Toluidine blue stains striated muscle nicely.
Regards
Birgitta Stephenson University of Queensland Microscopy Research Lab
On Tue, 17 Aug 2010 17:18:18 -0400, Sherwood, Margaret
msherw...@partners.org said:
Masson Trichrome stains collagen and muscle.
-Original Message-
From:
This is a learned histology skill, but there are some devices which can be used
to align different microtomes available, and you can test your adjustments with
blank blocks. Also newer microtomes have a device on the block holder that
will align the position in the X-Y axis in the zero
A while back I remember someone suggestion something like a right angle
device that carpenters use. It's basically just a piece of metal that is a
right angle triangle that you put up against the chuck and on the knife
mount. Then you align the chuck so it is a a right angle to the knife
mount.
Yes, these used to be available from Newcomer supply, but I think they are now
de-funked. Try a google search perhaps?
Joelle
Date: Wed, 18 Aug 2010 07:00:23 -0400
From: pat...@gmail.com
To: wdesalvo@hotmail.com
Subject: Re: [Histonet] Microtome alignment
CC:
There is an immunofluorescence technique that uses wheat germ agglutinin
(WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the
cell periphery, leaving you with beautiful traces of the outside of each
cell. The cell area is then simple to analyze with ImageJ or any other
software
Reminder... these 'please send money' or 'please send your bank acct
information so that I can deposit money into your acct' pleas are ALWAYS
scams. They have been for years. If a trusted 'friend' was truly stranded
in a foreign country, they'd borrow a phone and call home. If a long lost
Does anyone have experience with the rabbit monoclonal anti-thymidine
kinase 1 (clone EPR3193) on FFPE tissues? i.e. antigen retrieval
necessary or not?
Thanks as always,
Brett
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
Andrea - Wow this looks great. We do a lot of cardiomyocyte staining and
studying and this could be helpful. Thanks for sharing!
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY
Would you know a techniqe to confirm cell viability in paraffin embedded
tissue? Propidium and methylene blue staining are used in cell culturing to
detect cell viability. Is there similar technique for detecting cell viability
in tissue?
This question was asked by our research director, Can
Cornelia:
After a deadly fixation, followed by a complete dehydration, clearing and
paraffin wax infiltration, the only potentially surviving entities are prions.
No cell will be viable after this treatment and if you get some positive
results of viability with what ever test you use, I caution
Hey Ruel
Once we are done with them and they are all nicely embedded in paraffin
wax...the cells are not viable...they are DEAD
Annieinarabia
Empower your Business with BlackBerry® and Mobile Solutions from Etisalat
-Original Message-
From: Reuel Cornelia reuel.corne...@tsrh.org
Sender:
MPath Search Partners has been retained to search for a
Histotechnologist/Histotechnician who is available for a vacation coverage
shift in Marietta, GA.
Position Title: Histotechnologist/Histotechnician
Shift: September 6th-10th, 2010. That’s Monday-Friday 6-8 hour days with
lunch
I assume you are referring to a small carpenter's square. That will only allow
you to square up the chuck from top to bottom. It would not work for left and
right adjustments. Marketlab sells an alignment tool that they claim will work
for multiple microtomes. I have not tried it though.
We recently purchased the new alignment tool from Source medical products that
works on any microtome as it uses a leveling bubble. It is round, so it makes
certain all is level from any direction. Works well and has helped us out. I
have staff use it daily as it takes minimal time. Only
Hi Saro and others,
Our protocol is attached. It is very simple.
The only trick is to remember that if you are using this for heart tissue,
each section will likely contain cardiomyocytes sectioned at various
angles. To accurately measure cell size/diameter, you will want to measure
only cells
For those of you who have experience with the Bond or Benchmark XT; what do
you like/dislike about them? We're looking at advanced stainers as well. Any
reoccurring problems you could do without? You don't really hear about those
when meeting with company reps.
Thank you in advance for any
I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.
Joel
--
Joel Reichensperger
Researcher II
Southern Illinois
Blood vessels in tissue. Here is a website that I use for p= ositive
control list.
[1]http://www.pantomics.com/support/IHCcontr= oltissuelist.htm
There are tons of lists out t= here, this is just one.
Sarah Goebel, B.A., HT (ASCP)
H= istotechnician
XBiotech USA Inc.
Just about any tissue should work. It stains for Hematopoietic
stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic
fibroblasts
We use a sausage style control here with about 30 different types of tissue.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Hello ,
I need 4 good plastic containers, where you fill with xilene,alcool... for
the VENTANA RENAISSANCE .
Anyone could sell me ?
Regards
Ricky
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Histonet mailing list
Joel,
A section of skin will be a fine CD34 control. All collagenous connective
tissue, including the dermis, are rich in CD34+ dendritic interstitial
fibroblasts as well as CD34 positive endothelium in the resident vessels.
Actually, in my lab we use a section of fallopian tube from tubal
Dear Histonet-ers,
We currently use tonsil for a positive control tissue for IgG, A and M when we
do direct immunofluorescence on skin specimens. We look for occasional plasma
cells that stain positive in the cytoplasm, but of course there is no positive
ID of what is staining, since the
Hi Ricky,
We may have what you need, we have multiple sizes of containers which we can
either send empty or can fill for you whichever you prefer. Check out the
site if you would like at www.sensorhealth.com
Hope that helps.
Adam Harris
Sales Associate
Sensor Health Inc.
110-6 Turnbull Crt.
What stain platform are you using?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, August 18, 2010 11:04 AM
To: histonet
Subject: [Histonet] Fontana
Hello everone,
We
Glad you're not stuck, Jerry. This is why I act like an old codger and refuse
to get a facebook,other social networking sites, etc. account. What's the deal
with farmville anyway? :)
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jerry
I really have nothing bad to say about the benchmark. We love ours; it has
freed us from having to nurse the IHC bench. You load it and walk away. If
there was a down side I would say that the antibodies are a bit on the higher
price side. You can find cheaper antibodies but then you have to
Hi,
My favorite tool works on all microtomes and is priced perfectly. The
knobs on the block holder move the block so when it looks like you are going
to cut too much of a block you turn them and, voila, a perfectly aligned
microtome.
Amos
Message: 2
Date: Tue, 17 Aug 2010 14:16:26 -0500
Hi Emmanuel, I am a Canadian trained and certified tech working here in
the US. You will have to contact the CSMLS (Canadian Society of Medical
Laboratory Sciences) to find out were to send your educational info to
apply for Canadian certification. You should be able to do a google
search and
--
What we do is color code out microtomes (red, blue, green etc.) and each
station has a matching marker. The tech will put a colored stripe down
the side of all the blocks that he/she cut. If we have to pull a block
out of the file for recuts, we know exactly
hI,
Placenta is a very good control for CD34,try it you will like it.
Tunde
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