Re: [Histonet] Difference between Neutral Buffered Formalin 10% andNB formaldehyde 3.7 to 4%

2011-10-05 Thread joelle weaver
I considered mentioning the gas state of formaldehyde, and its saturation potential in aqueous solution, so I am glad to see this was posted.. However I was hesitant to reply right away b/c I too was sad that you were given this misinformation, especially since it is so easy to look up and veri

[Histonet] (no subject)

2011-10-05 Thread Janice Mahoney
Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster p

Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

2011-10-05 Thread William
Jenni, we do not "oh dear" you, in fact we congratulate you for coming here to get the answers you need. Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid. 100% Formaldehyde is a gas. To use it in histology we purchase it as formalin which ha

RE: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

2011-10-05 Thread Rosie Scrimizzi
concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.same thing.. yes..oh dear!! > From: cha...@yahoo.com > Date: Wed, 5 Oct 2011 16:05:31 -0700 > To: histotech...@gmail.com > Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and > NB fo

Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

2011-10-05 Thread William
Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > thing and in the book from Frieda Carson

[Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

2011-10-05 Thread Jenny Vega
Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutr

[Histonet] Isotype specific polymers

2011-10-05 Thread Milne, Katy
Hi everyone, I'm curious if there is such a thing as isotype specific polymers (ie will react with mouse IgG2a but not IgG1). Does anyone make such a thing? Thanks, Katy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouth

[Histonet] Labvision Autostainer

2011-10-05 Thread Jones, Laura
Good afternoon fellow Histotechs! We have been using our Labvision Autostainer for 5 or so years now, and love the open system. Recently, we had a rep from Fisher come and do a PM on it, because we noticed the amount of reagent in some bottles was not changing at all, while others were running

[Histonet] Skin punch biopsy

2011-10-05 Thread Hughes, Anna
Hi! I have 2mm skin punch biopsies that I need to process, but it has been a very long time since I have done this and don't really remember the times/station. These biopsies have been cut in half so they are extremely small - In other words, I am only planning on processing 1/2 of the biopsy

AW: [Histonet] Paraffin sections for molecular assays

2011-10-05 Thread Gudrun Lang
Hi! For our KRAS-testing we clean the microtome with LTK008, especially surfaces that come in contact with the sections. We use one-way-toothpicks, single packed, to pick the slide from the blade and give it directly into a sterile collection tube. 1-10 sections are collected depending on the dimen

Re: [Histonet] Ann Preece (was "decal [sic] question")

2011-10-05 Thread DKBoyd
I have both a 1959 and 1972 copy of Preece's book! Both were left by the previous Histology Manager. What a treasurer for me. I also used Preece when studying for the registry. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l P

[Histonet] "decal [sic] question"

2011-10-05 Thread Louise Renton
funny, I thought a decal was a sort of transfer for decorating things with - like guitars and hot rods - what others call "stickers" hard to trademark a word like that! On Tue, Oct 4, 2011 at 5:38 PM, Bob Richmond wrote: > Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology > C