Hi,
This looks really interesting. Do you know if there is the same type of
path courses/workshop for rodents pathology?
Regards
Jeanne
Jeanne Estabel, PhD
Scientific Manager
MGP Histology Operations Manager
Wellcome Trust Sanger Institute
Cambridge UK
-Original Message-
From:
i think that histonet is a primary educational group for all levels and any
expert in our feild have asked these quistions one day befor and we should ask
freely without any shame .
i'm as begainner have learned alot from these little quistions.
and i asked befor for name of antibodies and its
Dear Histonetters,
Our pathologists are interested in rat heart valvular lesions. I am working on
trimming and sectioning methods to get uniform and reproducible morphology of
heart valves in paraffin sections. It seems that available literature dealing
with this is limited. Requesting your
We have the Leica Cassette Labeler. It works just fine. I have looked
at the Thermo Fisher Cassette Printer, which had a much smaller
footprint than the Leica. I would be tempted to go with the Thermo if I
have to replace my current labeler.
These are expensive instruments but worth having.
Thermo has a relatively low cost labeler for smaller quantities of
cassette printing.
The cost of the labeler versus the cost and potential for errors (hand
writing) needs to be considered when making this kind of decision. You
might want to do a study where you actually measure the time it
Good Morning,
We process about that many blocks per day also, and I would say YES it is worth
getting a cassette labeler. Leica/Surgipath has a small semi-automatic
cassette labeler that we are in the process of buying and we LOVE it. It will
print the accession# and the patient's name on the
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http
Allied Search Partners has been retained to search for a Histotechnician or
Histotechnologist who can remit their services to our client for 6-8 week
coverage. The physician is looking for a qualified candidate to work ASAP.
*Location of Laboratory:* Toledo, OH (local candidates please)
Hi Itai,
I am interested to hear if you've resolved this problem. We use the same
kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is
similar to the one you mentioned. I cannot get nuclei staining with this
method either. The nuclei are well stained (ie black) up to the
I do Weigert's staining for 10 minutes and use it for a week at the most. I,
too, have heard conflicting times about the stability of the Weigert's, but
have
the best results with using it no longer than a week.
Deanna Rhoads HT (ASCP)
From: Andrea Marion
In general, we found the original/classic Weigerts iron hematoxylin stain to
be weak and almost washed out of tissues after staining with Massons
Trichrome. We no longer use the original formula, but a more concentrated
modified formulation that is not differentiated out as much by the acidifed
My first reaction to the what is happening to our field, was WOW. It
seemed unkind to me, as if they original poster should not have asked these
questions. With further reading of the replies to this post, I am not so
sure it was an unkind response, but one of potential shock and dismay to the
As I look through and monitor questions, it is apparent that our field is
declining. These are very basic questions not about special stains or IHC
stains but basic histology that should have been taught in histology 101. My
fear is that as we get older and leave the field, who and what will be
My 2 cents is that she needed to convince someone this was how it is done! J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From:
Assuming you are talking about the aortic valve
Remove the heart and dissect away as much surrounding fat as you can. Leave
some aorta attached.
Make sure the heart is very well fixed in formalin. If the heart is soft and
floppy rather than firm the next step wont go well.
The next
Felton,
I would have to disagree with your assessment of the emails. Our field is
very strong and is not in decline. Unfortunately, some supervisors around
the country are relying on archaic methods and do not want to see or welcome
change within the histo lab. We call ourselves professionals
They do say that in the next 10 years 70% or so of us will be ret
ired. I am 30, and a rarity it seems. I think the main issue wi= th
people not picking up histology is because it is a fairly unknown
field.=I also think that with ASCP slowly moving towards having to
I echo Joyce's point. W/o all info, I hesitate to jump to conclusions. I was
had an efficiency expert following me around for a week at the insistance of
some administrators (it wasn't just me, but the entire lab)These are the types
of silly questions these experts might ask, wondering if they
Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.commailto:jander...@halozyme.com
The information transmitted in this email is confidential and is intended only
Histonet@lists.utsouthwestern.edu
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Hi,
Autofluorescence in atheroma is a well known problem for immunofluorescence
staining.
I would love to hear input about how to reduce it to a minimum (if possible!).
So far, we are trying to detect apoptotic cells in atheroma by TUNEL staining
(Roche, TMRred) and we found the
Calling all histotechs who need a few extra hours!!!
A very good friend of mine is in need of a temp or traveling histotech to
cover the histology duties for 2 weeks... potentially one month. STARTING NOW!
Low volume (40-60 blocks) For more details call Shawn 707-463-7301 Clinical Lab
I will be out of the office starting 09/17/2010 and will not return until
09/28/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
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Hello Histonetters,
We are in the process of selling some of our Ventana Ultraview Detection
kits. The kits still have a long shelf life and have not been registered.
If you are interested in purchasing one of more of these kits please email
me with questions and or contact info.
Thanks,
Hi James,
I would take it a step farther with the continuing ed. I think it's beyond the
supervisors it gets up into lab administration (clinical lab world). I
personally know of a group of great folks that work hard and run a quality
service. In the last 3 years they've had a major drop
I will be out the office the week of September 20-24.
I will return on Monday, September 27th.
If you need laboratory assistance please call 785-273-2788 ext. 322.
Thanks.
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Hi all,
Would you please let me know off line what do you think about this unit?
macve...@usc.edu
Thank you very much in advance
Michelle Aloni MS HTL ASCP
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Good Afternoon!
Please contact me directly for more information on this position or to learn
more about our other opportunities.
Have a great weekend!
Histology Training Specialist
The Company:
Our client is a leading developer and producer of innovative high-tech
precision optics systems
Looking for a CRO experienced in determining the volume of an irregularly
shaped
object from serial sections.
Automation experience a plus.
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