[Histonet] Histotechnologist position in DC

2011-01-14 Thread Nichole Ramis
Slone Partners seeks a Histotechnologist for a cutting edge hospital laboratory, based in a beautiful Washington DC neighborhood. ASCP certification is preferred with at least 2 years of experience in a high-volume laboratory. Special features of the position: This organization embraces

[Histonet] Request for Coverslip Removal SOP

2011-01-14 Thread Sean McBride
Hi colleagues, I need to remove a glass coverslip from a mounted HE biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks

[Histonet] Antigen Retrieval for 10u sections in IHC

2011-01-14 Thread Margaryan, Naira
Hi tistonetters, I have to do IHC on 10µ sections. Is procedure for Antigen Retrieval same like for 4-5µ (time and temperature) Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Request for Coverslip Removal SOP

2011-01-14 Thread Patrick Laurie
soaking in xylene for at least a couple of hours (overnight is best) works for us. On Fri, Jan 14, 2011 at 11:55 AM, Sean McBride smcbr...@andrew.cmu.eduwrote: Hi colleagues, I need to remove a glass coverslip from a mounted HE biopsy slide with an implant in order to run some biomaterial

RE: [Histonet] Request for Coverslip Removal SOP

2011-01-14 Thread Setlak, Lisa
Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM

[Histonet] decalcifying bone marrows after processing

2011-01-14 Thread Powers, Kerry
I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper

Re: [Histonet] decalcifying bone marrows after processing

2011-01-14 Thread Rene J Buesa
If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? It is preferable to use formic acid (even if it is worse than using EDTA) than having to struggle with a poor section produced and then trying to decalcify it. This is typical of the