Dear all
I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.
Is it possible to reveal asbestos by means of electron microscopy?
Usually
The StainsFile page has some techniques, using polarizing microscopes.
http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm
Asbestos are usually very small fibers, and will not show up in every
section. Cutting thicker sections sometimes helps.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont
Martha,
They are now sold through HistoBiotec in the USA.
Jo Mauger
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha
Ward-Pathology
Sent: Monday, November 21, 2011 4:28 PM
To:
Hello...
I miss you so much This is for your eyes only
http://stereotyp.home.pl/profile/61DavidHarrison/
talk to you later
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Yolanda,
Yes it is, TEM is often used for asbestos detection in lung tissue to identify
chrysotile and/or crocidolite (mesothelioma-associated) asbestos fibers.
There are a lot of publications out there...
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck Co., Inc.
PO Box 4,
Many years ago asbestos fibers were demonstrated in hypochlorite digested
tissues. The remaining material was filtered through Milllipore filter, washed
and the fibers observed with polarized light.
The Perl's method will stain no the asbestos fibers (that cannot be stained)
but a reactive
To the person who asked about asbestos..
Not sure if digestion would have anything to do with what you are trying to do,
but
Dr. Victor Roggli at Duke performs this procedure (and interprets the results).
See
http://pathology.mc.duke.edu/website/WebForm.aspx?id=PulmonaryPathMain
He might
Lcda. Mary V. Guerrero,BS, MBA,HtL
Administradora/Coordinadora General
Pathology Lab.
55 N. Dr. Basora Edificio Médico IV Oficina 206
Mayaguez, Puerto Rico 00680
Tel. 787-834-8202 Fax: 787-831-5255
Sra. Dimary Valentín Sra. Iris Franqui Sra.
I just learned that the heating element for the paraffin bath on our old
Shandon embedding center has failed, and the company no longer supports the
older models. Does anyone have a source for that part they could recommend?
Many thanks
Hal Hawkins
UTMB Galveston and Shriners Hospital
Thanks to all who answered my question.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Montag, 21. November 2011 19:50
An: histonet@lists.utsouthwestern.edu
Betreff:
Rene,
I am interested to learn more of your experience with isopropanol as a
clearing agent.
Does it take longer to clear than xylene?
For processing how many stations and how long are the times you use for
clearing?
How often do you maintain or change isopropanol - how many blocks per liter?
I will be out of the office starting 11/22/2011 and will not return until
11/28/2011.
In my absence please ask for Mary . If this is urgent or you need to speak
to me directly you can contact me on my cell phone number 858-472-4266. If
it concerns a Mohs to be scheduled you can e-mail me or
We use the CBG recycling system - we have 1 for formalin and 1 for alcohol and
xylene. They are workhorses and we have had very little issue with them.
Our solvent recycler is run every day - we rotate a week at a time between
xylene and alcohol.
I have seen a couple of job postings that listed CLIA certification for
grossing as a requirement. Can someone give me some information on that
certification. I am currently doing gross in our derm office and I need to
know if I need to be certified.
Thanks
HR
This links to an article since the language of the regulation is dense, hits
the high points.
http://laboratory-manager.advanceweb.com/Features/Article-2/The-Grossing-Histotechnologist-in-Surgical-Pathology.aspx
Joelle Weaver MAOM, BA, (HTL) ASCP
http://www.linkedin.com/in/joelleweaver
Steve:
Of all the concerns you have, the fundamental should be that xylene is very,
very dangerous from the health point of view.
Under separate cover I am sending you 3 articles that answer your fundamental
questions.
Ah, by the way, you do not need xylene to dewax sections, just use a 2% aq.
Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues?
Interested in opinions/observations on staining patterns and sub-cellular
localization
Thanks in advance and happy thanksgiving to all
Luis Chiriboga Ph.D, Director
OCS Experimental Pathology IHC Core Lab
NYU School
Does this antibody detect little lies?
Sorry, short week and am anticipating the turkey!
Claire
Subject: [Histonet] Fibulin 3
Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues?
Interested in opinions/observations on staining patterns and sub-cellular
Dear Friends,
I am trying to section 25-50um thick paraffin sections of mouse brain. I
had success with 40um thick tumor sections but brain is very difficult to
section as such thick sections. I always use ParaPlast plus melting at 56 C.
Any suggestions on how to cut thick paraffin sections? Is
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