Dear Sir,
The Clin-RF Rapid Freezer for specimen preparation is part of a range we
manufacture and sell through distributors in most regions of the world. The
Clini-RF freezes down below -80 deg C using the latest non inflammable fluids
or rapid conduction system.
Best regards
Alan Bright
Hi Rene,
I am also interested in your article - could you send me a copy or provide
the reference?
Thanks,
Andrea
Andrea Marion
Graduate Student
University of Illinois at Chicago
Rene J Buesa rjbuesa @t yahoo.com
Sun Sep 25 10:36:24 CDT 2011
Previous message: [Histonet] Block age/labile
An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI. Their work shows that the loss
of antigenicity in archival formalin-fixed, paraffin-embedded tissue
sections is due to the presence of water, both endogenously (poor
processing) and
I am looking to purchase a glass coverslipper in the near future (to coverslip
approx. 1600 slides/day) and would like references from any of you that have an
opinion (positive or negative). Our slides have Ventana labels on them.
Thank you, Ann
We have the Sakura Glas coverslipper and we really like it. We still have some
problems when it gets really humid in the lab and the coverglass will stick
together, but overall it seems to work well for us.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box
Does anyone have a pdf of this that they are willing to share.
Thanks
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway
Dear Adam,
Thank you for the kind words. It has always been a pleasure to pass histo
information onto others to help them with their projects. As for dinner
and a drink, I would take you up on that - hopefully with Andrea there too.
In lieu of that, I raise my morning cup of coffee to you
Adam,
I will miss your contribution on the Histonet. You have always been an asset to
the listserv. I agree with your sentiments exactly, I know what I know because
of the information I learned on here and from those kind souls willing to
share. I continue to try to pay it forward and not get
Hi Histonetters!
I hope you all are having a great day. While I usually work exclusively
in permanent placement one of my very best clients has asked me to put
feelers out on their behalf because they are in need of a histotech for
a 3 month temporary assignment. This is full time morning shift
Ditto on the Leica CV5030.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org
-Original
I second that.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I need to move my HPV 6+ and HPV 16+ from my baby BenchMark to my BenchMark XT.
Is there anyone willing to share their protocols?
Thanks.
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Histonet@lists.utsouthwestern.edu
I am reposting this in case it was missed earlier. The Stowers Institute is
looking for someone with a solid background in Histology, including research
histology and supervisory experience to head the Histology and Electron
Microscopy Core Facility. I will post the actual ad below, but before
I am posting this again to see if anyone has worked with nonstructured
proteins. Do they form the same crosslinks when fixed with formalin? Would
antigen retrieval be of any use? Do they react with IHC the same as structured
proteins?
Margaret Perry HT(ASCP)
Dept of Veterinary and
After a short look into wikipedia I assume, that these proteins can also
bind formaldehyde as long as there are aminogroups of lysin and arginine
reachable. Crosslinking would be possible, if bound methylgroups come near
enough to peptidbinding. If this occurs intra- or intermolecular, who knows?
Hi all,
we are a histo lab doing about K14/year. There are 5.5 histotechs and six
pathologists. The grossing is done by one PA assisted by one tech aid.
Biopsies are processed on the microwave processor everything else gets done
overnight. Pathologists start getting slides at 9AM and have the last
Hi all,
we are a histo lab doing about K14/year. There are 5.5 histotechs and six
pathologists. The grossing is done by one PA assisted by one tech aid.
Biopsies are processed on the microwave processor everything else gets done
overnight. Pathologists start getting slides at 9AM and have the last
We use H.Pylori from Cell Marque and the Benchmark XT as well. When we began
doing the H.Pylori by immuno (versus Giemsa) we had had the same problem. Tech
support from Cell Marque suggested using a Protease 2 (from Ventana) to decrese
background, and it worked. Here's our protocol:
CC1
Does anyone have an e-mail address for Dick Dapson, Clive Taylor, and William
Grizzle?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
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Histonetters...
Would anyone performing ISH probes for Kappa Lambda on the BOND III be
willing to share their protocol for B+ fixed, decaled, paraffin-embedded bone
marrow core sections. I have tried a variety of different combinations and
times with ER1, ER2, Enzyme incubation, yielding
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