Re: AW: [Histonet] Quick Drying Mounting Media

Have you tried drying them under a blow dryer? That's how we used to dry our slides before we sent them out. It was a commercially available slide dryer. Michelle Sent from my iPhone On Jul 14, 2011, at 3:57 AM, Gudrun Lang gu.l...@gmx.at wrote: I think it's more depending on the organic

Re: [Histonet] And now, IHC playing cards!

Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, mtitf...@aol.com wrote: Those of you

Re: [Histonet] And now, IHC playing cards!

Hello again! I went to their site and can't find anything...hopefully I didn't miss it!! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, mtitf...@aol.com wrote:

Re: [Histonet] Antigen retrieval survey

Citrate buffer René J. --- On Wed, 7/13/11, Andrea T. Hooper andreahoo...@rocketmail.com wrote: From: Andrea T. Hooper andreahoo...@rocketmail.com Subject: [Histonet] Antigen retrieval survey To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 13, 2011, 11:10 PM Hi All,   I

[Histonet] Re: Histonet Digest, Vol 92, Issue 18

On all stomach biopsies. Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18 Send Histonet mailing list submissions to

RE: [Histonet] Antigen retrieval survey

I hate to be pessimistic, but different clones of monoclonal antibodies can have different optimum retrieval requirements, not to mention tissue type, source species, etc. What I do believe however, is that enzymatic retrieval is under-sold - no equipment purchase is necessary so you will

[Histonet] tissue processing problem

We have several tissue blocks with cells that have shrunken. The pathologist called them shrink o cytes. The tissue affected is mostly small gastric polyps. This sounds like a dehydration issue. Any suggestions would be greatly appreciated. Thank you, Carol Carol Bryant, CT (ASCP)

[Histonet] New Position Alert - Field Histology Southern CA

Good Morning Everyone, New Position Alert!! One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist

[Histonet] Antigen Retrieval

Citrate buffer is my first port of call. Jim Reilly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] tissue processing problem

You are right, it is most likely a dehydration issue. Try to start with 70EthOL and increase gradually until 100EthOL René J. --- On Thu, 7/14/11, Carol Bryant cb...@lexclin.com wrote: From: Carol Bryant cb...@lexclin.com Subject: [Histonet] tissue processing problem To:

RE: [Histonet] Antigen retrieval survey

96 degree water bath for half an hour in either citrate buffer (pH 6) or Tris-HCl (pH 9), depending on which gives better retrieval for that antibody. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] Autoclave water

Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers

[Histonet] Re: IHC for H. pylori

The three pathology services I've worked in in the last few yers that did immunohistochemical staining for Helicobacter pylori did it on all gastric biopsy specimens. Usually R/O H.p. is on the requisition. I prefer a blue stain (Giemsa or Diff-Quik II or equivalent) if I have one slide a day to

Re: [Histonet] Autoclave water

Same with deionized, filtered water? /daniel On Thu, Jul 14, 2011 at 5:51 PM, sdys...@mirnarx.com wrote: Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave.

RE: [Histonet] Autoclave water

Have you tried glass lids? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Sjolander Sent: Thursday, July 14, 2011 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autoclave

RE: [Histonet] And now, IHC playing cards!

I emailed my rep and asked her to send me some. I'm studying for the QIHC and told her it would be a good study tool. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email:

[Histonet] Lab humidity range

We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab

[Histonet] P16

What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have

[Histonet] TTF-1

Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform on the Benchmark XT? Our pathologists have read this clone is supposed to be more sensitive than Ventana's 8G7G3/1 clone Thank you Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message,

Re: [Histonet] TTF-1

My lab uses the SPT24 clone for TTF-1. Let me know if you'd like any protocol info. Mark On Thu, Jul 14, 2011 at 11:47 AM, Inman, Anna anna.in...@stmarygj.orgwrote: Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform on the Benchmark XT? Our pathologists have read this

[Histonet] RE: Lab humidity range

We chose 10 - 90% as our range after reading what few instrument manuals even addressed the issue. I can only wish we could get to 17% humidity here in NC. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center

[Histonet] Humidididity Ranges

I love your replies because it gives me quite a range in order to avoid Incarceration, but I need to know THE CRITERIA (specific equipment operating parameters, for example?) you used to come to that range. I'm walkin' the plank here and I'm getting close to the end... Sally Breeden, HT(ASCP)

RE: [Histonet] Histobath revised

We continue to use the Histobath, but the istrument is no longer being produced. I suggest you search the instruent resellers for a unit. We have had to move to the Hacker-Bright Clini RF, a floor model and , of course, the cost is significantly higher. William DeSalvo, B.S., HTL(ASCP)