Hi I have just diluted my DPX with xylene and my god its working a treat,
thanks to all who helped me your very kind. fingers crossed it keeps this
up!!
Thanks Sara
_
From: Paula Pierce [mailto:cont...@excaliburpathology.com]
Sent: Tuesday, May 17, 2011 3:37 PM
To: Lees,
Hi All
We are currently trying to stain for calcium deposits using Alizarin Red. A
few months ago our protocol worked fine, but this time we are getting zero
staining. Reagent has been made fresh, and pH is between 4.1 and 4.2.
Here is our protocol.
Deparaffinize to distilled H20
Stain
I have a new carbon filter for a Leica Coverslipper Model CV5000 that I ordered
by mistake. It is from Mercedes Medical, part #MER440122. It is free to whoever
will pay the shipping to your facility.
Thanks,
Robin Taylor HT
Butler Cty Medical Center
3145 Hamilton ason Rd.
Hamilton, OH 45011
We currently have a Ventana Benchmark XT and are looking at the Leica Bond
III. Has anyone made the transition from a Ventana to a Leica recently and
how difficult was it?
Jennifer Thawley HT, ASCP
Histology Supervisor
Shore Memorial Hospital
(609) 653-3577 ext. 2084
This transmittal from
Karie,
Just a little input from a newbie. I am researching stains and fixatives
for causes of error in one of my courses. For the hematoxylin precipitate
attributed to inadequate filtration, I found a site that discussed this,
along with formalin pigment, mainly that sometimes precipitate
For Karie's query;
I have just a little input from a newbie. I am researching stains and
fixatives for causes of error in one of my courses. For the hematoxylin
precipitate attributed to inadequate filtration, I found a site that
discussed this, along with formalin pigment, mainly that
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we get
one
Curt,
Nonsense. The negative control is used to evaluate endogenous staining
in the *patient* tissue. Your Pathologist needs to do another residency at
clown college.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
Hello,
I would like to repeat my DAB staining on the same slide with DAB I run before.
I know that acid alcohol will remove hematoxylin but how to remove DAB?
I appreciate to any suggestion.
Thanks in advance,
Naira
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Dear Amanda, if you have the staining solution well prepared (the colour must
be like dark iodide solutions, I recomend you to check ammonia solutions) the
problem should be that you are loosening your calcium deposits. May be you have
sth wrong or may be some EDTA contamination in the fixative
Can anyone recommend a good commercially available kit for BrdU on rat
tissue?
Thanks in advance for any suggestions.
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Our municipality actually came here and told us not to put anything down the
drains. We now have it all hauled off they told us we can't even put the 10%
when it has been treated down the drain.
Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
I think that depends on the state you're in and what the regs are. I'll
forward this to my vendor who makes our formalin neutralizer, He may be able
to help out. There are several different products I think but not all are
state approved. I had to find the one that is approved here, in cali.
Curt
Hello Histonet community,
We recently obtained a refurbished Tissue Tek VIP 2000 tissue processor and
now wish to set up a few programs for processing. The operating manual lists
a sequence of fluids, times, and V/P cycles etc. for one generic program. I
wish ask if anyone out there might share a
I think this approach mixes up antibody-validation and analysis.
The pathologist should be confident, that the used antibody is validated
with several types of tissue and that the special antibody stains positive
epitopes positive and negative epitopes negative.
But the point is, that the patient
The ideal situation is as follows: a known (+) control with the patient's
tissue to make sure that the reaction worked, and a (-) using a section from
the patient's tissue to rule out any false (+).
René J.
From: Curt Tague c.ta...@pathologyarts.com
To: histonet@lists.utsouthwestern.edu
Sent:
DAB reaction is almost permanent and extremely difficult to destain.
René J.
From: Margaryan, Naira nmargar...@childrensmemorial.org
To: histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
patient tissue being tested, how do you know if it was handled the same as the
patient tissue? For example:
1) Were they processed the same way?
I basically agree with you Glen. I think some people are mixing up
Negative Reagent Controls (substituting negative serum, Ab diluent, etc.
for antibody) and Negative Tissue Controls (substituting a tissue known
to be negative for the antibody being run). It CAN be confusing.
Linda A. Sebree
What??? News to me as well.
