Dear fellow histonetters,
I would like to have your suggestions regarding trimming and sectioning of
mouse heart. Our pathologists are interested in evaluating mouse ventricle
papillary muscle for some lesions. I am not able to cut sections having
papillary muscle in all sections.Some
Dear All,
Our lab is in the process of purchasing our automated tissue processor
(Peloris Rapid tissue processor).
I would really appreciate comments from anyone who really likes, or
dislikes the processor that they are using.
Thanks in advance!!!
Muhammad
Muhammad:
The only thing I dislike about the Peloris technology (it is a technology in
itself) is that after the dehydration with 2-propanol, the tissues are
subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the
infiltration step with melted paraffin.
That step of drying
Treat the tissue exactly as if it was human.
Sure, your anti CD68 may not work on pig but, it's worth a try.
LBH? Probably LDH. If so, better to measure serum levels?
Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests.
So, it seems that 2 and 3 are not IHC at all.
More
I am out of the office until 08/15/2011.
I will be out of the office Thursday 8/11 Friday 8/12, returning Monday
8/15, please call 1800-225-3035 (leica customer service) with any urgent
matters, or email lisa.raym...@leica-microsystems.com. Please have your
account number and PO available.
Carl is correct... treat the tissue as if it is human... i.e., use
anti-mouse IgG as your link secondary Ab for CD68 mouse primary antibody,
etc. Not all CD68s cross-react with non-primates; I can let you know which
clones I've tried when I get back into work on Monday.
Jan Shivers
IHC
