RE: [Histonet] GLUT-1 antibody

2011-11-29 Thread Hoekert, W.E.J.
Hi Sandra, I am using Neomarkers (Glut 1 Ab-1, polyclonal) on the Benchmark XT. 30' cc1 32' ab incubation 1:50 It works fine. Willem Hoekert OLVG Amsterdam The Netherlands Van: histonet-boun...@lists.utsouthwestern.edu namens Diaz, Sandra

[Histonet] Ahh...

2011-11-29 Thread Sarah Dysart
So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today. They are mouse samples, so smaller than others. Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and

[Histonet] RE: Ahh...

2011-11-29 Thread Weems, Joyce
That is what I would do. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: Ahh...

2011-11-29 Thread Beckham, Sharon
We do that a lot and have no problem at all. Sharon Beckham Head, Histotechnology Stowers Institute for Medical Research 1000 E 50th St Kansas City, MO 64110 816-926-4305 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Ahh...

2011-11-29 Thread Rene J Buesa
Yes, you can do that but the best way is NOT to take out the individual cassettes and let the solidify individually at RT. Prepare a container, fill it with melted paraffin and place all the cassettes inside. Then let that gigantic-common block to solidify. Melt it completely before doing the

[Histonet] nuclear fast red

2011-11-29 Thread Patsy Ruegg
Is my nuclear fast red powder dead or does it take a long time with heat and stirring to turn red? It is pretty old, and one vial says it should be stored below 0 but has not been. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite

RE: [Histonet] nuclear fast red

2011-11-29 Thread Elizabeth Chlipala
Patsy It does not go completely in solution we filter prior to staining Liz From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg [pru...@ihctech.net] Sent: Tuesday, November 29, 2011 11:29

[Histonet] Histologist needed in Austin Texas

2011-11-29 Thread Esparza, Sandra
Full Time/Day Shift position open for a HT/HTL (ASCP) registered histologist, with 2+ years working experience. Requires strong embedding and microtomy skills, knowledge of muscle enzymes, EM, and IHC is preferred. Proficiency in these areas can be obtained on the job. We are a highly

[Histonet] validation

2011-11-29 Thread Amos Brooks
Hi, I would say no, and if any inspector disagreed you would be well within your rights to give him a good cuff upside the head. The point in having a negative mouse and rabbit is to run them at the same concentration as whatever primary you are running. That would mean you would need to

[Histonet] autostainer vials

2011-11-29 Thread Thomas Pier
I'm using a Labvision Autostainer 360. I typically wash and reuse the reagent vials. Does anybody out there have any idea how long the vials will last doing this? Is there anything I'm not thinking of that I should be concerned about? Tom ___ Histonet

[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.

2011-11-29 Thread Diane . Tokugawa
I will be out of the office starting 11/29/2011 and will not return until 12/05/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408, Kiran at

[Histonet] Question regarding cutting on microtome

2011-11-29 Thread Marcia Spencer
I am wondering what causes a ribbon appearance in skin when viewed under the microscope, usually seen in the epidermis, but occasionally can be seen in the underlying tissue as well. I find this effect happens only in a few, 4 or 5 slides out of 100, but, I don't know how to correct it. I would