[Histonet] Difference between Protease and Proteinase-K?

Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the

[Histonet] Phospho-Histone 3 (SER10) antibody

I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated.

Re: [Histonet] Difference between Protease and Proteinase-K?

Hello Jennifer I'm not sure what platform you are using, but from my experience, I obtain beautiful results for CD31 in canine and feline tissue, but the protocols for each are quite different. For CD31 in canine tissue I use a tris based buffer and for feline I use protease. I know this

RE: [Histonet] Phospho-Histone 3 (SER10) antibody

Martha, We have good success with Millipore 06-570 using citrate HIER - also with the (pSer28) Histone H3 from Epitomics (#1329). Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803

[Histonet] Great Histotech Job in South Carolina

Dear Histonet Members, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career

[Histonet] RE: air dry vs heat dry heat

Hello, Thanks for all the replies. I find them helpful and it may help to resolve some of our lab 'discussions'. Thanks again, Jeff ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] air dry or heat dry

Hello Jeff, Let's see you are sectioning your (fixed) rat brain on the cryostat at 10ums. I'm assuming they are mounted on standard size superfrost plus slides since they are fixed sections even if they were not fixed - after sectioning why not air-dry these sections standing up under a

[Histonet] BOND III VS ULTRA BENCH MARK

I need help about these immunostainers, Does anybody has idea about the best out of the two? I want to know the ADVANTAGES AND DISADVANTAGES of each.Thanks GUY Tunde Ajibade  BS HTL(ASCP)QIHC Pathology lab Newark Beth Israel Medical Center. Newark NJ 07111