Hello,
I'm working on a Zeiss confocal microscope. We use chiefly Alexa488
and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent
protein which can be discerned amongst the Alexa488, Cy3 and Hoechst
33258 emission spectra? I was thinking maybe mBanana?
Thanks
--
Tyrone Genade
You sure can't beat an old AO!
The old Leitz microtomes with the wheel on the front were awesome too.
Ahhh memories.
Sheila HT, ASCP
KDL Pathology
Knoxville, TN
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
hello,
i am working on MMA embedded bone sections and i want to IHC of collagen so
can anyone suggest me the the appropriate antigen retrieval technique to be
used. Any suggestion would be encouraged.please help me out in this regard.
With regards
R.Sravanthi
We still treat the specimens as though they were different specimen parts (A,
B, C, D, etc...) I doubt that the physical arrangement of the containers
affects the billing if you are still treating each core as a different
specimen...
From: Jones, Laura
It might take a Phaldelphia lawyer to figure the multiwell Simport thing out.
However, I am aware that the American Urological Association and conversations
with other pathology leaders advocates treating one container as one CPT 88305.
If you received six cores in one specimen container ,
Do you use a remote alarm on your processor? If so, would you tell me
what brand/model/manufacturer and how it works (keeping in mind that I
am Electronically Challenged)? I run a Leica ASP300 in a new building
with only digital phone lines and zero cell phone reception inside the
building. I
You need to get an autodialer. Search for a company in your area. If you have
Biomed or telecommunications in your facility they should be able to install it
for you. I purchased a land line autodailer from Radio Shack years ago for
three Sakura VIPs. VOIP autodialers are available as well.
We have an alarm system that monitors all of the refrigerators and freezers in
the lab and our processor is tied into that.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original
My apologies, I wasn't ready for the number of responses I received over the
control tissue and might have missed some people. If I didn't contact you back
shoot me another email so I can start working on it.
Control Tissue still Available
Iron/FE
Tonsil
Hpylori
CD15/30
TTF1
p16
AFB
Hello all in histoland. I have a clinician who may be sensitive to formalin.
One of my colleagues suggests that he place his endoscopy specimens into
alcohol instead of the usual formalin to mitigate his exposure. Have any of
you dealt with this problem? What percent alcohol would you
hi all
i'm going to apply for PhD degree in using educational technology in Histology
teaching.
do you know any university i could contact for acceptance letter? i have my
funded scholarship
thanx
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Most clinician I know have other staff in the procedure room. Why not have
someone other than the clinician place the specimen in the formalin. This
way you get the best possible fixation for the patient.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
e-mail
Hi histonet,
We have a brand new Leica CV5030 Coverslipper and I am looking for the
quickest drying media available. We are currently using Micromount by
Surgipath/Leica and it is not drying fast enough.
Any suggestions are appreciated as we have to package our slides for
shipping and the media is
We are shipping them out same day. At this point, if I can not find a
quicker drying media I will have to delay the shipments by one day.
What temperature do you dry them at?
On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk fu...@mercyhealth.com wrote:
Katelin- do you ship the dame day ? We dry
Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you screen first
with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for
the input!
Joanne Clark, HT
Histology Supervisor
Pathology
Our pathology groups decision was to run IHC for H.pylori on all stomach
biopsies.
On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark jcl...@pcnm.com wrote:
Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you
Working on my procedure manual for CLIA and was wondering what the
difference is between QC procedures and QA procedures.?
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If you only need to keep QC records for 2 years, is it the same for QA
records?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of H R
Sent: Wednesday, July 13, 2011 1:30 PM
To:
We only run IHC for H. pylori when the appropriate inflammatory background is
present or when the patient has tested positive in the past and we receive a
follow-up gastric specimen where bugs are not identified on HE.
Running H. pylori IHC on every gastric biopsy is uncalled for and borders on
We run the H. pylori IHC stain on all stomach specimens to improve turn
around time; however, when an inflammatory background is not appreciated by
the pathologist the charge is removed before sign-out and we eat the cost of
producing the slide.
Mark
On Wed, Jul 13, 2011 at 11:37 AM, Richard
Hi Histonetters!!
Here is a an exciting opportunity HOT OFF THE PRESSES!
Anatomic Laboratory Manager Leading Diagnostic Lab Austin, TX
RELIA Solutions the nations only recruiting firm specializing in the
permanent placement of histology professionals has been engaged by a
leading Pathology
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Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma
coffee mugs) will be amused to learn that Cell Marque are giving away packs of
playing cards. Each card shows a section stained with a different antobody (one
of theirs of course, together with the catalogue number).
Its the most powerful treatment Ive ever used!...
http://israelnlp.com/inbox.php?epyahooID=76n4
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How many Hospital-based Pathology Departments with 55,000 surgical specimens,
100,000 PAPs, and 6,000 non-GYN cytology cases have separate managers for
Cytology and Histology? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection
Hello Histonetters,
I was wandering if anyone had a SOP for the collection and processing/staining
of tzank smears that they would be willing to share with me?
Thanks in advance for everyone's help.
Amy Self
Georgetown Hospital System
Dept. of Pathology
NOTE:
The information contained in
Does anyone have a PSLIM/SlideMate procedure that they would be willing
to share with me?
Laurie Colbert
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We run giemsa's on all stomach bx's
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie
[foreig...@gmail.com]
Sent: Wednesday, July 13, 2011 2:29 PM
To: Joanne Clark
Cc:
Quality Assurance outlines all required QC activities used to identify policies
or procedures that are not working as desired, especially those that are not
immediately apparent from following routine QC procedures.
The purspose of a QA plan is effectively achieved by providing a mechanism for
What is the procedure for collecting and processing bone marrow bx? How much
time should it be placed in DECAL??
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I will be out of the office starting 07/13/2011 and will not return until
07/18/2011.
In my absence please ask for Mary . If this is urgent or you need to speak
to me directly you can contact me on my cell phone number 858-472-4266. If
it concerns a Mohs to be scheduled you can e-mail me or
Hi All,
I am doing a survey and will be happy to compile results and share if folks
will respond! What is your favorite antigen retrieval method and/or panel?
Buffer
Source/composition
Temperature
Device
Thanks, Andrea
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Agree, switching to Leica with disposable blade holder changed my life. Never
looked back. Love the CM3050S.
--- On Sun, 7/10/11, Rene J Buesa rjbu...@yahoo.com wrote:
From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] In search of a cryostat...
To: histonet@lists.utsouthwestern.edu
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