[Histonet] cd4 from angio proteomie

Hello, Has somebody used the CD4 antibody from Angio proteomie. It is an anti mouse and it is for paraffin IHC. Catalog # 2H9 Is it working? Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht University visiting address: Universiteitsweg 99, room

RE: [Histonet] Online program for histotechnician program

We have an HT trained at our site while attending Harford, great end result. :) Caroline M. Pratt, MBA Practice Administrator Dermatopathology Penn Medicine 3020 Market Street, Ste 201 Philadelphia, PA 19104 cell 610-800-1381 phone 215-349-8178 fax 215-662-6150 -Original Message-

[Histonet] glass and humidity

Has anyone ever done any sort of test/experiment of the effect humidity might have on slides? Last summer we had an issue with inconsistent staining with our IHC and we finally attributed it to the positive charge on the slide being uneven somehow, causing reagents to pool in some areas on the

[Histonet] pten Antibody

Can anyone recommend a pten antibody for use in FFPE? Antibody I was using is no longer available Thanks Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 luis.chirib...@nyumc.org /PRE html body

[Histonet] LKB 7800B KnifeMaker

Does anyone have some quick tips for adjusting a knife maker, specifically a LKB? We have the guide that came with the equipment. However, it's not very clear (to me) how to adjust the alignment based on what the guide says. It just seems to be an index of sorts without very much advice on how

[Histonet] Toxoplasma control

Hello all, Does anyone have a Toxoplasma control they would be willing to share? I would even be happy with a few unstained slides if an entire block isn't available and would be willing to trade control tissue from my lab too. Thanks in advance. Margaret G. Coppin, HT(ASCP)

[Histonet] RE: pten Antibody

Leica sells the NovoCastra line pTEN. That's what I have been using. Which vendor have you been using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Monday, August 15, 2011 10:41

RE: [Histonet] mouse heart trimming and sectioning_papillary muscle

You are probably going to have to check unstained sections as you trim into the block. The heart needs to be grossed in from apex to base in a standard manner, you will need to figure out the best way to do this. You need to section on the same orientation each time. I'm thinking you need to

[Histonet] Cassette Labeler

We currently have a Thermo Fisher Printmate cassette labeler. I am looking for something smaller than this to put in an outpatient surgery center that will be opening soon. It needs to be able to print the accession number and a corresponding barcode on the cassettes - and it needs to be easy

[Histonet] Cytochemistry staining - reference lab

I'm looking for a reference lab in the Los Angeles area that does cytochemistry stains - specifically a TRAP stain. Does anyone know where I could send tissue sections for this stain? Laurie Colbert (626) 397-8620 ___ Histonet mailing list

[Histonet] protocol for in situ RT_PCR

Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list

Re: [Histonet] LKB 7800B KnifeMaker

It is an alignment problem. Also chipping at the scoring line indicates a too high pressure of the scoring wheel onto the glass (there is a set screw to loosen the pressure next to the scoring wheel, you have to remove the head to adjust). I have an instruction sheet with drawings to align the