Hi Mani,
As a fellow KSU researcher I would suggest trying companies such as Dako,
Santa Cruz, RnD Systems, Abcam, and Millipore as they all have distributors
in KSA who can get your antibodies in a fairly reasonable timeframe. From
your list I have only used the Ki67 from SantaCruz, which is alri
In our paper on peliosis hepatis in mice we used peroxidase conjugated wheat
germ agglutinin purchased from Sigma, worked a treat.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
S
Are you asking for suggestions of what steps to take when the water testing
fails? Are are you asking how correct someone who incorrectly did the
testing of the water?
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-Original Message-
From: shehnaz khan
Sent
Some years ago, our deionized water system became contaminated by a gram
negative organism. (I don't know what the bacteria live on, in the dark, with
only deionized water to "eat"). We have a contract with a deionized water
company, but they said it would be impossible to sterilize the whole
Hi,
Does anyone have a good Pan B cell method for immunocytochemistry, to be
used on formalin fixed mouse/rat tissue in paraffin. As its a method I have
never done before.
Kind Regards Sara
-
Confidentiality Notice: This e-mail transmis
I would be grateful to have some advice and detailed protocols from anyone
that works with frozen mouse muscle tissue. I am having a big problem with
the H&E stained 6 micron frozen sections of mouse muscle tissue. The fibers
have shrunk and there are large spaces between the fibers. The
Immunofluo
POSITION REQUIREMENTS:
EDUCATION: High school diploma or GED preferred.
CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American
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certifying agency acceptable to the Laboratory Director – or – exper
I just learned the company making the Brigati Stable DAB is no longer making
it. Does anyone know if someone else has been able to pick up this formulation
and produce it or are planning to make it. We have been using this for over 20
years and I really hate to change. What DAB are others using
We recycle our isopropyl alcohol. We have been told by our microwave
manufacturer that we cannot use the recycled alcohol on the microwave processor.
As a result, I am trying to find additional uses for the recycled isopropyl.
Is there any reason why I can't use the iso in place of ethanol when
Invitrogen - cat# 750118
Sent from my iPhone
On Jul 5, 2011, at 10:46 AM, "Reynolds,Donna M" wrote:
> I just learned the company making the Brigati Stable DAB is no longer making
> it. Does anyone know if someone else has been able to pick up this
> formulation and produce it or are plannin
First of all, if you recycle correctly your 2-propanol there is no objective
reason why you cannot use it in your MW processor, unless your provider wants
you to buy your 2-propanol from them.
René J.
--- On Tue, 7/5/11, histot...@imagesbyhopper.com
wrote:
From: histot...@imagesbyhopper.com
Hi Histonetters,
Does anyone using SAG2Y toxoplasma antibody? if so, can you share with me the
staining protocol?
Thank you!
Chakib Boussahmain
HTL(ASCP)
MIT
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I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature
isn't even lined with endothelium; there's just blood "channels." But most
should be lined.
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.uts
Hello all
I have been following this discussion and I'm going to put in my 2 cents.
Tumor vasculature has traditionally been measured with IHC and CD31 or possibly
another IHC marker.
We use CD31 here for mouse xenograft samples. We have several antibodies we
use and it is dependent upon the
I agree with Liz on most all of this about CD31 and love the Dianova rat
anti ms ab on mouse xenographs, I have also tried the BC rat < ms anti CD31
and it works but not as good as Dianova for the really early vessels.
Another alternative I have used in the past on rats besides F8 and SMA is SC
goa
I have found the following very easy to use (an in-house method):
Principle:
The peroxidase enzyme coupled to the ABC Complex is demonstrated using hydrogen
peroxide as a substrate and 3,3 diaminobenzidine (DAB) as the electron
acceptor. This causes polymerisation of the DAB resulting in a sol
I also agree with Liz's well said synopsis. Be aware that in mouse and human
CD34 is not as "pan" EC as CD31 or VE-cadherin, it is very endothelial bed
selective.
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: "Patsy Ruegg"
Sender: histonet-boun...@lists.utsouthwest
We’ve had several water issues over the years.
First question is – what failed? pH? Bacteria count? Silicates? Conductivity?
Take your readings, and compile a chart showing what the levels are SUPPOSED to
be, what they HAD been the last couple of times (when they were “normal” for
you), and wh
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