negative pressure will make the ribbons have a flying party. is it feassible
to built a room within the lab with no pressure for sectioning?
mg
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hi Histonetters!
I'm looking for thoughts on preferences/pros/cons between using a progressive
and a regressive HE on routine daily work.
Which hematoxylins do you prefer (commercially prepared), which eosin?
Anyone have a tried and true protocol for each method?
Thanks!
Michelle
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Are the disposal issues over the
Used paraffin because of chemicals like xylene in the wax
Or is it the human tissue in the
Wax
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We use Mucicarmine placed in a small dropper bottle, it does not wash
out in the processor and makes the tissues easy to see once embedded.
Lisa White, HT(ASCP)
Supervisory HT
James H. Quillen VAMC
PO Box 4000
Corner of Veterans Way and Lamont
PLMS 113
Mountain Home, TN 37684
You do not get 100% alcohol back from recycling, so it cannot be used as such
in either the processor or stainer. Basically, you have to use it as 95%.
That may be your problem!!
This e-mail and any files transmitted with it are confidential and are
Hi all,
We dump everything (and I mean EVERYTHING) down the drain. Formalin,
waste from Benchmark, etc.
We use 95% recycled alcohol for everything as well (stainer, making up
reagents, etc), but that's the only reagent (other than clear-rite) that
we use recycled. Our 100% alcohol is right
Our paraffin is also hauled off with hazardous waste
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 0216, Fax 812-482-0232,
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
I have a protocol I will share from the benchmark lt for dual stains if you
want to e-mail me directly!
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 0216, Fax 812-482-0232,
Pager 812-481-0897, Cell
Hello Histoneters,
I'm having problems getting a good section on paraformaldyehyde fixed
adult mouse eyes frozen in TBS tissue freezing medium. One of the
problems was the orentation of them. They were too close to the edge. I
also, wanted to try a harder freezing medium, so I corrected oriented
Try Neg-50 (Fisher, cat#22-110-617) instead of OCT, we don't have any problem
with fresh-frozen mouse eyes.
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com
Progressive hematoxylins usually take more time to stain and for routine work
the schedule has to be very fine tuned because otherwise obtaining the ideal
staining is quite difficult with a frequent tendency to the under staining.
Regressive hematoxylins on the other hand, always over stain and
FOR
BENNCHMARK LT
Procedure XT IHC DS UDAB-URED
Depar
Cell condition short 8 min
37 degrees/default
Mart 1 32 min
Amplify
ultrawash
aby denaturation
90 deg C 4 min
37 degrees/default
Ki67 4min
DS ultra wash
counterstain Heme (we do 20 min on this our Path likes it dark)
usually Heme is 4
Hi Marc and Histonetters (especially those using XTs),
I've been trying to work up VMS's new EBER probe with less than stellar
results.
I have some questions about the protocols you sent via Histonet back in
early June. My questions/protocols are in red.
You state the following:
Depar
Hi Linda,
I had simliar trouble before I bought and added the extra HybReady to the
protocol (there is one that comes with the detection kit, but you need two
for good staining). It will clean up your stain and A LOT and remove those
blue splotches that show up when you don't use it.
Mark
On
Just wanted to add a few details.
We are currently searching for a Cana date for our opening. We are
located in Northern Arizona in the pines. We have two Campuses with the
main campus in Prescott with the second campus located in Prescott
Valley. We are a total of Approx. 187 beds combined.
Hi all,
I had a very difficult time cutting my very precious triple transgenic
frozen decalcified mouse bones this morning. As I cut into them, the
sections scrunched up into a mess of OCT pretty much as soon as the blades
hit the block. These were 4% PFA perfused and post-fixed for 24 hours,
I would like some thoughts on how to resolve some blurry looking tissue. We
have had occasional tissue that looks blurry and not crisp for several weeks
now. It is not all the cases only random tissues. The tissue is not on the
same tissue processor either. We have 2 processors. The latest
Hi Everyone!
I was wondering if anyone out there had and was using the Biogenex Xmatrx
Infinity Stainer from Biogenex. We are considering buying one to do ISH and
FISH on, but was wondering what other people had to say about it. Any
feedback would be greatly appreciated!
Thanks!
Anna
I was a little distressed to read the message from Amy in Camp Hill,
Pennsylvania declaring she dumps everything (and I mean everything) from her
histology lab down the drain. There are a bunch of Federal Laws governing
handling and disposal of chemicals used in the histology laboratory and
Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:
Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII
Also could use a suggestion for C-kit in Dog tissue only.
thanks
Mark
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Anyone using this system, would you please contact me off line? I have
questions regarding the PicsPlus component as well as I'm curious to see how
everyone likes it.
Thanks! j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 -
Hi Mark,
Vimentin - Dako; M0725, clone V9
Pankeratin - Dako; M0821; clone MNF116
CD3 - LabVision; RB-9039; rabbit polyclonal
CD20 - LabVision; RB-9013; rabbit polyclonal
CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore);
clone CA16.3C10
CD18 (feline) - Leukocyte Antigen
What is the staining intensity like?
