[Histonet] IHC antibody protocols

Hello All, I need help with information on a few Ventana BenchMark Ultra or XT protocols. We are having trouble getting HCL (DBA.44) to work. We are also in the process of working up FOXP-1, TAI-1, and GCET-1. Your help is appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology

Re: [Histonet] Frozen brain slices

Thanks Tina. I will keep in mind the thickness for staining purposes that is once the slicing protocol gets optimized. So we use 200-300 ml of both PBS and PFA for our perfusion. So the post fix and sucrose treatment you are suggesting, are you suggesting for the new harvests alone which I will be

RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner)

We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Annual Review of Manuals

For those of you who work in/manage CAP accredited laboratories, what is your current understanding/policy in regards to annual review of all relevant procedure manuals by all personnel? If you no longer do this, what procedure manual review IS done in your lab? Thanks, Lester J. Raff, MD

[Histonet] prostate biopsies

Can anyone share their processing schedules for prostate biopsies using a variety of processors? Examples: Sakura VIP, Tissue Tek express, Leica Peloris, or Thermo Pathcentre? What is the optimum rapid processing schedule? Do you prefer to use a certain kind of alcohol?

[Histonet] Procedure manual review

All procedures must be reviewed annually (and that means WITHIN 12 months, not just once a calender year) This may be done by the appropriate supervisor or pathologist, not necessarily the Director (Director must review all new or changed procedures) The review should confirm that the procedure in

RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner)

I'm looking at the Slide Mate and Print Mate, too. Currently, I have the Leica cassette and slide printers. They have really been great. Reliable and relatively problem free. But they are so large. I'd like to find a slide printer that has a small footprint, like the Slide Mate, so that we can

[Histonet] Tap Water or filtration system

We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to

[Histonet] glutaraldehyde fixed tissues and genomic DNA

Hello, I have PFA+glutaraldehyde fixed tissues that I might want to digest down in order to extract genomic DNA suitable for bisulfite sequencing. The tissues are embryonic mouse heads. The fixative is a PFA + glutaraldehyde mix: 3.6 PFA with 5% glut. The glut is grade 1 or electron miccroscopy

RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner)

I agree with Laurie. We have seen a great improvement in efficiency and reduction of errors with this hardware. We have been using both for a few months now and have not had many problems. Jaime McMillan -Original Message- From: Laurie Colbert

[Histonet] Non-Refrigerated RPMI/3-in-1 Pap Stain

I would like to obtain information to order the non-refrigerated RPMI. And does a 3-in-one Pap stain exist? Someone in a workshop in Seattle said they knew about it but couldn't give the exact supplier. Thank you for your help. M. Fisher El Centro Regional Medical Center www.ecrmc.org

RE: [Histonet] Tap Water or filtration system

I don't have the Lecia Autostainer but have a Surgipath Tribune stainer. I have it connected to tap water and do both HE and PAS on it. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa

RE: [Histonet] Tap Water or filtration system

We use tap water on our Leica. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, November 15, 2010 13:15 To: 'Vanessa Avalos'; histonet@lists.utsouthwestern.edu Subject: RE:

[Histonet] Slide Mates and Cassette printers

We have three of the older six hopper Thermo Micro cassette writers and one Thermo slide mate, in addition to four of the Pslims. The Pslims are the first generation Slide Mate by Accuplace. They are all connected to our APLIS System (PowerPath) and we use 2D bar-codes on our specimen labels,

Re: [Histonet] Tap Water or filtration system

We always used tap water, without any problems. Vanessa Avalos vava...@allergydermatology.com Sent by: histonet-boun...@lists.utsouthwestern.edu 11/15/2010 09:54 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tap Water or filtration system We are in the process of

[Histonet] pathologists

Would any of the pathologists or histotechs know what the average volume of work for a urology pathologist would be for a given day? How many slides/cases is a comfortable workload for one day of reading, say in regard to prostate biopsies or bladder biopsy cases (excluding vas deferens)? Also

[Histonet] IHC Mohs

Hi Everyone!! I need advise on getting started with IHC on Mohs sections! Vendors, automated vs by hand, antibodiesanything and everything will be s greatly appreciated. Please and Thank You in advance!! ___ Histonet mailing list

[Histonet] RE:Tap Water or filtration system

What if we suggest to use ddH2O both for TBST preparation and in autostainers (before and after IHC) because the PH level is vary in different states. Naira ** Confidentiality Note: This message (including any attachments) is intended for the use of the named recipient(s) only

RE: [Histonet] pathologists

It is important to understand details of slide preparation. We prepare six levels of each block/ each glass slide. A case is prostate biopsies from one patient. 85% of the time we get two biopsy cores per container and thus two cores per block, and 12 cores total per case. A busy day is 12

[Histonet] Control sections for frozen sections

Does anyone run a HE control prior to staining a frozen section? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Tap Water or filtration system

We use tap water and have had no problems. Sydney tap water seems to be quite innocuous Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and

RE: [Histonet] Tap Water or filtration system

We use tap water in our Leica multistainer for HE's and a few other stains. The protocols can be optimized to accommodate what ever you are using. I can't speak to the PAS stain except to say that even if you need deionized water, you can buy it in 5 gallon cubes for limited use and still save

Re: [Histonet] Cardboard slide flat storage

Hi Meghan, We use grey colored boxes that will hold either 8 cardboard drawers of blocks or 4 cardboard drawers of slides. I am at home right now, don't have the specific ordering information, but will share it tomorrow when I get to work. I just didn't want to let the question languish w/o