[Histonet] In Newfoundland Canada the scale is $32-42 / hour.

In Newfoundland Canada the scale is $32-42 / hour. Elizabeth - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this

[Histonet] Spirochete Control Block

Hello Histonetters, Anyone have a spirochete control block(s) they would like to trade? Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Automated coverslipper with Ventana labels

In my experience with the Leica coverslipper, I have to hand coverslip all Ventana slides. It throws more than it coverslips. I have tried running them down quickly and avoiding Xylene on the labels, but that works only marginally better. Ashley Troutman BS, HT(ASCP) QIHC

[Histonet] mast cells for arteries

Does anyone have a go to stain for Mast Cells besides Tol Blue? We are trying to demonstrate the presence of mast cells in human arteries ffpe. Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use

[Histonet] Leica coverslipper and Ventana slides

I use histoclear in the last step of dehydration and have no problem with the arm picking up the slides. If I leave the slides for more than one hour in the last histoclear and if the histoclear covers the labels, then the glue of the label dissolves and interferes with the arm picking up the

[Histonet] HISTOTECHS ARE NEEDED IN TEXAS

I have an opening position for histotech.This is a full time and permanent position; if you are interested please call me or e-mail your resume. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Email:tajib...@echd.org Tel:432-640-2348

Re: [Histonet] mast cells for arteries

Go to http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm for a reliable method. It still uses toluidine blue, but the staining is not based on metachromasia and mast cell granules are dark blue. Most toluidine blue samples will work, but occasionally one doesn't. Fisher brand is OK as

RE: [Histonet] Automated coverslipper with Ventana labels

I use to have a problem with the labels from the Ventana slides on the Leica Coverslipper. I now use the Trubond 200 slides which are a little wider (76x26mm instead of the standard 76x25mm) this keeps the labels from hanging off the sides and it seems to work on the Leica Coverslipper. Sandra

[Histonet] Heat Extractor for Frozen Sections

During residency, we prepared frozen sections by placing OCT and tissue on a precooled specimen disc and then putting a heat extractor on top, which both rapidly froze the tissue and created a flat surface. This seemed to work best when the heat extractor was slightly larger in diameter than

Re: [Histonet] mast cells for arteries

A good one that I used to use was the Naphthol AS-D Chloroacetate-esterase which stains the non specific esterase activity in mast cells. It is a pain to run, but it is one of the most beautiful. A bright red mast cell against a pale background. We got it from Vacca, p 530-533. On Tue, Nov 1,

Re: [Histonet] Problems with Frozen tissues

Igor, I perform Mohs which is a frozen section procedure. There are a couple of things that could help you. You do not have to leave the slides out to airdry for an HE only do this is a specfic procedure requires it. Do not places slides strait into water. As Kim, stated some people use a

[Histonet] AUTO: Gina Rodriguez is out of the office. (returning 11/07/2011)

I am out of the office until 11/07/2011. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or tech.supp...@leica-microsystems.com Note: This is an automated response to your message Histonet Digest, Vol 96, Issue 1 sent on 11/1/2011

RE: [Histonet] Problems with Frozen tissues

Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my HE with 1 min running water and so on. I rarely lose sections with these slides. Daniela

[Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA

Re: [Histonet] Problems with Frozen tissues

may I ask why you are not fixing them? Even the stain the 1st gentlman was doing(Beta Gal) recomended to have a formalin fixation apllied first.   There are many ways to get to the same show as we all know, But in my  experiance air drying frozen sections will dry out and cells can autolyse as

Re: [Histonet] Luciferase IHC

You have to soak it in holy water, then all H311 will break out   Sorry, I couldnt resist.     From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC

[Histonet] DAB Immuno and alcian blue counter staining

Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela __ This email has been