Wow that is going to be a tuff one, back in the day I tried to cut Petris
pyramid from the inter ear and was told it was the densest/hardest bone in
the body and it sure seemed to be. Even decalcified we had to embed it in
plastic and use permanent tungsten carbide D profile knives to cut any
Oh my, I agree with Liz, your samples are way too thick and your process is
way too short. Even for 3-4mm thickness bone I fix for 24-72 hours and then
decal in 5% formic acid usually overnight on a platform shaker. I assume
RDO decals a lot faster but not that fast, I do not use RDO because I
Yep that is what I do, I use the plastic containers I get salad or other
stuff in I would throw away, fill it and freeze it, I add some water to it
and put the blocks on the frozen tray and they get cold and hydrated at the
same time.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd.
I do not bake my unstained slides for IHC controls and store them at 4dc. I
bake them just before use. They do last longer this way but Richard is
right, there is no telling because it all depends on the fixation, target
protein stability and storage conditions. It is always a good idea to cut
