Re: [Histonet] Where can Fast Blue (Diamidino compound 253/50) be obtained?

2011-02-05 Thread koellingr
Hello Per, Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.  Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good

Re: [Histonet] Amylase Digestion for glycogen

2011-02-05 Thread Rene J Buesa
You are dealing with synonyms of the same enzyme but the amylase protocol always is slightly more complex. Stick with diastase and, please, never use your own saliva for this procedure René J. --- On Fri, 2/4/11, Ruppert, Amysue ruppert.amy...@marshfieldclinic.org wrote: From: Ruppert,

RE: SPAM-LOW: [Histonet] CE

2011-02-05 Thread Patsy Ruegg
It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original

RE: SPAM-LOW: [Histonet] CE

2011-02-05 Thread Patsy Ruegg
Also I just thought of the exercises in JOH, it think they are giving some CEU credits for them, plus the traveling seminars given by NSH the rest of the year apart for the NSH S/C, see if any are in your area. The IHC Forum is going to be in the Denver area in July this year, July 16 I believe,

[Histonet] RE:Acetylcholinesterase

2011-02-05 Thread Hobbs, Carl
Hey! The GREAT Brian Lake was THE Man behind it all, imho. Respect to Him. Filipe, jest like Hiederman/Heyderman?, became famous because of the people/technicians who did all of the work and...never got the credit ;-) Carl Hobbs Histology Manager Wolfson CARD Kings College London 020

[Histonet] TTF-1 Staining

2011-02-05 Thread Robert L. Lott
Subject: TTF-1 Staining For those of you who participate in the CAP/NSH HistoQIP program... and did so in back in 2009, one of the IHC challenges that year was TTF-1. From the critique published with that particular challenge, enrolled participants may have read with interest the following 2

[Histonet] Teleconference CE

2011-02-05 Thread Lee Peggy Wenk
(Disclaimer to start - I'm the NSH Teleconference coordinator. But I don't get paid for this position. I volunteer to do this, as I believe in continuing education for all histotechs.) Thought I'd expand a little about the NSH teleconfereces. 4th Wed. of each month (Nov and Dec. might be 3rd

[Histonet] Looking for position in Texas

2011-02-05 Thread Wilson A
    Hello all, I am looking for a new histotech position in texas, especially in the houston area so as to be,  very close to my family. Though, any part of texas is okay.     I am an HT(ASCP) certified histotech. I do have substancial years of experience and with QIHC(ASCP)    

[Histonet] Re: Amylase Digestion for glycogen

2011-02-05 Thread Nathan Jentsch
We use amylase digestion for our daily PAS/D stains. The procedure is not that complicated. We preheat the enzyme in a 45 degree C water bath for 10 minutes. Then we let the slides digest with the solution still in the water bath for 10 minutes. Then we rinse in running tap water for 5 minutes