Hi,
I am new to histology and have some very basic questions. I hope somebody out
there willing to help me.. I want to isolate tumours from mice and preserve it
for histology . I heard that there is different method on how best preserving
different organs/ tissue. Could any of you please
Carolina,
It will depend on what you want to do to the tissue after removal. For
Histology, placing the tissue in 10% phosphate buffered formalin is good
for morphology, special staains, immunohistochemistry and in situ
hybridisation. I have been able to extract RNA and DNA from formalin
fixed,
We have been having trouble with big breeze blowing our precious ribbons
out of our hands while cutting. We would like a door on the Histology
lab to cut down on the breeze of people walking by through the hall.
Our facilities want to find out what other people are doing to stop this
problem. We
We have several openings for our fast growing lab. We need Registrations
Associates as well. The open positions are: 1 Full time tech with EM
experience, 1 full time tech with IHC experience, 2 full time day shift
positions and 1 full time overnight position. Excellent opportunity for NYS
Hi Josie and Chris,
I've worked in labs with similar problems. Breezes are nice outdoors but
cause havoc with ribbons. If you can't get them to put in a door (which
you'll probably have to lock since folks will walk in, shut the door and
cause a breeze that way) then how about those fuzzy
For the ceiling vents, you could ask to have deflectors installed. The ones we
have are made of stainless, are slightly larger than the vent, and are
suspended about 6-8 below the opening.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Histoland,
For those of you who order your H-pylori/Giemsa controls: Who do you
use?
There were 2 companies that we were using for years, and all of a
sudden, there's no bugs in the tissue-many, many boxes were without
bugs! And they told my boss that the IHC controls are not 'guaranteed'
You have got to be kidding!! That's hysterical. So process a slim jim
and you have
Gram - and + controls. If you're serious I'm trying it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie
Britton
What a waste of a good Slim Jim.
Victor
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
A good ol' hot appendix works great. Not as good as a Slim Jim, tho.
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When I was doing histology, I would use a flexible end of a brush to roll the
ribbon end closest to the blade and that would secure it beautifully and no fly
aways.
Robyn Vazquez
From: histot...@imagesbyhopper.com
To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com;
Do you put the slim jims in formalin and then process them or just put them in
the processor?
Margaret
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Ditto, I use a small brush that I got from the craft store and prior to
picking up the ribbon I wet the brush and slip it under my ribbon end
closest to the blade and presto no flyaways.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Dear Frank,
Good luck with ridding your samples of autofluorescence. Fat is always very
brightly fluorescent and I suspect it might even be so without any aldehyde
fixation. First I wondered how successful you'd be using Sudan Black B, knowing
it is a fat stain, and knowing that it's been
I just found a partially eaten Slim Jim snack picture on wikipedia...
Is it Friday again? :-
Do you put the slim jims in formalin and then process them or just put them
in the processor?
Margaret
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Do slide warmers cost too much?
I don't know how much they cost, though, maybe an oven is cheaper.
Emily
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff
Tried the slim jim and all of my doctors did not like it. Don't waste your
time.
Connie G.
Date: Tue, 22 Jun 2010 10:16:14 -0400
From: cg...@marylandgeneral.org
To: jcbrit...@cheshire-med.com; dianar...@aol.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In
Tom:
As much as I agree with your acknowledgment that its seems a bit odd for
the CAP to have a blood-banker responding to AP-related issue, I'm actually
not surprised. The folks in the 'clinical' lab have been performing more
comprehensive and complex validation procedures for a very long
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