Re: [Histonet] Isopropyl alcohol

Rene, I was just as surprised! When we purchased the mw, we were led to believe that we could use the recycled alcohol, but I was told later that we should not for quality reasons - that recycled is never 100% pure and that water contamination will adversely affect the processing. So, can I

Re: [Histonet] School Program Director Position

To answer a couple of questions that have been asked of me: A) What constitutes teaching experience? Obviously, teaching in a NAACLS accredited HT or HTL program, even if it's only 1 topic. But, it could also include teaching a course at a community college, or some lectures or workshops at

[Histonet] Histology Director Opening

Physician owned laboratory located in Houston TX is currently searching for a Histology Director. Job desc: This candidate will oversee all technical and management duties in the histology laboratory and will be an integral part of the management team. * Responsible for all

[Histonet] Thermo Stainmate

Hi all! I hope you can help me out!  My lab recently obtained the Thermo Stainmate slide stainer with 10 baths.  I was wondering if anyone had a H E protocol for it that worked well.  I have one protocol that I've been using, but the changes require more than 10 baths.  Thanks so much

[Histonet] OT: Wednesday Half-Wit

I used to use Sub-X in the processor. Changing paraffin was no problem because I'd just drain it into a Styrofoam box, allow it to set, seal it up, mark it waste paraffin and put it into the regular trash. Easy, right? Well - then I changed back to using xylene. First time I changed paraffin,

[Histonet] Ehrlich hematoxylin

Hello All I was hoping that someone could answer a question for me. I work in a small lab were we need to make or own ehrlich hematoxylin. We have all the chemical needed to make it. My question is... How long does it normally take the sodium iodate to dissolve and at what point do you add

RE: [Histonet] OT: Wednesday Half-Wit

We used to put a red bio-hazard bag (the heavy duty one's) into a cardboard box. This usually did not leak. Just saying if you still want to use your box method =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744

[Histonet] CBG Recycler Giveaway

CBG Biotech Benchtop Solvent Recycler Model MSLV-03U, has only been used occasionally, free to a good laboratory home. If you would like this instrument, only requirement is that you pay shipping, please send email off-list to: gagn...@kgh.kari.net Recycler product information here:

[Histonet] Slide Labelers

Is anyone else out in histoland having issues with the Leica Slide labeler not printing 'permanently' on their recommended slides. We are using the recommended clipped corner, extra adhesive slides for our HE but every so often the print rubs off after a couple of days. The slides then

[Histonet] 1DH1 Antibody

Does anyone have a working protocol for the 1DH1 Antibody to be used on the Ventana XT or Ultra? Thanks, Mighnon Lashus, HT (ASCP) PathGroup Chattanooga 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 Phone: 423-493-0207 Fax: 423-493-0208 Email: mlas...@pathgroup.com

[Histonet] RE: Slide Labelers

I use a clipped corner slide from Statlab and we do not experience any problems. (IJL-6110) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karlisch, patricia Sent: Wednesday, July 06, 2011 10:32 AM

[Histonet] Looking for Histology work for month of July

Hi all, I am starting permanent job Aug 1, looking for fill-in for month of July. I am located in Orange County California. I could travel as far as San Diego. I am HT ascp and have 17 years experience. Anyone needing a vacation?? Have a wonderful day Netters! Jill Cox, HT ASCP

Re: [Histonet] Slide Labelers

Is your flash tube flashing? Is it old? Spent? We have 6 of them and have never in the 2 years we have had them seen that problem. Stacey Sent from myTouch 4G - Reply message - From: karlisch, patricia pkarli...@hmc.psu.edu To: apos;histonet@lists.utsouthwestern.eduapos;

[Histonet] RE: Slide Labelers

Only if the slide does not make it down to the flash stage Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

Re: [Histonet] Vasculature in tumors

We use CD34 for endothelial staining in blood vessels and Podoplanin (D2-40) for lymphatic vessels. These are for humans only. (Mine was worth less than a half-cent...but hopefully helpful.) :) Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University

[Histonet] Collagen Type VII

Hello Histonet, I'm checking to see if anyone has used (with success) a Collagen Type VII antibody on human tissue for IHC-FFPE sections? We've checked with several vendors and so far all we can find are Col VII antibodies that work only in frozen sections. Thanks! Ross

[Histonet] EM Supervisor job opening soon

We will have an EM lab supervisor's job opening soon at UC San Francisco Medical Center. You can search for it at http://www.ucsfhr.ucsf.edu/careers/ The lab has 3 techs now and does EM of kidney disease, kidney, heart, lung, pancreas transplants, as well as IF for kidney, skin and lung, and

[Histonet] Ehrlich's Hematoxylin

Candice Camille asks about Ehrlich's hematoxylin- The sodium iodate to ripen the hematoxylin should be added last of all, after all the other ingredients, to ripen the stain. (So you will not see if it has dissolved, or not!). Better to ripen the Ehrlich's hematoxylin naturally. Do not add

[Histonet] Re: HT Position - Irvine, CA

This position has been filled. Thank you for your interest. -Eric Velazquez Agendia Inc. On Fri, Jun 24, 2011 at 4:56 PM, Eric Velazquez ervelazq...@gmail.comwrote: Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please

[Histonet] Processing

For large tissue specimens (prostate, placenta, etc.) what temperature do you use for the solutions on your processor? We currently have them all set at 40 degrees Celsius. For paraffin it is set at 60 degrees Celsius. I read the dehydrating and clearing solutions should be at ambient

Re: [Histonet] Processing

Set your formalin at 45ºC, dehydration at room temp with vacuum/pressure and the paraffin as usual. René J. --- On Wed, 7/6/11, Nicole Schuster njbate...@yahoo.com wrote: From: Nicole Schuster njbate...@yahoo.com Subject: [Histonet] Processing To: histonet@lists.utsouthwestern.edu

[Histonet] question on EM

Hi, For those out there that do EM- can you please tell me if you ever just embed and hold a specimen and if so how do you bill it? We just stopped doing them in house and are sending them out and occasionally have a case that is embed only. We are billed with an 88399, but I'm not sure if we

[Histonet] safe handling of tissue for histology containing radionuclides

Hi Everyone, Could someone kindly shed some light on safe handling of tissue for histology containing radionuclides - to comply with CAP standards. How is this done? What should I include in the policy / work instruction? Thanks in advance S Kahn

RE: [Histonet] Processing

We heat from 38-40 degrees for all reagents except the paraffin, which is heated to 60 degrees. I don't use any heat at all on my small biopsies. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] temps and job seekers...

Hi All-   We still need a few really good techs for temp positions. Day and night shift. Also have a number of permanent (regular) job openings to consider.   Happy to give you the fifty-cent tour of traveling and help with a resume--call if you are curious!   Cheryl Kerry, HT(ASCP)   Full

[Histonet] Dpc4 IHC

Is anyone doing IHC for the Dpc4 gene product for adenocarcinoma in biopsy specimens of the pancreas and bile duct and, if so, do you find it useful? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant

[Histonet] Routine H E stain,

Hello Histonetters, I am from toxicopathology lab where we perform HE on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result