[Histonet] new lab design

Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision:

[Histonet] Histotechs Needed!!

Good Morning, Our team here at Personify has recently been partnered with a World Leader in Cancer Diagnostics who is going through a large expansion due to their recent and continued success in the Immunohistochemistry market. Due to this expansion we are currently seeking Histotechs who

[Histonet] re: new lab design

Congratulations on your move to a new building and the opportunity to have a fresh start in a new lab. The first thing I would suggest is to NOT blindly depend on a laboratory design firm to design your lab. Consider the design and operation of your new lab like you would buying a new car.

RE: [Histonet] Flat Ice???

Thanks. I am going to try that ice rink: tip. :-) What I do to keep my blocks from sliding all over the place now is put a kimwipe on my wet ice. They can cool and I also like to hydrate my blocks a little. I feel it gives me a better looking section histologically. my 2 cents as well. ;o)

[Histonet] SOX-10

Happy Monday Histonetters, I'm wondering if anyone can share a protocol for running SOX-10 on the Ventana BenchmarkXT? (On FFPE tissue) Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged.

Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)

I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It

[Histonet] Napsin A

Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list

RE: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)

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RE: [Histonet] new lab design

Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -Original Message- From:

RE: [Histonet] new lab design

Thanks Tim! Steve -Original Message- From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] Sent: Monday, September 13, 2010 12:38 PM To: Feher, Stephen; Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design FYI, I have been to Steve's lab. They have a great layout. A lot of

RE: [Histonet] new lab design

FYI, I have been to Steve's lab. They have a great layout. A lot of time and effort was spent in the design of it and it shows. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -Original Message- From:

RE: [Histonet] new lab design

Steve's workshop at NSH last year was a good intro to lab design. The most significant take-away was that they did many, many modifications on paper before they ever did anything for real...and all with LEAN principle driving the design. Tim Morken Supervisor, Histology, IPOX UCSF Medical

RE: [Histonet] Napsin A

We are using a Lung Adenocarcinoma as a daily control. Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained

FW: [Histonet] Liver Processing Question

-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, September 10, 2010 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver Processing Question Hello

RE: [Histonet] new lab design

We recently built our new facility from the ground up. Having worked in various histology labs, I knew what works and what doesn't work. Planning the lab around work-flow is crucial. I started the design by simply writing the name of each room I wanted constructed on a sticky-note. These were

Re: [Histonet] Napsin A

Hi Loralee, Thanks for your response. What did you use for the multi normal tissue block? Kidney? Also for the multi-tumor block did you just use tumors from organs that  you knew were negative for Napsin or did you use both positive and negative tumors? Thanks, Jessica

[Histonet] coverslip removal

I am using aqueous mounting media, faramount, for the first time. The slides I coverslipped several days ago look dry. Will soaking in water remove the coverslip? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007

Re: [Histonet] Napsin A

Hi Jessica, Our control for this antibody is normal lung, adenocarcinoma of the lung, and thyroid. We use this same block for other stains too (TTF-1, sufactant-A, EMA, etc.) but if I were going to make a control block specific for this antibody, I would want to include a squamous cell carcinoma

[Histonet] Re: Solvent Recycler

Timothy, We have the B/R 9700 ProCycler to recycle alcohol, xylene, and formalin. The Advantage must be a newer model. About 70 gal of alcohol, 40 gal of xylene, and 20 gal of formalin is recycled every 3 months. This recycler is easy to use and works well for us. As with any equipment, you

[Histonet] Form for validating slides on digital slide scanner

We are just getting started with our Digital slide scanner and I am looking for help. Anything you would be willing to share in terms of SOPs, Policies in regards to CAP and validation forms used when you are initially validation the machine would be helpful. Thanks, Mighnon Lashus, HT (ASCP)

[Histonet] Snap freezing using a Dry Shipper?

Hi Histonetters, I need some advice as my group is tasked to procure tissue from surgery sites off-campus. Logistics within those other hospitals do not allow a liquid nitrogen source, and my health and safety folks have asked us to consider using a Dry Shippers or cryoport for snap

[Histonet] Sakura X50 Microwave Tissue Processor

Thanks to everyone who gave some input on the X50, it helped a lot and has me thinking twice about that particular piece of equipment. Carlos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu