[Histonet] xylene substitute

2010-07-02 Thread Vanessa Avalos
I am taking a chance that this is still a working email!! I came across an old post on histonet regarding Xylene subs with a linear stainer. I was wondering if you made the switch, and if so could you share the brand and your H&E protocol. I have had some issues with Eosin bleeding, slides very eo

RE: [Histonet] BILLING QUESTION

2010-07-02 Thread Feher, Stephen
You can contract for services to another lab or hospital and depending upon the terms of the contract, you may be able to bill the patient. For example, you send all of your HER2's and ER/PR to another lab to be stained and quantified into a score. You may contract with the other lab for them to c

[Histonet] bat wing histology

2010-07-02 Thread Amos Brooks
Hi, I do hope you are looking at cross sections of the wing and not the flat. That would be very difficult indeed. For good cross sections I would try a "Swiss Roll". This is a way of demonstrating a large amount of cross sectional area in small space. Take the membrane and fix it by submersion

[Histonet] Looking for MIN6 cell line

2010-07-02 Thread Debra Baluch
Does anyone know where I can obtain the MIN6 beta cell line derived from mouse pancreas? They are listed and used in many papers but do not list the source. I have tried ATCC and suggested cell banks in ECACC (England), DSMZ (Germany), and JCRB (Japan) but they are not listed. Thanks, Page

[Histonet] Thomas Crowell is out of the office.

2010-07-02 Thread thomas . crowell
I will be out of the office starting 07/02/2010 and will not return until 07/13/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu htt

[Histonet] Re: control tissue for Hall's bile stain

2010-07-02 Thread Robert Richmond
Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long ti

RE: [Histonet] BILLING QUESTION

2010-07-02 Thread Cynthia Pyse
We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.ed

RE: [Histonet] ER and PR validation

2010-07-02 Thread JMyers1
This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe -- Message: 12 D

[Histonet] BILLING QUESTION

2010-07-02 Thread Sara Baldwin/mhhcc.org
Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald..

Re: [Histonet] massons question

2010-07-02 Thread Bryan Llewellyn
SuSa has a high mercuric chloride content, and tissues fixed in high mercury fixatives do not require secondary fixation in picric acid (Bouin). The mercuric chloride fixes in a manner which improves staining with acid dyes. For Masson's trichrome on tissues fixed with simple formalin variants

[Histonet] ER and PR validation

2010-07-02 Thread Webb, Dorothy L
In a recent article written by 4 pathologists, including Dr. Elizabeth Hammon and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are

Re: [Histonet] massons question

2010-07-02 Thread BSullivan
Bouin's is used as a mordant in this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 mohamed abd el

[Histonet] massons question

2010-07-02 Thread mohamed abd el razik
hi all i'm going to stain some slides of small intestin which were fixed in susa. i'm going to stain it by Massons trichrome. i have read that slides should be secondry fixed in Bouin's or saturated picric solution.   does it mean fixation of processed tissue after cutting with microtom?? how lo