A pyrometer should do the job, I guess.
My last CLIA inspection, I was told to check the temperature in the wax
chamber of my embedding station to compare against the temperature it
says it is...does anyone know of a good thermometer to purchase?
The easiest way to remember is to count the midnights between starting and
ending - therefore today will be 0, tomorrow 1etc
On Mon, Apr 18, 2011 at 6:24 PM, Jenny Vega histotech...@gmail.com wrote:
Yes, that is what makes sense to me. Tomorrow morning after removing the
specimens from the
Hello out there!
I was wondering if any of you have experience in attaching human retinae
(~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost
does not seem adequate.
Any advice would be greatly appreciated!
Regards,
Abbey
Abbey Mortimer
PhD Candidate
School of
There is a full time position open for a Cytotechnologist in Brooklyn, NY. A
candidate with Histology background is preferred. Please forward letter of
interest and resume to
Ellen Barbarino
ebarbar...@siradonc.com
Thanks
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Hello,
I'm investigating a potential neuronal defect of our transgenic mouse
embryos.
Until now the staining I tried (luxol Fast Blue) didn't work out.
I was wondering now if there is an alternative for this.
Is there a staining (or an Ab), that I can use to detect any kind of
Hi Hendrik,
Try rabbit neuro-specific beta-III tubulin antibody (Abcam ab18207). Works fine
on frozen and paraffin sections.
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail:
Following is question on behalf of Jana Moose Ritter, DVM
janamo...@yahoo.com
We are going to perform IHC on frozen sections, and the tissues (pig
aorta) will be harvested on a Friday.
Histo lab is wondering:
1) can tissue fix in paraformaldehyde over the weekend until next
Good afternoon:
I am Carmen Garcia a PhD student from the Faculty of medicine from the
University of Valencia.We work with cryocut of mouse ovary, now I want
to see the filopodia of the endothelial cells, do you have any
suggestion about what I should do?
Thank you so much,
Carmen
** Proprietary **
** Reply Requested When Convenient **
Hi there,
Does anyone use commercial Miller's Elastic Stain other than VWR Ref 35115S one
?
If so I would be grateful if you could let me know which one you use
Thanks in advance
Malika
Malika Benatti
Specialist BMS
Camelia Botnar
SAME HERE!!! I would love some help with this. We are going to start doing
the EBER with Ventana and have no idea where to start.
Thanks in advance for ANY suggestions or help you could give.
Sheila, HT (ASCP)
KDL Pathology
Knoxville, TN
From: ih...@googlegroups.com
If anyone has the catalog numbers for the probes, that would help. I
couldn't get this info from Ventana when I asked. They said we have to ask
another lab, even for catalog numbers!
Mark
On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote:
SAME HERE!!! I would love
Your sales rep should supply these to you.
I'm having problems with filling my ISH Probe Kit. I logged in my EBER Probe. I
registered the Probe kit and went to fill it and it only lets me pick from my
Fillable Reagents list, not the Fillable probes list. Tech support can't figure
out why this
Try to eliminate one of the 100% Alcohols. If it cannot eliminate it, then
just pump it in and out.
Microwave processing actually uses Ethanol, Isopropyl, and paraffin only.
Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD
You can bay a certified digital thermometer for less than $35 and will be more
than adequate for what you need.
René J.
--- On Mon, 4/18/11, Amber McKenzie amber.mcken...@gastrodocs.net wrote:
From: Amber McKenzie amber.mcken...@gastrodocs.net
Subject: [Histonet] thermometer
To:
Such thick sections are usually handled better if stained floating (not adhered
to sledes).
René J.
--- On Tue, 4/19/11, Abbey Mortimer a.morti...@latrobe.edu.au wrote:
From: Abbey Mortimer a.morti...@latrobe.edu.au
Subject: [Histonet] Tissue adhesion to slides
To:
This questions is for the labs that do in-situ hybridization for diagnostic
purposes. Do you take slides for ISH from other labs and if so, please contact
me via my email.
