Thanks to everyone who replied. No firm answer.
I've told my pathologist that I don't know and may now suggest to one of
the trainees that they ask him.
Finlay
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I thought this would be more interesting to ask people who are working in labs:
The item to the left of you is now your weapon in the impending zombie
apocalypse. What is it?
Sorry about the OT, but I did label it. And hey, we call all learn
how histology helps kill zombies. Or how your mug of
Hi everyone,
Has anyone had a problem with cassettes opening during processing?
Sometimes the cassettes are slightly opened at the top or bottom. We
rarely lose a specimen and it's frustrating to cause the patient to be
biopsied again with no explanation what happened to it. We use Surgipath
Are you sure the cassette is opening during processing? I have seen
many time when small specimens are loose in the cassette the specimen
flips up when the lid is opened and it then falls somewhere else. I
have had this happen to me and my techs more than once.
Mike
-Original
Good Morning Histonet!
We use xylene substitute for almost everything, but I still find that we
need to keep some xylene on hand for certain tasks, especially removal of
coverslips. We use Formula 83 for everything else, but I have let slides
soak in Formula 83 in the past for a multiple days
Hi Histonet Members,
I hope you are doing well. I am a recruiter at a highly successful and well
respected Healthcare recruiting firm. I help place Lab Professionals in
permanent positions across the country and I wanted to see if you are
interested in exploring other career
Gremlins. That's what it is. I recently cut in a case with two parts -
one Extremely Thin Black Crescent of tissue in one cassette and the
other tissue in the next cassette. The next morning, the Extremely Thin
Black Crescent was NOT in the cassette. Gulp. Sometime later, as I was
almost done
My responses to some questions I have received (also FYI for anyone else
with the same issue):
Are you using the tape coverslips or the glass ones? If using the
tape,
it will only be removed with acetone. See what your mounting medium is
based with (Xylene? Toluene? etc.,) and see if
We use Formula 83 and we still have to use Xyene to remove coverslips from
slides that are more than 2 days old.
Joyce Cline
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org
From: histonet-boun...@lists.utsouthwestern.edu
Does anyone use a 9 hour tissue processing that is actually 9 hours. Ours is 10
with the pumpin and out.Thanks!
Jackie Fleming HT ASCP
Technical Consultant - Histology
Phone: 612-863- 4773
Pager: 612-654-2135
e-mail: jackie.flem...@allina.com
This message contains information that is
glass coverslips
On Thu, Nov 11, 2010 at 12:50 PM, Ranostaj, Drew
ranos...@medite-group.comwrote:
Are you coverslips tape or glass that you are trying to remove?
Drew Ranostaj
Medite Inc.
1226 Winter Garden Vineland Rd, Suite 104
Winter Garden, Florida 34787, U.S.A.
Phone: +1 (407) 996
Hi all of you in Histoland!
I want to recognize all of you who have served in the Armed Forces.
I am an old Marine Corp Brat, so Semper Fi and Thank-you! Thank-you!
Thank-you! You gave us years of your lives and took risks on behalf
of your country. I admire you.
Now to the question
Akemi,
Be careful what vendors tell you about being green. As previously
discussed in this forum, Ventana's solution to the disposal of DAB from
their immunostainers is to pay to have the wast hauled off.
Jay A. Lundgren M.S., HTL (ASCP)
Hi,
I'd like to talk with Ellen. If someone knows where to contact her, please
tell her that Diane Miller would like to talk with her. My email address is
dgmil...@coho.net and my phone number is 503-784-6444.
Thanks for your help.
Best Regards,
Diane
Can anyone give me some tips on sectioning mouse spinal cord on the vibratome?
Specifically, once the dura is successfully removed, I have been sectioning 40
um horizontal sections, and some cords have been curling up on themselves, both
rostral-caudal and medial-lateral, and are impossible to
Like everyone we have had a rare occasion when tissue processed did not come
out well at all. My experience over the years has led me to believe that when
there is an entire machine full of poorly processed tissue that the problem is
usually human error where probably one of the chemicals was
Ok, so how many of you are allowed to dump formalin down the drain?
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The labs I worked for in Missouri all dumped down the drain. Now I'm in
California and can't even put ETOH in the pipes.
I'm hoping they have changed practices in MO in the last few years, but doubt
it.
Lori
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I'm in Pittsburgh and we aren't allowed to put formalin or ETOH down the drain.
Deanna
From: Garcia, Lori, Sr. Scientist lori.gar...@medtronic.com
To: godsgal...@aol.com godsgal...@aol.com;
Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
You have to get permission to dump it from you local water people.it varies
by location/state laws etc.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Hi Christine
You have had some good suggestions to date.
Also to consider if you are using the Peloris racks with the individual spaces
for cassettes I have seen this happen when cassettes are inserted into the
racks. As most people load cassettes with the patient details facing up, if
Absolutely not here in southern California.
Sent via BlackBerry by ATT
-Original Message-
From: godsgal...@aol.com godsgal...@aol.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 11 Nov 2010 17:44:52
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin
Not in Florida either. We use Aldex to neutralize the formalin then dispose
of it on the biohazard trash. The mfr says once neutralized you can put it in
regular trash, but we don't.
Michelle
On Nov 11, 2010, at 7:45 PM, jmacdon...@mtsac.edu wrote:
Absolutely not here in southern
I'm looking to find some control tissue that will stain positive for ck7 and
ema. We are currently using breast carcinoma tissue but most of the tumors we
are seeing upon grossing are being submitted entirely for pathologist review. I
have been searching the literature for any normal tissue
John:
Some labs uses processing metod with isopropanol and
mineral oil with very good results.
Here is our manual protocol for rodent tissues:
1. Isopropanol 70% 0.5 h
2. Isopropanol 80% 0.5 h
3. Isopropanol 95% 0.5 h
4. Isopropanol 99% 0.5 h
5. IM 5:1 50oC 1 h
6. IM 2:1 50oC 1 h
7. Mineral oil
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