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So I just cut a bunch of slides for Ki-7...I am not going to be able to
stain them until Monday. I know the best thing is to put them in 4
degrees, but will it hurt them to stay at room temp for 3 days? Putting
them in the fridge gets a lot of moisture under them and they tend to
lift more. Any
Position Title: Histology Supervisor
Reports To: Laboratory Director
Shift: Monday-Friday, 8am-5pm
Location:
Dermatology Pathology Laboratory for well established busy office in Fort
Myers, FL founded in 2000.
Requirements:
* ASCP certification required
*
They will be safe at RT
René J.
From: sgoe...@mirnarx.com sgoe...@mirnarx.com
To: Histonet@lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 3:01 PM
Subject: [Histonet] Slides for IHC
So I just cut a bunch of slides for Ki-7...I am not going to be able to
stain them until Monday. I know the
I agree 100% with Glen.
Jan Shivers
UMN VDL
- Original Message -
From: Dawson, Glen gdaw...@dynacaremilwaukee.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 1:32 PM
Subject: RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece of tissue as a
I am posting this question for a co-worker. She is attempting to calculate
the cost of producing one formalin-fixed, paraffin embedded block. She has
the amount of personnel time it takes but is having trouble with the reagents,
etc. While we know there are lots of variables she is
Hi Naira,
The short answer is no, it can't be done.
Unless there is a way to release the antibody from the binding site without
damaging the binding site, it can't be done. That said, somehow it should be
possible to find a reproduce-able fix for this kind of issue, by either mending
the
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative control
correct? Then inversely doesn't that mean the same thing towards the use of a
positive control? How can you guarantee the positive
Under separate cover I am sending an article on the subject of direct costs.
René J.
From: Martha Ward mw...@wfubmc.edu
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 3:26 PM
Subject: [Histonet] cost to produce one block
I am posting this question for a co-worker.
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this not to be so. However,
with a negative, the concern is seeing how the patient tissue turns out
I think that this is indeed what is happening here, that there is confusion
between a negative control stain (positive tissue, stained without the primary
ab) and a negative control tissue (tissue known to not express the marker,
stained normally).
I had assumed we were talking about the
Hi Martha,
She is getting different responses because institutions pay different amounts
for their consumables depending on the quantity they use and any vendor
agreements they may have. I broke down my cost by utilizing a years worth of
purchasing information and number of blocks produced for
I can certainly agree with that. Whether it’s the patient’s sample or the known
positive control that are stained without primary, either would fulfill the
purpose of detecting non-specific positive staining.
Jean-Martin
From: Angela Bitting [mailto:akbitt...@geisinger.edu]
Sent:
Thomas,
Agreed, however, how can you say with certainty that the control is still good,
or the antibody is still performing optimally? Hypothetically speaking, if you
had a known positive control and ran it like a patient specimen (positive and
negative) and had staining in the negatively
Pete,
Can't argue with that. I think for the sake of expediency most clinical
services run a known positive and a patient slide for negative. In
the case of H. Pylori, for instance, we may cut a box of control slides
and it's possible to go through the area where the organisms were. This
also
Greetings and Salutations Histoland,
I have a question about denatured alcohol. I work in a government facility
and absolute alcohol (200 proof) is still considered a controlled substance.
This requires a monthly inventory by someone from another department. Years
ago (ok many, many years
Joe, I thought only ethanol, punctilious (fancy word for pure), was controlled.
If it is denatured it should not be controlled.
The 200 proof normally means 100%, but the if the non-ethanol portion is
another alcohol, then it still applies. The key is there is no water in it. I
can't say I've
Curt is right, according to CAP ...
ANP.22570 QC - Antibodies Phase II
Appropriate negative controls are used.
NOTE: Negative controls must assess the presence of nonspecific staining in
patient tissue as well
as the specificity of each antibody. Results of controls must be documented,
No, the best sample for negative control is NOT a section of your positive
control tissue.
The positive control would have already been tested for spurious
cross-reactions. Why continue to test it?
The patient's tissue has not been tested. So a mirror section is used.
Does the patient's tissue
either would fulfill the purpose of detecting non-specific positive staining
- NO
Not in the patients sample unless it is included (which for diagnostic uses is
the most important).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior
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