Histonet] RE:New CAP question ANP.22760

2010-06-17 Thread Daryl Mikita
Hello, We are using our appropriate antibody control tissues run side by side to do the antibody and detection system lot tests. One using the old lot number of reagents and one with the new lot number of reagents. TTYL, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233

Re: [Histonet] alcohol monitoring

2010-06-17 Thread Cristi stephenson
Thank you all so much for the guidance!  I appreciate all of the input. Sincerely, Cristi From: Lee & Peggy Wenk To: Jeff Birkner ; Rene J Buesa ; Histo Net ; Cristi stephenson Sent: Wed, June 16, 2010 6:18:15 PM Subject: RE: [Histonet] alcohol monitoring Y

[Histonet] Breast Specimen handling

2010-06-17 Thread Sara Baldwin/mhhcc.org
Hi histonetters Was wondering if anyone saw the new gui= delines for breast Cancer specimen handling ? I am sure you have.&nbs= p; What my Pathologist is wondering is if you have a large specimen and do = not use all the tissue for processing what would the agent be

[Histonet] how to prevent foldings on femoral head cartilage tissue

2010-06-17 Thread Reuel Cornelia
Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still hav

RE: [Histonet] Thermo Slide Mate & Print Mate

2010-06-17 Thread Laurie Colbert
Mike, We have the Print Mate and 5 Slide Mates. We are not connected to our LIS - we use it as a stand alone process. Everything is set up very basic and the system works very well for us. Feel free to contact me offline. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 -Ori

[Histonet] Thermo Slide Mate & Print Mate

2010-06-17 Thread Mike Pence
Is anyone using the Thermo Slide Mate & Print Mate with CoPath as your AP LIS? I am trying to decide if I am going to look at this as a stand alone process or integrate it with my AP LIS. I would like to hear from those that use it both ways and if it is okay to contact you off line. Thanks,

RE: [Histonet] fume hood

2010-06-17 Thread Feher, Stephen
Hi Brandy, I have recently had the opportunity to build a Path lab from scratch. In the design we decided to completely separate the grossing area from the microtomy and IHC area of the lab. We built a "room within a room", made it negative pressure, installed 2 Thermo elevating grossing stations