Thanks,
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: nacaela.john...@usoncology.com
-Original Message-
From:
Hello Mark
We use the Ventana Benchmark for most of our IHC. We don't use all of
the markers you listed, but and for our canine and feline tissue we do
have great results with the following:
Vimentin-Ventana
Cytokeratin-Dako
CD18-UC-Davis (Dr. Peter Moore)
C-kit-Dako
We worked hard to get the
Hello-
Is there anyone in the southeast part of the country with familiarity with the:
Shandon Hypercenter XP tissue processor?
Leica ST5050 IHC stainer (Jung Immunostainer)?
I was looking for someone with experience with either of these that may be
interested and able to pass along some
Michael,
Since this seems to come up somewhat regularly, if you do have a link to the
federal laws which govern this, maybe you could share. I'm sure this would help
labs get the support they need for proper disposal.
Toni
-Original Message-
From:
Hi.
Does anyone out there have a protocol for CD24 and CD133 that will work on
mouse
Xenograft tumors? Preferably a protocol that will work on Leica Bond, but any
working protocol will do at this point.
Thank you and have a good day.
Dusko
___
We are trying to validate a Biocare decloaker and have found when we use
122 degrees for 30 seconds we get great signal, but distorted
morphology.
If we reduce to 90 degrees for 45 minutes, the signal is significantly
decreased but the morphology is good.
What protocols are you using?
One should not automtically assume that laws are broken here.
(Rant begins here)
First of all, it is the States that set the limits of what can and
cannot be dumped. All States must meet Federal standards,but States are
free to determine how they do that. (It's one of the benefits of the
I may not be able to Like Bill's rant, but I second it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, July 25, 2011 4:20 PM
To: mtitf...@aol.com;
Good Afternoon to All,
I am getting pretty desperate to find a source for Pneumo control slides. I
know they are hard to come by, but my favorite source Newcomer Supply does not
have any and I ordered some from Sigma-Aldrich and now they are discontinued.
Please Help!
Thanks,
Wanda
WANDA
There is a huge difference between 122 degrees for 30 seconds and 90
degrees for 45 minutes. I would say you need to look at something here!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
As to the efficacy of retrieval, there is actually not that big of a
difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent
to 125 degrees for 30 seconds.
My guess, based on limited information in the original email is that Diana
needs a new gasket. Those should be
I wonder if a new gasket would help me.. I keep blowing mine! :)
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
You just pointed out to the most likely causes: happening during staining, high
humidity and water in the xylene. Try to take care of these issues and you will
resolve the problem.
René J.
--- On Mon, 7/25/11, Carol Bryant cb...@lexclin.com wrote:
From: Carol Bryant cb...@lexclin.com
Subject:
Ok that is a bigger problem and a very clear answer to your staining issue.
Your decloaker should never blow a gasket. Something is wrong and you
probably need service or a replacement. Your decloaker is probably getting too
hot and over retrieving tissue. I suggest immediately calling Biocare
Your rant is interesting but wrong.
OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous
materials down the drain.
I was also taken aback by Amy's posting.
No, regardless of what your state law may or may not permit you to dump in the
drain, you should not put some $avings
I totally agree, under any circumstances, whatever the State law defines, we
should not dump formalin, xylene or alcohols down the drain. And even though
California has very strict rules, it even seems to differ from county to
county. In one of my past jobs we were allowed to dump the formalin
American MasterTech sells them:
http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesite
m=CSP015P
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
wanda.sm...@hcahealthcare.com
Sent:
Indeed not only county to county but your local municipality can have specific
regulations related to hazardous waste discard in the sewer since it is their
waste treatment plant that takes the hit so to speak. Most small
municipalities will adopt state or county regulations (whichever is
This sounds to me like a processing issue, especially with the types of tissues
you describe. Are the tissue sections you are referring to very thick? Maybe
the tissue is too big for the amount of time in the different processing
stations. I have also seen this happen when the tissue has
American Master Tech.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
wanda.sm...@hcahealthcare.com
Sent: Monday, July 25, 2011 1:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Where Can I
Statlab sells pneumo controls and they are in human tissues.
Thanks
Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010 x229 | Fax: 972-436-1369
dsi...@statlab.com | www.statlab.com
- Original Message -
Hi Michelle
I have used both and my experience either is suitable for a good HE if they
are used appropriately. For regressive staining you just need to optimise the
time of haematoxylin staining with the concentration and time of the
differentiation step.
In a previous position I used
Hi folks,
We were hoping someone could recommend a temperature range acceptable for
storing tissue blocks? Also do you folks use a regular thermometer or a
temperature chart recorder (records on graph) to record room temps?
We are going to move to a new location and need help with the fine
Please unsubscribe me
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