[Histonet] RE: Recommendations for decal solution

5% Formic Acid Thanks Lee Ann -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, October 22, 2010 4:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommendations

[Histonet] Great position for an HT in Nashville, TN

Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse

RE: [Histonet] Wear gloves when handling / cutting paraffin blocks.

We wear gloves we have to sterile section blocks for DNA/RNA extraction. Keeps the slides free from contamination if we are placing sections on slides, otherwise we are sticking them in a vial. All equipment associated with the sterile sectioning is cleaned between each block (block holder, blade

[Histonet] paraffin block mailers

Hi everyone, Does anyone know of a source for packaging exclusive to mailing paraffin blocks? Vendors please feel free to reply as well. Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32

[Histonet] RE: paraffin block mailers

Hi Jeanine, At the NSH vendors I got a sample of a neat clear plastic holder from Source Medical Products, www.sourcemp.com, 1866-735-9965, holds 4 maybe 8 blocks for mailing, depending upon the thickness. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Microwave protocols

Hi Histonetters! I am back with some questions on protocols for microwave processing. We have the Milestone processor. For any of you out there who use the Milestone, do you find the pre-programmed processing runs reasonable for your use or have you modified them? If modified, would you be

[Histonet] Temporary Scientific Associate, Histology, spring 2011

GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures

[Histonet] FW: Saponin Technique

From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the

[Histonet] RE: Saponin Technique

It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of

[Histonet] RE: Saponin Technique

I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740)

[Histonet] Saponin

Lots of blood can be a problem in cyto specimens, especially smears. If you are making smears from fresh specimens in particular you may elute the obscuring hemoglobin by dipping the smear directly in acid alcohol (i think 5% hcl-95% etoh). We do this frequently for CT guided FNA's (particularly

[Histonet] A Good Human Brain Control

Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks ___

Fwd: [Histonet] FW: Saponin Technique

-- Forwarded message -- From: Brandi Higgins brandihigg...@gmail.com Date: Mon, Oct 25, 2010 at 12:08 PM Subject: Re: [Histonet] FW: Saponin Technique To: Hannen, Valerie valerie.han...@parrishmed.com We use Carnoy's to lyse the red blood cells. We get thyroid and lymph node

[Histonet] RE: Saponin

Why use Saponin when you can get a commercially prepared solution such as Lysing 1000, which was specifically designed to remove rbc's from Cytology specimens. Lysing 1000 not only lyses the rbc's but gives you exceptionally preserved cellular material which is amiable with IHC staining.

RE: [Histonet] A Good Human Brain Control

American Matertech Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] A Good Human Brain Control From: Candice Smoots

[Histonet] Thanks everyone!

Thank you to everyone who provided input on preferences for HE stainers last week. You were all very helpful! Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list

[Histonet] Director of Clinical Lab needed can you help?

Hi Histonetters! I hope everyone is having a great day. I just got a new position that I want to tell you about . The position is Clinical Lab Director. The position is with a hospital system and you would have the lab managers from the 5 affiliated labs reporting to you. The position is in

[Histonet] Pouring Chemicals in Stainer

I was curious about any tricks out there for avoiding spills when setting up the stainer. We will be receiving a new stainer soon which will be taller and larger than our current one. I'm just worried I will spill chemical into the surrounding dishes. Would using wash bottles work or are there

[Histonet] Xylene Substitute

Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone use a xylene substitute that you would recommend for this combination? Thank you!  

RE: [Histonet] Xylene Substitute

Sheila, You might want to talk to the Leica rep. My guy was here a couple of weeks ago and they have a xylene sub and a mouting media that plays nicely with it. I don't recall the name of the product, but it might be worth talking to him about it. Good Luck! Michelle -Original

[Histonet] Rubber cutting mats with adhesive backs

Why did they quit making them and what are others using as a replacement? Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 ___ Histonet

RE: [Histonet] Xylene Substitute

We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in our VIP processor and for staining. I am not familiar with Permount, but we use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific) that is toluene based - works great in combination with

RE: [Histonet] RE: Saponin Technique

The following has been found useful in my hands: Removal of Excess Blood Haemolysing haemorrhagic specimens cause fewer problems in identifying individual cells. The removal of red blood cells can be achieved using Ficoll-Hypaque gradient separation or using a lysis solution such as isotonic