My next question is; one of my doc's is interested in us doing ISH herein our
lab but I have been putting her off with the
I'm looking for recommendations for a new slide drying oven. Something
relatively small, with convection. Any input would be appreciated. Thanks !
Mandy M Bell , HTL (ASCP)
Histology Department
Community Hospital of the Monterey Peninsula
831.625.4791
P Please consider the environment
Hello Everyone,
Need some info on the disposal of gross only items.
These items include hardware, wires, gallstones and foreign objects.
We are running out of room for the storage of gross only specimens.
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There are many so called convection ovens out there that are gravity
convection. Biocare has a great convection oven that has an internal
fan, and can be set with several different programs. You can set it
for IHC at the end of the day and it automatically stops and is ready
for you in
Hi all,
I'm looking for something to hold my rabbit eyes in place (and other eyes if
they're available like rat) while cutting them in half before further
processing. My boss said he used them a long time ago but doesn't know what
they're called. I can't figure out what they'd be called
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In the re-analysis, results of which were just announced, a total of
87 women were found to have received a false negative result from the
original
Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is
faithful to the healing ministry of Jesus Christ, providing high quality care
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The information contained in this communication, including
Roche Diagnostics has great DIG labels, antibodies and color reaction
chemicals.
It is a pain to just start up, you need to buy a lot of stuff for it. Could
you use someone else's set up and pay for part of the chemicals?
Emily
A great book should leave you with many experiences, and slightly
From what I have experienced in my observations of various labs;
numerous labs do not follow proper standardization practices which
are set by FDA guidelines for Her2-neu. Prognostic markers are
particularly susceptible to heat. Baking slides in excess
temperatures (over 62 degrees C) for
Wow, somehow I missed this email. Sorry for the delay.
Andria, take the heat off your alcohols. Keep it on the formalin and the
paraffin, but do the rest of the processing at ambient temperature. Make sure
you put only small soft tissues on this schedule though, tougher collagenous
skin
Does a change in the type of hematoxylin counterstain require any re-validation
of IHC stains?
Thanks for your help!
Mary Ring, HT, QIHC
Regions Hospital, St Paul, Mn
This e-mail and any files transmitted with it are confidential and are intended
solely for the
Are you using tissue microarrays as controls for all your routine IHC stains
(not antibody validation or research) ? If so, how are you making the arrays?
thank you,
-Kiranjit
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Histonet@lists.utsouthwestern.edu
First talk to your legal department, just in case!
René J.
--- On Tue, 4/19/11, Stella Mireles estellamire...@gmail.com wrote:
From: Stella Mireles estellamire...@gmail.com
Subject: [Histonet] Disposing of gross only items
To: Histonet@lists.utsouthwestern.edu
Date: Tuesday, April 19, 2011,
Not really because the Hematoxylin should affect only the nuclei UNLESS it is
a nuclear antibody, in which case the antigenic reaction could be obscured.
For nuclear Abs I used to dilute my hematoxylin as much as possible or did not
use it at all.
René J.
--- On Tue, 4/19/11, Ring, Mary L
In my opinion, only if it affects immunoreactivity.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860)
I am out of the office until 04/21/2011.
I will respond to your message when I return.
Note: This is an automated response to your message Histonet Digest, Vol
89, Issue 21 sent on 4/19/2011 12:02:59 PM.
This is the only notification you will receive while this person is away.
Dear Abbey Histonet
I agree with René Buesa: I would try to stain whole retinae by a 'floating
section' method i.e. hand processing in a dipping tray or mesh baskets (brush
transfer can be done too but is laborious). Then mount them at the end.
Floating allows reagents to penetrate from both
Jana,
Since you are fixing in formaldehyde, why not paraffin embed them?
Frozen sectioning of formalin fixed tissue and doing IPX on them is difficult
as well as problems with storing the tissue.
I would cut frozen of unfixed tissue and do IPX on them rather than doing IPX
on formalin fixed
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