RE: [Histonet] Refurbished histology Leica equipment

We have the Leica XL autostainer and CV 5030 coverslipper, both refurbished units, and are very happy with them. We saved a lot of money by doing so. We purchased both from Medical Equipment Source. We also purchased a Leica RM2255 automatic microtome as well. Peggy -Original

RE: [Histonet] Tap Water or filtration system

We also use tap water, but have a filter unit attached. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu

[Histonet] Control sections for frozen sections

Hi Diana, We run one frozen section HE every morning after the frozen section staining reagents have been filtered, changed or topped up. The QC slide is checked by the technologist and the results recorded and initialled on the QC sheet. Lee Hamilton, Ontario

[Histonet] neurodegeneration stains?

Hi all, I am trying to characterize neuronal degeneration and was wondering if there are any fluorescent markers out there to stain for dead/dying neurons? I have used Silver stain and am currently trying to optimize the Fluro Jade C marker too. But I need some more and the literature seems

RE: [Histonet] Cardboard slide flat storage

Meghan, This is the catalog number for the slide/block storage boxes we use: 22-272-957 and we get them from Fisher Scientific. The following is the link on fisher's website: http://www.fishersci.com/wps/portal/PRODUCTDETAIL?prodcutdetail='prod'produ

[Histonet] Partially muddy slides

Hi Histonetters! We are having an issue in our lab with some inconsistent staining. It's an odd situation. One small biopsy can stain reasonably well over most of the tissue, but then one area of it is muddy. This does not affect all the slides in the same staining rack, nor does it affect all

[Histonet] Disposable blade holder for a Leica 1512 rotary microtome.

I hope that someone on histonet could help me. Our research lab just acquired an old Leica 1512 in good working order but lacking a blade holder. Is it possible for us to find a blade holder for this machine or is this a futile cause? Any information you all could provide would be greatly

RE: [Histonet] neurodegeneration stains?

Anjum Those are the two that I'm familiar with. I'm not aware of any others. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive,

RE: [Histonet] Partially muddy slides

Regarding incomplete deparaffinizing: I did give that some thought, but wouldn't that affect ALL the slides and not just hit or miss? It's primarily the small biopsies that are being affected, and I would think those would clear the paraffin much easier than the big specimens? Just as a

[Histonet] connexin 43 ab

Dear Histonetters Does anybody have a preference for a connexin-43 ab? A registrar needs it for a research project. I am leaning towards the Sigma C6219 rabbit plyclonal. I would appreciate any suggestions. The material would be ffpe human heart tissue Many thanks Heather McLeod

[Histonet] Vol 84, Issue 20 Non-Refrigerated RPMI/3-in-1 Pap Stain

For those who were in Seattle this is the info you are looking for ... The RPMI storage temp is +15 C to + 30 C and we purchase ours from Fisher (cat# 12-167F Lonza BioWhittaker RPMI-1640 500ml www.fishersci.comhttp://www.fishersci.com/ The 3-in-1 Pap is called EasyPap stain and we

[Histonet] FW: What % of IHC Stains Should You Expect to Repeat?

WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended

Re: [Histonet] Partially muddy slides

Is it the same person cutting all of the problem biopsies? Does your lab use cryo spray? We had biopsies that had a muddy/freezer burnt appearance. We traced all of the problems to one histotech who used the cryo spray excessively. histot...@imagesbyhopper.com Sent by:

[Histonet] Biocare¹s Rodent Decloaking Solution

Hi everyone, For anyone that uses Biocare's Rodent Decloaking Solution, once the solution has been made up, can it be reused? And if so in your experience how many times? Many thanks in advance ___ Histonet mailing list

RE: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat?

Send the colon cancer control out and compare your results to the send out. If they are the same then you have an argument. From: wanda.sm...@hcahealthcare.com To: histonet@lists.utsouthwestern.edu Date: Tue, 16 Nov 2010 13:07:28 -0600 Subject: [Histonet] FW: What % of IHC Stains Should

RE: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome.

Klaus Dern at 706-635-8840 will make you one that works like a regular knife holding a disposable blade. I copied everyone in case someone else needs the info. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone

Re: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat?

That type of pathologist deserves an answer that could cost you your job, so it is not advisable to do that. That type of pathologist is essentially ignorant of what we do and do not reason. If he wants to send everything out, let him. When charges begin to hit your administration, perhaps he

RE: [Histonet] Partially muddy slides

Michelle, It might possible be water contamination in the xylene. Try increasing alcohol dehydration times. Also re-cyled alcohol is probably around 96% pure. It seems to be not possible to obtain absolute ethanol by re-cycling, or so I am lead to believe. Regards Tony Henwood JP, MSc, BAppSc,

RE: [Histonet] Frozen brain slices

Thanks Tina. I will try that for the already frozen brains. Also I did some slicing today with some of my frozen brains and kept the block in the cryostat till it came to temperature and as compared to before I think I can say that the slices came out pretty well. I guess the temperature is the

Re: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome.

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[Histonet] Current issues with Printmates and Checkmates

Hello? Is anyone having issues with the Checkmate and/or Printmates? What is the opinion out there about using the hot foil tape methodology? Is the tape chemically resistant? Histopathy ___ Histonet