[Histonet] Re: Picro sirius red, fast green connective tissue stain

Hi, John The method is a two color staining method that stains collagen in red and muscle fibers in green. The filtration is in order to make the solution that is being used multiple times and stored for a long period, more clear. I've attached an article with an example (Fig2), that is done in

[Histonet] microtome safety

Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special

Re: [Histonet] microtome safety

Every tech in my facility uses something different. Some use fingers, some use forceps (usually curved), one uses a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage using one's fingers too close to the blade. Hope this helps.   Sheila

[Histonet] Measure productivity by skill level

Dear Fellow Histology Professionals, My senior manager give me a task to present a presentation on how to measure and calculate the work lode for manpower (productivity by skill level), we already use Welcan units. Does anyone know who can I prepare? Your input would be really appreciated,

RE: [Histonet] microtome safety

Same here. One tech keeps her index fingernail extra long for this purpose. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila Haas Sent: Tuesday, March 08, 2011 8:26 AM To: Bartlett, Jeanine

RE: [Histonet] microtome safety

I've always used my fingers but some others in my lab use forceps. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] microtome safety

I always found better and used a wet camel's hair pencil. It provides the most gentle pull on the sections. René J.     --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Subject: [Histonet] microtome safety To:

[Histonet] RE: microtome safety

Thanks everyone, I think I have enough information. I really appreciate all the replies! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov _ From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday,

[Histonet] RE: microtome safety

My mentor used forceps. Thus I have always used forceps. I think the paraffin ribbon would melt onto fingers on a warm day. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From:

RE: [Histonet] microtome safety

Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From:

RE: [Histonet] microtome safety

Actually I need to qualify my answer, I use my fingers to grab end of the ribbon the farest from the blade and curved forceps near the blade so I use both finger and forceps at the same time. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax

RE: [Histonet] microtome safety

I use forceps, the thinner the tips, the better. I've had people in here who use brushes, too. I can't use my fingers, the ribbons always stick. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State

RE: [Histonet] microtome safety

I use the end (without bristles) of a paintbrush Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Re: band saws

Hazel Horn in Little Rock AR asks: I am looking for a band saw to cut our bone tumors. What do I need to be looking for? Power? Size? Other suggestions? I don't have any personal experience with it, but at a program about orthopedic pathology about 10 years ago I heard about a hardware-store item

[Histonet] RE: microtome safety

Both. Fingers to lift one end of the ribbon as I cut it and then with the other hand I use a small brush to lift the other end from the microtome. Allows me to stretch and smooth the ribbon as I lay it out on the water bath. Some others here us forceps, picks, etc. Tom McNemar, HT(ASCP)

[Histonet] HT Position New Mexico

know what else I can assist you with . Thanks Great opportunity for a Histotechnician in a brand new laboratory! Digestive Disease Consultants of Las Cruces , NM is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA

[Histonet] RE: microtome safety

I do the same as Tom. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original

[Histonet] cell blocks

So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the boogers dried up and

[Histonet] Controls for IPs

Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for

RE: [Histonet] microtome safety

Just regular forceps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

RE: [Histonet] Alcohol Xylene recyclers

We use Creative Waste Solutions recyclers. We have alcohol and formalin recyclers and utilize xylene absorption pads to get he water out of the xylene and reuse it. We choose these recyclers because they are a gravity feed system that does not require a power source, they are quiet so we can

RE: [Histonet] Alcohol Xylene recyclers

The CWS recyclers didn't work for us because of volume and too many bloody specimens.We have to use the B/R recyclers. Jackie Fleming HT ASCP Technical Consultant - Histology Phone: 612-863- 4773 Pager: 612-654-2135 e-mail: jackie.flem...@allina.com From: Feher, Stephen Sent: Tue

re: [Histonet] ArrayMold System

Hi, I am also interested in hearing about the ArrayMold vs Beecher vs any other do-it-yourself TMA systems. Any favorites systems/recommendations or suggestions? Thanks ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute re: Wondering if anyone has used/purchased

[Histonet] Laura Miller is Out of the Office.

