[Histonet] Storing slides in buffer

Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to

[Histonet] staining of damaged mitochondria

Hi, Do you know a way to stain damaged mitochondria in rat hearts after ischemia/reperfusion by immunohistochemistry? Thank you very much! Felix -- Felix Nagel Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle

Re: [Histonet] Light hematoxylin staining in HE

Are you soaking your blocks in dilute ammonium hydroxide before sectioning? Soaking too long will affect stain quality. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902)

RE: [Histonet] Bielschowskys Stain

Glawon, Thank you for your help. I appreciate it. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 2:33 PM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Betsy, I don't know of any modifications for Eager's but there

RE: [Histonet] Storing slides in buffer

I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From:

[Histonet] CONTROLS

Hello everyone, I have an issue that I would like some help with. We are currently running SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the regulations being so strict regarding the processing times of breast specimens, how are you all dealing with that when it comes

[Histonet] Procedure for making Gram Control

Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi /pre--- Horizon Health Network Disclaimer ---brbrThis e-mail communication (including any or all attachments) is intendedbronly for the use

[Histonet] RE: Procedure for making Gram Control

Making you own...not sure how to do that, but Slim Jim's work in a pinch. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax)

[Histonet] PRN Position in Longmont Colorado

Hey all, There is a PRN position at Longmont United Hospital open. Working Mon-friday no weekends no evenings. I have attached the link to apply. http://longmontunitedhospital.force.com/Careers/ts2__JobDetails?jobId=a0IA002ct4yMAAtSource= thanks Matt Lunetta HT(ASCP) Longmont United Hospital

[Histonet] Re: Storing slides in buffer

It makes sense that some epitopes might be more sensitive than others. For 48 hours, I would probably store in just PBS, and leave out the detergent (I am assuming the 'T' in your PBST is Tween). Even though Tween is a weak detergent, I would worry that long-term detergent treatment might dissolve

[Histonet] Making gram controls

Randi,   I don't have a written procedure to forward to you, but this is what we do when we make our gram controls. We use fresh tissue from an autopsy before it goes in formalin (such as lung, we've used placenta before as well). Then put a couple pieces of the fresh lung into containers

[Histonet] S100 A1

I'm looking for a mouse anti human S100 A1 antibody for IHC. My vendor doesn't carry it anymore. Anyone have one that they are using on FFPE tissue? Could you send me ordering info or a copy of your data sheet? Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology

[Histonet] Light hematoxylin staining in H%26EIn-Reply-To=

We seem to be constantly battling this as well. We have found that the quality of the ethanol used in processing and staining can be an issue. We now purchase ours from a company at www.UltraPure.com. The quality of the DI or distilled water being used can also be an issue. We are currently in

[Histonet] CAP checklist, question ANP.23041.

Regarding CAP checklist, question ANP.23041. The operation of the imaging system is performed by high-complexity testing personnel. We have a question regarding the qualifications of the operators. The operators of the Aperio system are simply scanning entire slides to make a record for

[Histonet] Research and Clinical Labs

For 10 years I ran Histology lab for a large University. We used the same equipment and lab for both animal and human tissue. I had to certify the lab with CLEA for human tissue at one point (and this took serious work!). If you are already certified for human, using the more strict regulations

RE: [Histonet] CAP checklist, question ANP.23041.

Mark this is a new question and let me answer this for you from a inspection point of view. Currently there are 15 to 18 new questions that deal with predicative markers and the digital images, most of these are QA/QC related. But the issue with images is that your people are inspecting them.

Re: [Histonet] CAP checklist, question ANP.23041.

Well I wouldn't try and use a Ph.D. in religous studies to qualify for high complexity testing... On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner mtur...@csilaboratories.comwrote: Regarding CAP checklist, question ANP.23041. The operation of the imaging system is performed by high-complexity