Re: [Histonet] Microtome draft shield

Dear Keri, Our Bright M3500 microtome is fitted with a clear retractable visor over the specimen and knife area that will more than likely solve your problem. Please take a look on www.brightinstruments.com Alan Bright Bright Instrument Co. Cambs England Sent from my BlackBerry® wireless

RE: [Histonet] Microtome draft shield

Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of

[Histonet] ER/PR

Hi All, I would like some input on ER/PR by IHC. We have a lot of problems with tissue detachment from the slides. We use a DAKO autostainer and do the heat retrieval in the Pascal pressure cooker. We use high pH retrieval buffer and heat to 120 degrees and hold for 1 minute, but when you

[Histonet] RE: ER/PR

We heat ours to 99C for 20 minutes with a cool down. Using the Dako PT link. (water bath) BUTbefore you change your pretreatment Make sure that your tissue is fixed properly, processed properly. After it is cut, make sure that the slides dry before you baked them in the oven. Any water

[Histonet] C FLIP AND NKF Beta antibodies

I have been asked about a research project involving these two antibodies and we are having some trouble finding sources that are appropriate for formalin fixed, paraffin embedded tissues. Does anyone have experience with these antibodies? Martha Ward, MT (ASCP) QIHC Assistant Manager

[Histonet] locums pathologist survey

Dear Histonet, I was wondering if anyone in histoland can tell me what your experience has been with a locums pathologist. Cost? And how was the service? My understanding is that they can cost around $2500 per day PLUS expenses. Thanks in advance, Andrew Andrew Byrnes www.AccelPath.com

[Histonet] Evening Histotech Position Opening

Hello All, I have a full time evening position available for a registered HT or HTL in Spokane Valley, WA. The hours are 5:00 p.m. to 1:30 a.m. M-F. InCyte Pathology is a great company; it was voted one of the top 100 Washington Best Companies to work for List in 2011. Check out the website

[Histonet] Assisting with Autopsies

Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility

[Histonet] RE: Assisting with Autopsies

We have a diener and residents who help and our PA as well. We do about 40 a year. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To:

[Histonet] RE: Assisting with Autopsies

Although our autopsy volume is also low, our two PA's take turns assisting. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To:

[Histonet] RE: Assisting with Autopsies

We are small too, about 3-5 per year and that is one thing I hate...yes we do. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To:

RE: [Histonet] Assisting with Autopsies

Hi Amy, We do about the same number of autopsies per year... Only on in-house patients and they have to be physician requested, no family requested. We used to hire a diener to come and do them and the techs would assist. None of the techs cared to do that, so I usually volunteered, since I

Re: [Histonet] Controls with patient specimen on same slide

Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a C or P written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni

RE: [Histonet] Controls with patient specimen on same slide

Whenever possible we put patient tissue on slides with positive control tissue on it. Everyone likes it and then you're assured your control and patient got the same staining experience. We used to use the red box control slides but discontinued due to the additional cost. Linda A. Sebree

RE: [Histonet] Controls with patient specimen on same slide

We often do the same or use separate slides. We used to use the slides with the designated control area but had some problems with the reagents getting to all areas of the slide. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: Controls with patient specimen on same slide

We do that. Not only does it save the cost of an extra slide, it assures that both sections are treated the exact same way. Our pathologists are very happy with it. We don't use the expensive slides, we just always put the control at the top. Best, j Joyce Weems Pathology Manager Saint

[Histonet] er pr validation

I know this has been talked about before here but how many slides are people doing for er pr validation, and are you doing it with another hosp? thanks so much for your help. anita dudley providence hosp mobile al

Re: [Histonet] er pr validation

Hi Anita, We ran 40 cases and sent the same blocks to Phenopath for them to run for comparision. There is a requistion for doing an ER/PR validation on their website. Mark On Tue, Jul 19, 2011 at 1:43 PM, anita dudley azdud...@hotmail.com wrote: I know this has been talked about before here

[Histonet] Re: ER/PR

I don't work with these antibodies but when we were doing bone marrow bx pressure cooker, high pH retrieval we lost a lot of tissue. This was what we had previously work out for our antibody. We replaced the pressure cooker with a 70ºC water bath, kept the high pH retrieval and cooked for 2

Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections

RE: [Histonet] Controls with patient specimen on same slide

We used to do batch controls too. And we cut unstained slides in a similar fashion also. In order to put the control on the already cut slide, one must stand on one's head or hang themselves upside down from the rafters, but it can be done.. Just put the slide in the water label end first and

[Histonet] Re: locum tenens pathologist survey

Andrew Byrnes at www.AccelPath.com asks: I was wondering if anyone in histoland can tell me what your experience has been with a locums pathologist. Cost? And how was the service? My understanding is that they can cost around $2500 per day PLUS expenses. Well, having been in locum tenens

[Histonet] Temps needed --call if curious? WITH phone number

Blank email--sorry!  User glitch.   Okeydoke--what do you want to know?  Fifteen minutes on the phone will get you the following:   --the good vs. bad of traveling (yup--kinda like most other jobs) --how it works (and doesn't work) --who pays for what and what will I make? --what happens when I'm

[Histonet] Laura Miller is Out of the Office.

I will be out of the office starting 07/19/2011 and will not return until 07/20/2011. I am out of the office. I will return on July 21st. I will reply to your message on Thursday __ This email has been scanned by the