I will be out of the office starting 03/06/2011 and will not return until 03/14/2011. I will be out of the country until Monday, March 14th. While I will be checking messages, my response time will be delayed. Thank you __

[Histonet] RE: cell blocks

Sarah, The clear rite is the problem. We have it on our processor for routine paraffin processing and it works well. But if we use histogel or agarose, we make sure to use pure xylene ( not even recycled xylene). In past experiments, we have found that in the clear rite station the gel

[Histonet] Herpes Virus

All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Preferred green counterstain for GMS?

Hi, What is your preferred green counterstain for GMS? In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine. Which should I buy? Any help is appreciated! Thanks! ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information

[Histonet] RE: Herpes Virus

We use Cell Marque RTU 361A-18 362A-18 And mix them together to make our own. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From:

RE: [Histonet] Herpes Virus

I am really interested in this also. And in addition to the Simplex I and II, does anyone know of a good antibody for Zoster (Varicella)? Thanks, Sheila Fonner HT (ASCP) KDL IHC Knoxville, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] microtome safety

I use curved forceps to hold the ribbon and a paint brush to release it from the knife edge. The curved forcepts help to separate the sections and remove air bubbles. Jennifer MacDonald Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent by: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Herpes Virus

We get the single Abs from Cell Marque, polyclonals, and cocktail them ourselves. I learned the hard way that vendors are no longer able to sell these already cocktailed. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI

[Histonet] Testing for Chemo Sensitivity

Hello all! I was wondering if anyone has recently sent out a specimen for chemo sensitivity. If so where? Does the specimen need to be fresh/fixed? Is there a kit? Thanks in advance for your responses!!! PETE ___ Histonet mailing list

[Histonet] RE: Histonet Digest, Vol 88, Issue 10 microtome safety

I use anything except for a wooden pick. The pick is hard to use. I prefer the curved forceps and a brush, but depending on what is readily available a teasing needle or my'fingernail'will have to do. I try not use my nail, due to the safety risk, but in a pinch it works. Toysha N. Mayer,

Re: [Histonet] Preferred green counterstain for GMS?

Our lab uses Light Green SF yellowish. Both Carson and Shehan's procedure (adapted from AFIP) use Light Green SF yellowish. However either Light Green SF yellowish or Fast Green FCF imparts a green color to all components of the section (J.A. Kiernan). Tartrazine is yellow. Good luck! On Tue,

[Histonet] Re: Bone marrow smear fixation

We are air-drying our smears. I have worked other places that fixed them for 5 min. in Methanol. Maybe do both and compare the morphology. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton Hamilton, NJ 08610 609-631-6996

[Histonet] Measure productivity by skill level

I would also be interested to know that. I am not even familiar with Welcan units. Would someone please elaborate? Michelle Aloni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] AUTO: is out of the office. (returning Thu 03/17/2011)

I am out of the office from Tue 03/08/2011 until Thu 03/17/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mi...@leica-microsystems.com, for product management support or Karen Niewerth,

[Histonet] RE: Preferred green counterstain for GMS?

My mentors way back taught us to use Methyl Green as a counterstain for GMS because it also stains nuclei, but it has to be cleared with chloroform to remove Methyl Violet contamination. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James

[Histonet] Computerized inventory system

Hi everyone, Just wondering if anyone is using or heard of any lab inventory system on the market. We have been looking at a system called Cove.Can anyone give me any feedback Thanks, Andrea Beharry Technical Specialist- Pathology William Osler Health System

[Histonet] RE: cell blocks

Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845

[Histonet] RE: Preferred green counterstain for GMS?

Ally, I would use Fast Green FCF. It does not fade as much as Light Green. Use a 1% in 2% Acetic acid. Tartrazine will give you a yellow colour. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845

Re: [Histonet] microtome safety

We use fingers...I am not aware of any kind of forceps. But along with you i am also curious to know if any thing else is available. Amita From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 08/03/11 06:52 PM