I’m planning on retiring September 2012 (next year). Our hospital (William
Beaumont Hospital, Royal Oak, Michigan) is currently looking for someone to
co-teach with me for the next year. We train 2 HT and 4 HTL each year. The
program director does all the lectures and labs for histotechnology,
This is so much fun.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting
Sent: Thursday, June 30, 2011 2:50 PM
To: histonet@lists.utsouthwestern.edu; McAuliffe, William
Subject: Re: [Histonet]
MPath Search Partners, a division of Allied Search Partners is currently
looking for a MOHS Histology Technician in Denver, CO.
Schedule: M-F Full Time Day Shift
Requirements:
Previous work experience in MOHS surgery
Summary of Job:
Preparation of tissue specimens
Cutting frozen
I am having a big problem with the HE stained 6 micron frozen sections of
mouse muscle tissue. The Immunofluorescence of adjacent sections on another
slide are fine, but on the HE stained slides the fibers have shrunk and
there are large spaces between the fibers. The sections are from the same
Hello all and happy long weekend (yes our boss gave us the rest of the
day off at 2!!!)
Looking to stain vasculature in tumors. I did trichrome, pentachrome,
and PAS (with digestion) and am being told that these are not optimal
for what they are looking for. Any other ideas to stain blood
Hi Sarah,
You can try CD34, VEGF, and FVIII.
Hermina
Hermina M. Borgerink, MALS, HT, HTL(ASCP)QIHC
Wake Forest University Primate Center
Department of Pathology
Medical Center Blvd.
Winston-Salem. N C 27157
Tel. (336) 716-1538
Fax (336) 716-1515
Email: hborg...@wakehealth.edu
-Original
You are probably looking for capillaries maybe arterioles so there wont be any
elastic lamina for the pentachrome stain to detect.
Instead try IHC with an endothelial marker like VE Cadherin (best) or in a
pinch, CD31 or PECAM.
Jerry Ricks
Research Scientist
University of Washington
So am getting lots of responses for IHC stains (which is ok), but I am
doing these stains on mouse xenografts and the background is getting in
my way a lot. Was just trying to find a simple special that would do
the trick =)
If IHC is the only way, so be it...was just hoping...
What about
Sarah,
if you don't want to go to the hassle of working up antibodies on xenografts,
you could use Ulex Europaeus lectin histochemistry which should work on any
species - great for capillaries
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722
Sarah:
Ronald's suggestion of using Ulex europaeus lectin is a great idea. I second it.
René J.
--- On Fri, 7/1/11, Houston, Ronald ronald.hous...@nationwidechildrens.org
wrote:
From: Houston, Ronald ronald.hous...@nationwidechildrens.org
Subject: [Histonet] RE: ...maybe a little more specific
Hello Histonet,
Please remove my name from the list, as I am on vacation next week. I
will re-subscribe when I return.
Thanks,
Brian Branton
Purchasing Agent
SaraPath Diagnostics
Sarasota Pathology
Sarasota Professional Enterprises II
(941) 362-8963
(941) 362-8964 FAX
I will be out of the office starting 07/01/2011 and will not return until
07/11/2011.
Note: For Cytology issues, please call Wanda at 8-421-5426, For
Histology issues, please call the general histology lab 8-421- 5408, Mario
8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda
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