[Histonet] Re: antibodies for immunohistochemistry

Hi Mani, As a fellow KSU researcher I would suggest trying companies such as Dako, Santa Cruz, RnD Systems, Abcam, and Millipore as they all have distributors in KSA who can get your antibodies in a fairly reasonable timeframe. From your list I have only used the Ki67 from SantaCruz, which is alri

[Histonet] RE: ...maybe a little more specific

In our paper on peliosis hepatis in mice we used peroxidase conjugated wheat germ agglutinin purchased from Sigma, worked a treat. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com S

Re: [Histonet] corrective action for failed water test

Are you asking for suggestions of what steps to take when the water testing fails? Are are you asking how correct someone who incorrectly did the testing of the water? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: shehnaz khan Sent

[Histonet] Failed water test

Some years ago, our deionized water system became contaminated by a gram negative organism. (I don't know what the bacteria live on, in the dark, with only deionized water to "eat"). We have a contract with a deionized water company, but they said it would be impossible to sterilize the whole

[Histonet] Pan B cells ICC method

Hi, Does anyone have a good Pan B cell method for immunocytochemistry, to be used on formalin fixed mouse/rat tissue in paraffin. As its a method I have never done before. Kind Regards Sara - Confidentiality Notice: This e-mail transmis

[Histonet] Mouse Muscle Fresh Frozen Sections H&E Stained, large spaces between fibers

I would be grateful to have some advice and detailed protocols from anyone that works with frozen mouse muscle tissue. I am having a big problem with the H&E stained 6 micron frozen sections of mouse muscle tissue. The fibers have shrunk and there are large spaces between the fibers. The Immunofluo

[Histonet] FT histotech position in Oklahoma City OK

POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) – or – other nationally recognized certifying agency acceptable to the Laboratory Director – or – exper

[Histonet] Brigati Stable DAB

I just learned the company making the Brigati Stable DAB is no longer making it. Does anyone know if someone else has been able to pick up this formulation and produce it or are planning to make it. We have been using this for over 20 years and I really hate to change. What DAB are others using

[Histonet] Isopropyl alcohol

We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. As a result, I am trying to find additional uses for the recycled isopropyl. Is there any reason why I can't use the iso in place of ethanol when

Re: [Histonet] Brigati Stable DAB

Invitrogen - cat# 750118 Sent from my iPhone On Jul 5, 2011, at 10:46 AM, "Reynolds,Donna M" wrote: > I just learned the company making the Brigati Stable DAB is no longer making > it. Does anyone know if someone else has been able to pick up this > formulation and produce it or are plannin

Re: [Histonet] Isopropyl alcohol

First of all, if you recycle correctly your 2-propanol there is no objective reason why you cannot use it in your MW processor, unless your provider wants you to buy your 2-propanol from them. René J. --- On Tue, 7/5/11, histot...@imagesbyhopper.com wrote: From: histot...@imagesbyhopper.com

[Histonet] SAG2Y antibody

Hi Histonetters, Does anyone using SAG2Y toxoplasma antibody? if so, can you share with me the staining protocol? Thank you! Chakib Boussahmain HTL(ASCP) MIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail

RE: [Histonet] Vasculature in tumors

I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.uts

RE: [Histonet] Vasculature in tumors - long response

Hello all I have been following this discussion and I'm going to put in my 2 cents. Tumor vasculature has traditionally been measured with IHC and CD31 or possibly another IHC marker. We use CD31 here for mouse xenograft samples. We have several antibodies we use and it is dependent upon the

RE: [Histonet] Vasculature in tumors - long response

I agree with Liz on most all of this about CD31 and love the Dianova rat anti ms ab on mouse xenographs, I have also tried the BC rat < ms anti CD31 and it works but not as good as Dianova for the really early vessels. Another alternative I have used in the past on rats besides F8 and SMA is SC goa

[Histonet] RE: Brigati Stable DAB

I have found the following very easy to use (an in-house method): Principle: The peroxidase enzyme coupled to the ABC Complex is demonstrated using hydrogen peroxide as a substrate and 3,3 diaminobenzidine (DAB) as the electron acceptor. This causes polymerisation of the DAB resulting in a sol

Re: [Histonet] Vasculature in tumors - long response

I also agree with Liz's well said synopsis. Be aware that in mouse and human CD34 is not as "pan" EC as CD31 or VE-cadherin, it is very endothelial bed selective. Sent from my Verizon Wireless BlackBerry -Original Message- From: "Patsy Ruegg" Sender: histonet-boun...@lists.utsouthwest

Re: [Histonet] corrective action for failed water test

We’ve had several water issues over the years. First question is – what failed? pH? Bacteria count? Silicates? Conductivity? Take your readings, and compile a chart showing what the levels are SUPPOSED to be, what they HAD been the last couple of times (when they were “normal” for you), and wh