Hi everyone:
I want to detect immune cells (natural killers) in mouse's decidua, I
wonder if somebody knows a good antibody for that.
thanks!!!
Carmen
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Hello,
I would like to thank everyone who has replied to my post. Your
comments and advice are most helpful.
Kind regards
--
Tyrone Genade
http://tgenade.freeshell.org
email: tgen...@gmail.com
tel: +27-84-632-1925 (c)
Begin forwarded message:
From: Jesus Hernandez jesus.w@gmail.com
Date: November 4, 2011 4:41:20 PM CDT
To: histonet@lists.utsouthwestern.edu
Subject: Formic Acid Recipe
Dear all,
I was having trouble staining Human embryonic palatal mesenchymal cells with
multi-stain solution.
Can I do HistoQip if I am CLIA certified or does it have to be a CAP lab?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, November 06, 2011 2:17 PM
To: 'Janice Mahoney';
I have my paraffin chambers set at 60 degrees (embedding center and processor)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Friday, November 04, 2011 11:00 PM
To: Zoe rosa
Cc:
I believe Celestine Blue was used much more frequently in the UK in the 70s and
80s, preferred to an iron hematoxylin, in many trichrome stains including
Lendrum's MSB, as Peggy mentions; the nuclear staining withstanding further
differentiation during the subsequent staining from either picric
Thanks Peggy for the helpful information about recent commercial haemalum
substitutes.
The MSDS for tango describes only an acidified solution of the dye (eriochrome
cyanine R). This would need to be mixed with a ferric salt to make a blue
nuclear stain. Iron-ECR is probably the best
I was listening to the Wait Wait Don't Tell Me radio show this weekend and they
had to come up with the derivation of True Blue as a saying. Turns out it had
to do with blue dye colorfastness. Later they would call it Fast Blue - but
that does not convey the later meaning of the phrase..
Try Tech One Biomedical at www.techoneweb.com or 1-866-497-3033. They do good
work.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf
Interesting Tim
My true blue story dates from several years back when I was working up some
multiplex IHC staining using 2 HRP substrates.
One was DAB and the second was True Blue Substrate from KPL Labs. It worked
quite nicely, but is alcohol/xylene soluble so one needs only to wash in
That's pretty cool!!! I never knew...
Loralei
On Mon, Nov 7, 2011 at 10:09 AM, Morken, Timothy
timothy.mor...@ucsfmedctr.org wrote:
I was listening to the Wait Wait Don't Tell Me radio show this weekend and
they had to come up with the derivation of True Blue as a saying. Turns
out it had
Hello
I am currently studying to become a histotech. I wanted to know why should
the paraffin in the microwave processor be kept at 84 degrees.
Thanks
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From: chesk...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Subject:
Date: Mon, 7 Nov 2011 11:32:09 -0800
Hello
I am currently studying to become a histotech. I wanted to know why should
the paraffin in the microwave processor be kept at 84 degrees.
Thanks
Thank you for the great explanation along with a little history :-)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, November 07, 2011 12:09 PM
To:
I love Wait, Wait, Don't Tell Me! Interesting bit of trivia.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
Message: 12
Date: Mon, 7 Nov 2011 09:48:01 +0100 (CET)
From: Carmen Maria Garcia Pascualcarmen.m.gar...@uv.es
Subject: [Histonet] Immune cells antibody
To: histonet@lists.utsouthwestern.edu
Message-ID: 2427631794carma...@uv.es
Content-Type: text/plain; charset=ISO-8859-1
Hi everyone:
I
In Australia, True Blue has other meanings
From the website: http://www.malleeboy.com/music/true_blue_21.html
An Australian country western singer named John Williamson wrote a song
entitled True Blue
Definition of True Blue: Steadfast loyal Australian who displays the Aussie
ideals of a
One of our Lab Managers asked me to throw this topic to the Histonet.
Who is transporting surgical specimens between sites and are you using a
tracking system, if so what is it? Also, do you use a hired courier or
have a department courier system?
I will forward all replies to the manager.
There is a very
simple method to establish the real melting point of your paraffin.
You fill with melted paraffin a glass capillary and you allow
it to solidify.
Then you dip the
capillary into a porcelain capsule full of water in which the bulb of a
thermometer is plunged.
You slowly heat the
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