RE: [Histonet] P 16

2011-09-30 Thread Hoekert, W.E.J.
Hi Phyllis, We are doing a double stain (Mib-1 - P16) on cervix biopsies in which the P16 antibody is coloured with AP. It works fine, our pathologists are quite fond of it. Almost every day we have a few slides. Willem Hoekert OLVG, Amsterdam The Netherlands

[Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

2011-09-30 Thread Steve McClain
The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

2011-09-30 Thread Yaskovich, Ruth A (NIH/NIDCR) [E]
Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

2011-09-30 Thread Amber McKenzie
What about Alcohol formalin from the processor? The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that down the drain with water? And, what about the HE stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain?

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

2011-09-30 Thread Galbraith, Joe
Amber: Actually, no we do not dump the items you listed down the drain. They get recycled appropriately. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, September 30,

[Histonet] Alignment tool

2011-09-30 Thread jsjurczak
Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

2011-09-30 Thread Connolly, Brett M
We collect EVERYTHING for recycling or disposal by our site-wide contractor http://www.cleanharbors.com/ Only environmentally approved hand soap and glassware cleansers are allowed down the drain, along with water. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box

RE: [Histonet] Alignment tool

2011-09-30 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
Newcomer sells one Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday,

[Histonet] RE: KP Markers!

2011-09-30 Thread Adam Harris
If you would like to obtain KP Markers, they are now being carried by Sensor Health, inc. in Cambridge, Ontario, Canada. If you have any questions you can contact Adam Harris at 888-777-7080 or 519-241-2194. You can check out their website at www.sensorhealth.com

RE: [Histonet] Background in FFPE glandular tissue sections

2011-09-30 Thread JMyers1
Mesruh: The easiest method to address such is a problem is to apply a serum-free (e.g. casein-based) protein blocking agent, which can be obtained from a variety of vendors. This technique is particularly effective when the reagent is allowed to incubate with the specimen for 5 to 10 minutes

RE: [Histonet] Alignment tool

2011-09-30 Thread Sarah Dysart
I actually just got a flyer for one of those from American Mastertech that looked pretty neat? http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=EQS05 Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas

RE: [Histonet] Alignment tool

2011-09-30 Thread Bernice Frederick
So does TechOne biomedical Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From:

[Histonet] Control Tissue

2011-09-30 Thread TerryStar Bullard
Hi all. Does anyone have a resource for control blocks? We are in need of fungus controls and are trying to save the cost of buying slides. Thanks all. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc Sheila, National Society for Histotechnology has a Tissue Control Bank, If you

[Histonet] Position Available @ NIH (Bethesda, MD)

2011-09-30 Thread Jason Blaine
Seeking highly skilled scientist or histologist who is well versed in IHC. This position is in the National Institute of Mental Health (NIMH) and thus requires prior neuroscience and non-human primate experience. Please note that we are NOT necessarily seeking ASCP histology certificated

[Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the drain

2011-09-30 Thread Steve McClain
Yes nothing down the drain (except maybe the lithium carbonate bluing). Hematoxylin goes into medical waste service. Used Eosin gets reused a 10-20 ml at a time into the first absolute on the processors as a marker of carryover (as the later alcohols turn red, we have a visual indicator of the

[Histonet] washing the placenta

2011-09-30 Thread An Eerdekens
Dear all, Is there someone with experience in washing of placentae before sampling? Regards Dr An Eerdekens ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet