Dear histonetters,
I have pasted two images img001 and img002 dt. 7feb2011 on staining. The images
are of mouse kidney 4micron paraffin embedded formalin fixed sections where
the hematoxylin stained areas are pale and not uniform. Is it because of over
heating or sections getting dried during
Amysue,
Our amylase digestion procedure is quite simple (see: Mangan, V-M, Farago, V.,
Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for
the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153.) See
below for the method:
Amylase Reagent
Warning: Harmf
