[Histonet] Fresh specimens after hours

We are not staffed 24/7. The OR here has a supply of small pre-filled formalin containers that they actually take into the OR room but not in the sterile area. They put the specimens directly in them and then, at the end of the procedure, deliver them just outside our Frozen Section room for

RE: [Histonet] Fresh specimens after hours

Just be sure there is a log book or computer entry system for the OR to log in the fact that they are leaving a specimen. Otherwise, specimens can disappear with the lab totally unaware. It happened to us in my previous position. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225

[Histonet] SOP for validation

Would anyone be willing to share their SOP for validation? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cost of 10% NBF

I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here

Re: [Histonet] Cost of 10% NBF

Regardless of any economic consideration, NBF should NEVER be prepared in the lab. The exposure of the personnel is too high and dangerous and ought to prevail over costs savings. Many, many years ago we also prepared our own NBF but abandoned that practice also many many years ago. Buy the NBF

[Histonet] RE: Cost of 10% NBF

Sorry Sara, We make our own here too. I can't remember why exactly. One reason is the space for a 50 gallon drum I believe. Good Luck. Bureaucrats always think personnel is cheap enough to be expendable. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451

[Histonet] b-cells stain

Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions

RE: [Histonet] FW: EM questions

I would suggest using some sort of conductive adhesive for what I guess is analysis of the minerals by scanning electron microscopy and energy-dispersive X-ray analysis of their elemental composition. I would think that embedding in a high melting point wax might have adverse effects by

[Histonet] Upper Level Histology Positions

I am a nationwide laboratory recruiter and I have several histology supervisor and manager level positions located nationwide. All positions offer great salaries and relocation. For more info contact me, Kaitlin at 301.693.9057. Kaitlin Webster Account Manager Prometheus Healthcare Office

[Histonet] Rabbit antibody on rabbit tissue

Hi All, I am going to apply one rabbit polyclonal antibody on the rabbit tissue using IF. Is there anybody do it before? I heard of mouse on mouse IHC-DAB method, but not sure if it's possible in IF. Your kind response is very appreciated, Thanks, Karen

[Histonet] identification of Protheca

Dear Histonetters: One of my pathologists is seeking a method to identify and speciate Prototheca in fixed animal tissues. I can't seem to find anyone that does this. Any ideas? Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory ___

Re: [Histonet] b-cells stain

Greetings Silvina: You do not have to use paraldehyde, you can make a perfectly good aldehyde fuchsin using acetaldehyde since one molecule of paraldehyde depolymerizes into 3 molecules of acetaldehyde. Just use 3x the amount of acetaldehyde. n-butyraldehyde will also work for b-cells. See

RE: [Histonet] Cost of 10% NBF

I agree with Rene'. Many years ago at a previous facility, I was wearing all of my PPE's, including a full-face respirator, gloves, and jumpsuit while making 10% nbf. However, the 37% formalin (which was being pumped in with a hose) splashed on my protective jumpsuit. The incident caused

[Histonet] Formalin Pricing Follow-up

Thanks to everyone who gave me Peer Review on my make-formalin-vs-buy-formalin. I appreciate everyone's input and with all the comments, I think I've convinced Our Fearless Leader to go with the 55-gallon drum idea. Thank you, everyone! Sally Breeden, HT(ASCP) New Mexico Department of

[Histonet] b-cells stain

Hi, I like to prepare solutions myself as well. As you say though paraldehyde is classified as a drug so there are hoops to jump through to be able to purchase it. This is one of those times that it totally makes sense to let someone else make it up. EMS sells aldehyde fuchsin so it saves me

[Histonet] pan-cathepsin antibody

Hi all- I'm looking for a pan-cathepsin antibody for FFPE IHC and can only find Santa Cruz sc-6499. Has anyone used this, or other pan cathepsin antibodies? Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486

Re: [Histonet] b-cells stain

Acetaldehyde can be used instead of paraldehyde, to make aldehyde fuchsin. Substitute 1.5 - 2.0 mL acetaldehyde for every 1.0 mL of paraldehyde. Acetaldehyde is usually not a restricted drug, a whole lot cheaper than acetaldehyde, and once opened, remains good for much longer than paraldehyde,

Re: [Histonet] identification of Protheca

One minute with Google turned up this reference. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC380951/pdf/applmicro00058-0151.pdf It will have been cited by many other papers. Your pathologist boss should be able to find them on a cited reference search web site. The best ones are Web of